Protein Alignment ksr and araf

DIOPT Version :10

• Sequence 1: NP_524236.2
• Gene: ksr / 40660
• FlyBaseID: FBgn0015402
• Length: 966
• Species: Drosophila melanogaster
• Sequence 2: XP_031746913.1
• Gene: araf / 100495479
• XenbaseID: XB-GENE-5755831
• Length: 644
• Species: Xenopus tropicalis
• Alignment Length: 797
• Identity: 223/797 - (27%)
• Similarity: 324/797 - (40%)
• Gaps: 223/797 - (27%)

  Fly   220 RQHGVKGKNSALANSTNFKSGRQSPSATEELNSTQGSQLTLTLT----PSP----PNSPFTPSSG 276
            |.|...|   ||||..       ..|.:.||.  :||.:.|:..    |:|    |....:||.|
 Frog     3 RSHTPPG---ALANGA-------LHSLSRELG--KGSAVMLSAAVLELPNPGTRVPAQQESPSRG 55

  Fly   277 LSSS--------LNGT-----PQRSR---GTPPPARKHQTL--------LSQSHVQVDGEQLARN 317
            .|.|        ..||     |.:.|   ...|....:.:|        |||....|......|.
 Frog    56 ASVSEYPHAEGKSGGTVKVYLPNQQRTVVNVRPGMTVYDSLDKALKVRGLSQECCAVYRLIQGRK 120

  Fly   318 RLPTDPSPDSHSSTSSDIFVDPNTNASSGGSSSNVLM-VPCSPGVGHVGMGHAIKHRFTKALGF- 380
            .| ||...|.......::.|:             ||. ||.|            .|.|.:...|
 Frog   121 AL-TDWETDITPLVGEELMVE-------------VLADVPLS------------MHNFVRKTHFT 159

  Fly   381 MATCTLCQKQVFHRWMKCTDCKYICHKSCAPHVPPSCGLPREYVDEFRHIKEQGGYASLPHVHGA 445
            :|.|..|.|.:||.: :|..|.|..|:.|:..||.:|      ||          .|.||
 Frog   160 LAFCDFCLKFLFHGF-RCQTCGYKFHQHCSSKVPTAC------VD----------MAKLP----- 202

  Fly   446 AKGSPLVKKSTLGKPLHQQHGDSSSPSSSCTSSTPSSPALFQQRERELDQAGSSSSANLLPTPSL 510
               .||.       ||..|  |..|.....|...|.||   |.........||:|.:  .|
 Frog   203 ---KPLT-------PLSYQ--DFQSYYEHPTVHLPLSP---QPPSPRTQHPGSASFS--FP---- 246

  Fly   511 GKHQPSQ----FNFPNVTVTSSGGSGGVSLISNEPVPEQFPTAPATANGGLDSLVSSSNGHMSSL 571
            |:.||.|    .:.|||.:.|:.|.     :.|..:.|...:|..|...|
 Frog   247 GEGQPLQRHRSTSTPNVHIDSTTGP-----VDNSIIEEALRSAAVTPVDG--------------- 291

  Fly   572 IGSQTSNASTAATLTGSLVNSTTTTSTCSFFPRKLSTAGVDKRTPFT---SEYTDTHKSNDSDKT 633
                                           |.| |...:..::|.|   ||.|...|::.||:.
 Frog   292 -------------------------------PPK-SAQPLGSKSPQTKSPSETTRERKTSASDEK 324

  Fly   634 VSLSGSASTDSDRTPVRVDSTEDGDSGQWRQNSISLKEWDIPYGDLLLLERIGQGRFGTVHRALW 698
            ..:......||              |..|..||          .::::|:|||.|.||||:|..|
 Frog   325 KKVKQLGYRDS--------------SYYWEVNS----------SEVIILKRIGTGSFGTVYRGKW 365

  Fly   699 HGDVAVKLLNEDYLQDEHMLETFRSEVANFKNTRHENLVLFMGACMNPPYLAIVTSLCKGNTLYT 763
            |||||||:|.......| .::.|::|:...:.|||.|::|||| .|..|..||:|..|:|::||.
 Frog   366 HGDVAVKILKVTNPTSE-QIQAFKNEMQVLRKTRHVNILLFMG-FMTRPQFAIITQWCEGSSLYR 428

  Fly   764 YIHQRREKFAMNRTLLIAQQIAQGMGYLHAREIIHKDLRTKNIFIENGKVI-ITDFGLFSSTKLL 827
            ::|....:|.:.:.:.||:|.||||.||||:.|||:||::.|||:..|..: |.||||.:.....
 Frog   429 HLHVIETRFDIFQLIDIARQTAQGMDYLHAKNIIHRDLKSNNIFLHEGLTVKIGDFGLATVKTRW 493

  Fly   828 YCDMGLGVPHNWLCYLAPELIRALQPEKPRGECLEFTPYSDVYSFGTVWYELICGEFTF-----K 887
            .....:..|...:.::|||:|| :|...|      ::..||||::|.|.|||:.|...:     :
 Frog   494 SGSQQVEQPSGSILWMAPEVIR-MQESSP------YSSQSDVYAYGVVLYELMAGLLPYANINNR 551

  Fly   888 DQPAESIIWQVGRG-MKQSLANLQSG--RDVKDLLMLCWTYEKEHRPQFARLLSLLEHLPK--KR 947
            ||    ||:.|||| :...|:.:.:.  :.:|.||:.|..:::|.||.|.::||.:|.|.:  .:
 Frog   552 DQ----IIFMVGRGYLTPDLSKVSNNCPKAMKRLLVNCLKFKREERPLFPQILSSIELLQRALPK 612

  Fly   948 LARSPSHPVNLSRSAES 964
            :.||.|.| :|.|:..|
 Frog   613 IERSASEP-SLHRAVNS 628

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
ksr	NP_524236.2	SAM_KSR1_N	33..86	CDD:466555
		SAM_KSR1	92..166	CDD:463916
		C1_KSR	370..418	CDD:410362	16/48 (33%)
		PK_KSR	678..946	CDD:270965	105/278 (38%)
araf	XP_031746913.1	RBD_ARAF	70..142	CDD:340653	16/85 (19%)
		C1_A_C-Raf	147..198	CDD:410420	20/79 (25%)
		Protein Kinases, catalytic domain	345..609	CDD:473864	105/276 (38%)