Protein Alignment NKCC and slc12a1

DIOPT Version :10

• Sequence 1: NP_730938.1
• Gene: NKCC / 40663
• FlyBaseID: FBgn0051547
• Length: 1068
• Species: Drosophila melanogaster
• Sequence 2: XP_002936613.2
• Gene: slc12a1 / 100490198
• XenbaseID: XB-GENE-479925
• Length: 1086
• Species: Xenopus tropicalis
• Alignment Length: 1146
• Identity: 457/1146 - (39%)
• Similarity: 656/1146 - (57%)
• Gaps: 158/1146 - (13%)

  Fly     4 RFQISKVNGTANAGYDGEARSAESDQTDAAHPLPPTSNENHLHPKASGETGENRRSSRLSFRG-F 67
            |||::.|    ..|||..|    .:..|     ||...|...       ..:||.|.|...|. |
 Frog    18 RFQVNVV----GEGYDNPA----CEDKD-----PPDYEETSF-------ADDNRMSYRPGNREIF 62

  Fly    68 GNFLRKSD---AERKF-------------SLAQLTKESLPRLDNYR----ISMRNLKRPSIGELQ 112
            .|.|...|   .|..|             :....|.:::|::|.||    ||...:.|||:.::.
 Frog    63 NNVLHNGDTIKTEGSFHPYDTHGHAYYMKTFGHNTVDAVPKIDYYRNTGSISGVKICRPSLADIH 127

  Fly   113 GEAVDQSITIP---------------EPEPEATGGHIKLGWIVGVLIPCLLNIWGVMLFLRLSWV 162
            .:.....:.:|               ..|....||.:|.|||.|||:.|:|||||||||:||||:
 Frog   128 EQLSKNVVEVPGSVTNGEGIPGDEEANKEEGEKGGFVKFGWIKGVLVRCMLNIWGVMLFIRLSWI 192

  Fly   163 VAESGILQSLIIITISAVVCVITTLSLSAISTNGEVKGGGVYFIISRSLGPEFGASVGVVFAFAN 227
            |.::||...::||.:|.||..:|.:|:|||.|||.|:|||.|::||||||||||.|:|::|||||
 Frog   193 VGQAGIGLGVVIICLSVVVTSLTGVSMSAICTNGVVRGGGAYYLISRSLGPEFGGSIGLIFAFAN 257

  Fly   228 AVSASMNTIGFCESLNVLLKNNDLKIVDNGINDIRIVGSITVLVLILICCVGMEWETKAQNFLIV 292
            ||:.:|..:||.|::..:||:.:..:||. :|||||:|:|||::|:.|...|||||.|||..|:|
 Frog   258 AVAVAMYVVGFSETVVEILKDTNTIMVDE-VNDIRIIGTITVILLLGISVAGMEWEAKAQVVLLV 321

  Fly   293 TIVLAIFNFLIGAAIGPQGNEEQISRGFVGFSWATLKENFGSDYRYAEGVNHDFFSVFAIFFPSV 357
            .:::||.||.||..| |...|:: ::||..:......||||.|:|..||    ||||||||||:.
 Frog   322 ILLIAIVNFFIGTVI-PTTKEKR-AQGFFNYQATIFAENFGPDFRNGEG----FFSVFAIFFPAA 380

  Fly   358 TGIQAGANICGDLKDAGAAIPKGTFWSLLISMSSYALFVLFAGGAAVRDASGIPADLVNGTIVSS 422
            |||.|||||.|||||...||||||.|::||:..:|....:.|..:.||||:|    .:|.||.|:
 Frog   381 TGILAGANISGDLKDPQGAIPKGTMWAILITTIAYLGVAICAAASVVRDATG----NLNDTITSA 441

  Fly   423 -------------ELPCMATGNCTWGLFNSYEMMQEMSLWGPLIYAGCFAATLSTALTNLLSVPR 474
                         :.....|..|.:||.|::::|..:|.:||||.||.|:||||:||.:|:|.|:
 Frog   442 FKCNGSAACGLGYDFSICETQTCNYGLMNNFQVMSMVSGFGPLIIAGIFSATLSSALASLVSAPK 506

  Fly   475 LVQALGIDQIYPGLIFFSKPYGKHGEPYRGYVLTFFITTGFLLIGELNLIAPLISTFYLASYALI 539
            :.|||..|.||.||.||:|.:||:.||.|||||||.|...|:||.|||:|||:||.|:|||||||
 Frog   507 VFQALCKDNIYKGLHFFAKGHGKNNEPIRGYVLTFVIALAFILIAELNVIAPIISNFFLASYALI 571

  Fly   540 NFCTFHAAFVKPLGWRPTFKYYNAWLSLFGFAMCVAIMFLINYVAAIITFGIIFALYLVVMYRKP 604
            ||..|||::.|..||||.|||||.|:||||..:|..:||:||:.||:||:.|...||:.|.|:||
 Frog   572 NFSCFHASYAKSPGWRPAFKYYNMWVSLFGAMLCCGVMFVINWWAALITYAIELFLYIYVTYKKP 636

  Fly   605 EANWGSTTQAQQYKAALMAVHRLQNVSDHVKNYHPQVLVLSGDPKTRPPLVDFGYLLTKNNSLMF 669
            :.||||:|||.|:|.||.:...|..:.|||||:.||.:||:|.|.|||.|:|..:..|||:.|..
 Frog   637 DVNWGSSTQALQFKNALDSALGLNCIEDHVKNFRPQCIVLTGVPMTRPALLDITHSFTKNSGLCI 701

  Fly   670 VANII--PVRVGYKNRQHLVKDGQKYLDARKIKAFYNVIDGFSLEDGINALTKSTGFGKMSPNIV 732
            ...:.  |.:...|.....:...|.:|...|.||||..:...:..||:..|.:::|.|::.||.:
 Frog   702 CCEVFSGPRKQAVKEMNSGMIKKQTWLTNNKRKAFYAAVAADNFRDGVRTLLQASGLGRLKPNTL 766

  Fly   733 LVGYKPDWNRCRKEEVESYFSILYNAFSQRMGVALLRLPNGLDFSE---------------LSSE 782
            ::|:|.||.:....::.:|..||::||....||.::|:..|.|.|:               |:.|
 Frog   767 VMGFKKDWRQASPVDLANYVGILHDAFDFEHGVVIVRISQGFDISQVLQVKEEFDRLEQERLALE 831

  Fly   783 VTLPANGMGHMHTANAHGFTNELMPAANAASELLHIDSNLNLASMDSPNSSFTMPQPAPMPNMQR 847
            .|:..|   .....| ||    |....|..::|     |:|..|     |:..:..|.||...:.
 Frog   832 QTIKEN---EFKEGN-HG----LRSFFNKTAKL-----NINRHS-----STKDVSTPPPMHVGEY 878

  Fly   848 NSRSYKVTSSDEPAVTYHTKGGSDIPQNLLDAMTIFTRKQPKGTIDVFWLYDDGGLTILLPYIIS 912
            |                         |.|::|...|.:.|.||.|||:||:||||||:|:|:|::
 Frog   879 N-------------------------QRLVEASEQFKKNQGKGMIDVWWLFDDGGLTLLIPHILT 918

  Fly   913 MRSHWQNSKLRVFAMCHGKDEEQEEKSMASLLTKFRIKYSELIMLKGVSEQPRADTVLKHKRLIE 977
            :|..|::.|||:|........|.|:..|||||:|||||::::.::..::.:|..::....:.:||
 Frog   919 LRKKWKDCKLRIFIGGKVNRLEDEKLMMASLLSKFRIKFADIYIVGDINTKPSKESWKFFEEMIE 983

  Fly   978 PF---------------RRGARNEFGITDDELQSMSEKTNRQLRIHELVVKHSSNASLVVMSLPM 1027
            |:               ||  .|.:.|||.||:...||:.||:|::||:.:||.:|:|:|:|||:
 Frog   984 PYCLHENSTDVSTAEKIRR--ENPWKITDSELEMFKEKSYRQVRLNELLQEHSRSANLIVLSLPV 1046

  Fly  1028 PRKEAISAPLYMSWLEMLTSDMKCPVALARGNQTPVLTLYS 1068
            .||.::|..|||:|:|:|:.::. ||.|.|||...|||.||
 Frog  1047 ARKGSVSDHLYMAWVEILSKNLP-PVLLVRGNHKNVLTFYS 1086

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
NKCC	NP_730938.1	AA_permease_2	85..1068	CDD:459263	432/1046 (41%)
slc12a1	XP_002936613.2	2a30	96..1086	CDD:273347	432/1046 (41%)