Protein Alignment PEK and Eif2ak4

DIOPT Version :10

• Sequence 1: NP_649538.1
• Gene: PEK / 40653
• FlyBaseID: FBgn0037327
• Length: 1162
• Species: Drosophila melanogaster
• Sequence 2: NP_038747.2
• Gene: Eif2ak4 / 27103
• MGIID: 1353427
• Length: 1648
• Species: Mus musculus
• Alignment Length: 1140
• Identity: 276/1140 - (24%)
• Similarity: 407/1140 - (35%)
• Gaps: 391/1140 - (34%)

  Fly   113 RLELTNNGQFVRMIPSLSGGIYKFDGDSIDPIPITAEHLLSSSAKFSDDLVISGGKETRSYGVSV 177
            |||:|          ||:...|   ....||....|..:|...   |.|.|.:|...|.|.|.|.
Mouse   200 RLEIT----------SLTNQDY---ASKRDPAGHRAAAILHGG---SPDFVGNGKARTYSSGRSR 248

  Fly   178 RTGQLLYE-CSLNGCVNSTEEGLAIDDTIREPDEEDQLEDGEQLRDEAGYIVRHDPLLDDVIIVR 241
            |..|  |. ||......|.:   .:..::..||         ||....|..|..|..|..|:
Mouse   249 RERQ--YSVCSGEPSPGSCD---ILHFSVGSPD---------QLMVHKGRCVGSDEQLGKVV--- 296

  Fly   242 RQTQTVRAVESRTGVERWNFSVGQHE--LDLVRPSEC-QLQPRDELELAVLDVDIKVVVPEGIIC 303
                 ..|:|:.||    :| |..||  |...:...| ..|.:::::.....:       :|...
Mouse   297 -----YNALETATG----SF-VLLHEWVLQWQKMGPCLTSQEKEKIDKCKRQI-------QGAET 344

  Fly   304 AFSKSEPQTMLWKYKFDHPIV---SAWNTNADDELQPIDLFSSAQWLWDQDENDTELPNAP---- 361
            .||.        ..|..||.:   .|.|:..:::...||:.:         |:.:.:..|.
Mouse   345 EFSS--------LVKLSHPNIVRYFAMNSREEEDSIVIDILA---------EHVSGISLATHLSH 392

  Fly   362 QSP-PSIYLGMYDKQLYIQESIRLRQEIMDQTKVYQQLTG-DTSLMPRIPWKPISASSKSLVIFR 424
            ..| |:..|..|..||                     |.| |......:..|.:||||   |:
Mouse   393 SGPVPAHQLRKYTAQL---------------------LAGLDYLHSNSVVHKVLSASS---VL-- 431

  Fly   425 KDQEDPEMIAEGAVAQGGELVPYD-DENFA-VAAQSVLNASEFVNGNGFYFYTTG---------- 477
                   :.|||.|    ::..|. .:..| :..:.|...:.....:....|.||
Mouse   432 -------VDAEGTV----KITDYSISKRLADICKEDVFEQARVRFSDSALPYKTGKKGDVWRLGL 485

  Fly   478 ---DLNGPQECSTQ--NNPTDLPAITAPTSPTNATSEGTEATGNHSVNDDLGFSLDDIDAPVKVV 537
               .|:..|||...  ..|:||||                               |..|...|.|
Mouse   486 LLLSLSQGQECGEYPVTIPSDLPA-------------------------------DFQDFLKKCV 519

  Fly   538 ILSLWFWWKEIVVIAFTSAVILNIFMGQRNQRVEREYLVIERHVP-VQTAIEATEASTQALLGPV 601
            .|.....|..                   .|.::..::..:..:| |:.:.|  ::..|..:..|
Mouse   520 CLDDKERWSP-------------------QQLLKHSFINPQPKLPLVEQSPE--DSGGQDYIETV 563

  Fly   602 VPM-QRPGNRFSFPPGQANQRTISESTTHSGEHYTSRFQSDFELMQCLGRGGFGVVFEAKNKLDE 665
            :|. |.|...|           .||:     :...||:..:||.:|.||:|.||.|.:.:||||.
Mouse   564 IPSNQLPSAAF-----------FSET-----QKQFSRYFIEFEELQLLGKGAFGAVIKVQNKLDG 612

  Fly   666 NRYAIKRITLPNKESSR--QRVLREARTLASCEHHNIVRYFHSWTE----------TPPTGWQEE 718
            ..||:|||  |...:||  :|:..|...|:...|.|||||:::|.|          .||....:.
Mouse   613 CCYAVKRI--PINPASRHFRRIKGEVTLLSRLHHENIVRYYNAWIERHERPAVPGTPPPDCTPQA 675

  Fly   719 EDRKLLAHELS------TSIQIETPDDSTMPSLTEQLKEKRQQQLLSWVSDAANSTACSHDFHLP 777
            :|......:.|      .|::...|.    |.|:..::         |.:.|..||  |..|.:.
Mouse   676 QDSPATCGKTSGDTEELGSVEAAAPP----PILSSSVE---------WSTSAERST--STRFPVT 725

  Fly   778 GESSLKNIREEYDYDEEEDSLIEFRSESQSAALRAEEEDDTDDDYEEDEEQQGDHEKRHRSSVSI 842
            |:                                     |:..|.|:::|:.|
Mouse   726 GQ-------------------------------------DSSSDEEDEDERDG------------ 741

  Fly   843 DIHSASFDLKNINYSQHQLVSNSFQIESVRPKSSGSDDA---NDDNKARRKPLTLALAQNHN--- 901
             :.|.||                      .|.|....|.   |:|..::        :||.:
Mouse   742 -VFSQSF----------------------LPASDSDSDIIFDNEDENSK--------SQNQDEDC 775

  Fly   902 NNQNGSQPTPSSATILNGTVAKPSKVYLYIQMQLCRKESLRDWL-----RDNRSETRAAHIGDIF 961
            |.::||.....|.|       ..:..||||||:.|.|.:|||.:     ||      .:.:..:|
Mouse   776 NQKDGSHEIEPSVT-------AEAVHYLYIQMEYCEKSTLRDTIDQGLFRD------TSRLWRLF 827

  Fly   962 HQIVDAVDYVHLKGLIHRDLKPSNIFFSQDGQIKIGDFGLVTDMADIPNLVAKCGDQSG------ 1020
            .:|:|.:.|:|.||:|||||||.|||...|..:|||||||.||.... ....|..||:|
Mouse   828 REILDGLAYIHEKGMIHRDLKPVNIFLDSDDHVKIGDFGLATDHLAF-TAEGKQDDQAGDGVIKS 891

  Fly  1021 LPSCARHTQQVGTHLYMSPEQLLG---QHYDYKVDIYSLGLIFFELHVY-FSTEMERIKTLRSLR 1081
            .|| ...|..|||.||:||| :.|   ..|:.|||::|||:||||:..: ..|..|||..|..||
Mouse   892 DPS-GHLTGMVGTALYVSPE-VQGSTKSAYNQKVDLFSLGIIFFEMSYHPMVTASERIFVLNQLR 954

  Fly  1082 DGQYPKDFAVNYPQQYD---------LLQQMLSAQPEQRPQTKQ-LKSQLRNILQLPHLLSEGQS 1136
            |...||     :|..:|         ::..:|:..|.:||...: |||:         ||...|.
Mouse   955 DPTSPK-----FPDDFDDGEHTKQKSVISWLLNHDPAKRPTAMELLKSE---------LLPPPQM 1005

  Fly  1137 EQAELAE-----------RARRLSRSRTFSSSSEP 1160
            |::||.|           :|.|...|:.|.....|
Mouse  1006 EESELHEVLHHTLANIDGKAYRTMMSQIFCQHISP 1040

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
PEK	NP_649538.1	Luminal_EIF2AK3	73..401	CDD:188874	65/300 (22%)
		Protein Kinases, catalytic domain	635..1113	CDD:473864	151/525 (29%)
Eif2ak4	NP_038747.2	Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1..26
		RWD_GCN2	20..138	CDD:467659
		PK_eIF2AK_GCN2_rpt1	299..537	CDD:270914	64/353 (18%)
		STKc_EIF2AK4_GCN2_rpt2	582..998	CDD:270948	154/533 (29%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	661..784		33/217 (15%)
		Histidyl-tRNA synthetase-like	1021..1492		5/20 (25%)
		PLN02972	<1070..1490	CDD:215525
		HGTP_anticodon	1396..1640	CDD:469699