Protein Alignment NKCC and slc12a4

DIOPT Version :10

• Sequence 1: NP_730938.1
• Gene: NKCC / 40663
• FlyBaseID: FBgn0051547
• Length: 1068
• Species: Drosophila melanogaster
• Sequence 2: XP_002942716.1
• Gene: slc12a4 / 100493471
• XenbaseID: XB-GENE-1014107
• Length: 1090
• Species: Xenopus tropicalis
• Alignment Length: 1246
• Identity: 308/1246 - (24%)
• Similarity: 487/1246 - (39%)
• Gaps: 341/1246 - (27%)

  Fly     5 FQISKVNGTANAGYDGEARSAESDQTDAAH----PLPPTSNENHLHPKASGETGENRRSSRLSFR 65
            |.:..|...|..|.|........|..|..|    |....|::.|      |...||......|..
 Frog     4 FTVVPVEEQARLGCDYLEGLNGVDYRDTGHSSRDPYDSVSSDGH------GNYKENSPFLTGSEA 62

  Fly    66 GFGN-FLRKSDA--ERKFSLAQLTKESLPRLDNYRISMRNLKRPSIGELQGEAVDQSITIPEPEP 127
            ..|| |..|:.|  |.:..:.......|.||.||....:..|.....|.....|::|..
 Frog    63 NKGNQFYDKNLALFEEELDIRPKVSSFLSRLVNYTNLTQGAKEHEEAEEARRKVNKSPC------ 121

  Fly   128 EATGGHIKLGWIVGVLIPCLLNIWGVMLFLRLSWVVAESGILQSLIIITISAVVCVITTLSLSAI 192
                    :|.::||.:||:.||.||:|||||:|:|...|:||:|:|..|.....::|.:|:|||
 Frog   122 --------MGTVMGVYLPCMQNILGVILFLRLTWIVGTGGVLQTLLITLICCCCTLLTAISMSAI 178

  Fly   193 STNGEVKGGGVYFIISRSLGPEFGASVGVVFAFANAVSASMNTIGFCESLNVLL----------- 246
            :|||.|..||.||:||||||||||.:||:.|......:.:|..:|..|.|.:.:
 Frog   179 ATNGVVPAGGSYFMISRSLGPEFGGAVGLCFYLGTTFAGAMYILGAIEILLMYIVPEAAIFHSPN 243

  Fly   247 --KNNDLKIVDNGINDIRIVGSITVLVLILICCVGMEWETKAQNFLIVTIVLAIFNFLIGA---- 305
              :::..|     :|::|:.|::.:.::.:|..||:::..|..:..:..::::|.:...|:
 Frog   244 THEDSSAK-----LNNMRVYGTVFLTLMAIIVFVGVKYVNKFASLFLACVIISILSIYAGSIKSI 303

  Fly   306 ------AIGPQGNEEQISR---------------------------------------------- 318
                  .|...|| ..:||
 Frog   304 FDPPVFPICMLGN-RTLSRDRFEICAKTIILDNVTKPTTLWEQFCGSSNFSTSSCDQYFLKNNVT 367

  Fly   319 ---GFVGFSWATLKENFGSDYRYAEG---------------------------VNHDFFSVFAIF 353
               |..|.:...||:|..|:| ..:|                           ::..|..:..||
 Frog   368 EIAGIPGAASGILKDNLWSNY-LQKGEILEKSQLSSVDELGSKSNLHLYVLTDISATFTVLVGIF 431

  Fly   354 FPSVTGIQAGANICGDLKDAGAAIPKGTFWSLL----ISMSSYALFVLFAGGAAVRDASGIPADL 414
            |||||||.||:|..|||:||..:||.||..:::    |..|:..||.....|..:||..|   |.
 Frog   432 FPSVTGIMAGSNRSGDLRDAQKSIPVGTILAIITTSFIYFSTVVLFGACIEGVVLRDKYG---DA 493

  Fly   415 VNGTIVSSELPCMATGNCTWGLFNSYEMMQEMSLWGPLIYAGCFAATLSTALTNLLSVPRLVQAL 479
            ||..:|        .|..:|           .|.|  :|..|.|.:|....|.:|...|||:||:
 Frog   494 VNKNLV--------VGTLSW-----------PSPW--VIVIGSFFSTCGAGLQSLTGAPRLLQAI 537

  Fly   480 GIDQIYPGLIFFSKPYGK-HGEPYRGYVLTFFITTGFLLIGELNLIAPLISTFYLASYALINFCT 543
            ..|.|.|.|..|.:  || :|||....:||.||....:||..|:::||::|.|:|..|..:|...
 Frog   538 ANDNIIPFLRVFGQ--GKVNGEPTWALLLTAFIAELGILIASLDMVAPILSMFFLMCYLFVNLAC 600

  Fly   544 FHAAFVKPLGWRPTFKYYNAWLSLFGFAMCVAIMFLINYVAAIITFGIIFALYLVVMYRKPEANW 608
            .....::...|||.||||:..||.||.::|:|:||:.::..|:|...|...:|..:.|:..|..|
 Frog   601 AVQTLLRTPNWRPRFKYYHWALSFFGMSICLALMFVCSWYYALIAMLIAGMIYKYIEYQGAEKEW 665

  Fly   609 GSTTQAQQYKAALMAVHRLQNVSDHVKNYHPQVLV---LSGDPKTR-PPLVDFGYLLTKNNSLMF 669
            |...:.....||..|:.||:....|.||:.||:||   |..|...: |.|:.|...|.....|..
 Frog   666 GDGIRGLSLSAARYALLRLEEGPPHTKNWRPQLLVLLKLDEDLHVKYPRLLTFSSQLKAGKGLTI 730

  Fly   670 VANIIPVRV--GYKNRQHLVKDGQKYLDARKIKAFYNVIDGFSLEDGINALTKSTGFGKMSPNIV 732
            :...:....  .|...|...:..:..::..|:|.|..|:....:.|||:.|.:|.|.|.|..|.|
 Frog   731 IGTTLQGNFLESYGEVQAAEQTIKNMMEIEKVKGFSQVVVSSEVCDGISHLIQSCGLGGMKHNTV 795

  Fly   733 LVGYKPDWNRCRKEEVESYFSILYNAFSQRMGVALLRLPNGLDFSELSSEVTLPANGMGHMHTAN 797
            |:|:...|.  :.|:..|:.:.:
 Frog   796 LLGWPYGWR--QSEDPRSWKTFI------------------------------------------ 816

  Fly   798 AHGFTNELMPAANAASELLHIDSNLNLASMDSPNSSFTMPQPAPMPNMQRNSRSYKVTSSDEPAV 862
                  :.:....||          .||.|...|.||
 Frog   817 ------DAVRCTTAA----------RLALMVPKNVSF---------------------------- 837

  Fly   863 TYHTKGGSDIPQNLLDAMTIFTRKQPKGTIDVFWLYDDGGLTILLPYIISMRSHWQNSKLRVFAM 927
                     .|.|        ..:..:|.|||:|:..|||:.:|||:::.....|:..|:|:|.:
 Frog   838 ---------YPSN--------HERYLEGNIDVWWIVHDGGMLMLLPFLLKQHKVWKKCKMRIFTV 885

  Fly   928 CHGKDEE-QEEKSMASLLTKFRIK-----------------YSELIMLKGVS-----------EQ 963
            ....|.. |.:|.:|:.|...|::                 |...:|::..|           |:
 Frog   886 AQLDDNSIQMKKDLATFLYHLRLEAEVEVVEMHNSDISAYTYERTLMMEQRSQMLRQMRLTKTER 950

  Fly   964 PRADTVLKHKRLI-------------EPFRRGARNEFGITDDELQSMSEKTNRQ----------- 1004
            .|...::|.:..|             ||....|:.....|.|:..:..:..|.:
 Frog   951 DREAQLVKDRNSIIPVESYYSDEEDEEPELELAKIHMTWTKDKYNAQWKNRNNETMHFRELADIK 1015

  Fly  1005 ------------LRIHELVVKHSSNASLVVMSLPMPRKEAISAPLYMSWLEMLTSDMKCPVALAR 1057
                        ::::|::|..|.:|.||::::|.|.|.......||.:||:||..:: .|.|.|
 Frog  1016 PDQSNVRRMHTAVKLNEVIVTRSHDAKLVLLNMPGPPKNTDGDENYMEFLEVLTEGLE-RVLLVR 1079

  Fly  1058 GNQTPVLTLYS 1068
            |....|:|:||
 Frog  1080 GGGREVITIYS 1090

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
NKCC	NP_730938.1	AA_permease_2	85..1068	CDD:459263	285/1157 (25%)
slc12a4	XP_002942716.1	2a30	49..1090	CDD:273347	294/1193 (25%)