DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment PEK and Eif2ak3

DIOPT Version :9

Sequence 1:	NP_649538.1	Gene:	PEK / 40653	FlyBaseID:	FBgn0037327	Length:	1162	Species:	Drosophila melanogaster
Sequence 2:	NP_034251.2	Gene:	Eif2ak3 / 13666	MGIID:	1341830	Length:	1114	Species:	Mus musculus

Alignment Length:	1275	Identity:	405/1275 - (31%)
Similarity:	594/1275 - (46%)	Gaps:	311/1275 - (24%)

- Green bases have known domain annotations that are detailed below.

  Fly    14 RSLSFLQIVTL-TMAGLVAFDPAQVL-------------AGHPTT---------------DSEL- 48
            |:|..|..:.| ..||:.|..||:.|             |..||:               |:|.
Mouse    10 RALLLLLFLLLGCAAGISAVAPARSLLAPASETVFGLGAAAAPTSAARVPAVATAEVTVEDAEAL 74

  Fly    49 -QTAGSPRPPGLEHCVDQEERRVARRLLYISTLDGRLSALDIAKSGKLRWSVPTGPGPLISSSIH 112
             ..||.|.....|...|.|.|...|.|:.|||||||::|||....||.:|.:..|.|.|:|||:.
Mouse    75 PAAAGEPESRATEPDDDVELRPRGRSLVIISTLDGRIAALDAENDGKKQWDLDVGSGSLVSSSLS 139

  Fly   113 RLELTNNGQFVRMIPSLSGGIYKFDGD--SIDPIPITAEHLLSSSAKFSDDLVISGGKETRSYGV 175
            :.|:..|..   :||||.|.::::|.|  |::.:|.|.|.||.||.||.||:|:.|||...:||:
Mouse   140 KPEVFGNKM---IIPSLDGDLFQWDRDRESMEAVPFTVESLLESSYKFGDDVVLVGGKSLTTYGL 201

  Fly   176 SVRTGQLLYECSLNGCVNSTEEGLAIDDTIREPDEEDQLEDGEQLRDEAGYIVRHDPLLDDVIIV 240
            |..:|:|.|.||..||              |..| .|::|:.|                 |::::
Mouse   202 SAYSGKLRYICSALGC--------------RRWD-SDEMEEEE-----------------DILLL 234

  Fly   241 RRQTQTVRAVESRTGVERWNFSVGQHELDLVRPSECQ-------------------LQPRDELEL 286
            :|..:|||||..|:|.|:||||||..||..:...|.:                   :...:|.|.
Mouse   235 QRTQKTVRAVGPRSGSEKWNFSVGHFELRYIPDMETRAGFIESTFKPGGNKEDSKIISDVEEQEA 299

  Fly   287 AVLDVDIKVVVPEGIICAFSKSEPQTMLWKYKFDHPIVSAWNTNADDELQPIDLFSSAQWLWDQ- 350
            .:||..|||.|.:..:.|||:...: :.|:|:|..||.|||... |.::.||.||....:...:
Mouse   300 TMLDTVIKVSVADWKVMAFSRKGGR-LEWEYQFCTPIASAWLVR-DGKVIPISLFDDTSYTASEE 362

  Fly   351 ---DENDTELPNAPQSPPSIYLGMYDKQLYIQESIRLRQEIMDQTKVYQQLTGDTSL--MPRIPW 410
               ||.|........:..|:|||||..|||:|.|:|:.::.....|..:.:.|:.::  :|.|.|
Mouse   363 ALGDEEDIVEAARGATENSVYLGMYRGQLYLQSSVRVSEKFPTSPKALESVNGENAIIPLPTIKW 427

  Fly   411 KP-ISASSKSLVIFRKDQEDPEMIAEGAVAQGGELVPYDDENFAVAAQSVLNASEFVNGNGFYFY 474
            || |.:.|::.|:...|:.|..:..:          .|..|.::..|.|:|   ::...||:|.
Mouse   428 KPLIHSPSRTPVLVGSDEFDKCLSND----------KYSHEEYSNGALSIL---QYPYDNGYYL- 478

  Fly   475 TTGDLNGPQECSTQNNPTDLPAITAPTSPTNATSEGTEATGNHSVNDDLGFSLDDIDAP------ 533
                                     |..........|:.|            :..:|:|
Mouse   479 -------------------------PYYKRERNKRSTQIT------------VRFLDSPHYSKNI 506

  Fly   534 -VKVVILSLWFWWKEI-----VVIAFTSAVILNIFMGQ-RNQRVEREYLVIERHVPVQTAIEATE 591
             .|..|| |..|||||     :.|..|:.::..:|..| ..||.|.|                |:
Mouse   507 RKKDPIL-LLHWWKEIFGTILLCIVATTFIVRRLFHPQPHRQRKESE----------------TQ 554

  Fly   592 ASTQALLGPVVPMQRPGNRFSFPPGQANQRTISEST----THSGEHYTSRFQSDFELMQCLGRGG 652
            ..|::....|                  ...:|:::    .:||  |.||:.:|||.:||:||||
Mouse   555 CQTESKYDSV------------------SADVSDNSWNDMKYSG--YVSRYLTDFEPIQCMGRGG 599

  Fly   653 FGVVFEAKNKLDENRYAIKRITLPNKESSRQRVLREARTLASCEHHNIVRYFHSWTETPPTGWQE 717
            ||||||||||:|:..||||||.|||:|.:|::|:||.:.||..||..|||||::|.||||..|||
Mouse   600 FGVVFEAKNKVDDCNYAIKRIRLPNRELAREKVMREVKALAKLEHPGIVRYFNAWLETPPEKWQE 664

  Fly   718 EEDRKLLAHELSTSIQIETPDDSTMPSL----TEQLKEKRQQQLLSWVSDAANSTACSHDFHLP- 777
            |.|...|..| ||...:.:|.....||:    .:....|.|.:::      |.|...|..|.:.
Mouse   665 EMDEIWLKDE-STDWPLSSPSPMDAPSVKIRRMDPFSTKEQIEVI------APSPERSRSFSVGI 722

  Fly   778 --GESSLKNIREEYDYDEEEDSLIEF-------RSESQSAALRAEEEDDTDDDYEEDEEQQGDHE 833
              |::|         ..|.:.|.:||       .|:|..||...::...||.:..||....|:.|
Mouse   723 SCGQTS---------SSESQFSPLEFSGTDCGDNSDSADAAYNLQDSCLTDCEDVEDGTVDGNDE 778

  Fly   834 KRHRSSVSIDIHSASFDL---KNINYSQHQLVSNSFQIESVRPKSSGSDDA-----------NDD 884
                        ..||:|   :...|::.:..::|    |:..:.||..:|           :|.
Mouse   779 ------------GHSFELCPSEASPYTRSREGTSS----SIVFEDSGCGNASSKEEPRGNRLHDG 827

  Fly   885 NKARRKPLTLALAQNHNNNQ------NGSQPTPSSATILNGTVA--KPS--KVYLYIQMQLCRKE 939
            |....|...|..:.:.::::      :.::||..|......||.  :||  ||||||||||||||
Mouse   828 NHYVNKLTDLKCSSSRSSSEATTLSTSPTRPTTLSLDFTKNTVGQLQPSSPKVYLYIQMQLCRKE 892

  Fly   940 SLRDWLR-----DNRSETRAAHIGDIFHQIVDAVDYVHLKGLIHRDLKPSNIFFSQDGQIKIGDF 999
            :|:||:.     ::|......|   ||.||.:||:::|.|||:|||||||||||:.|..:|:|||
Mouse   893 NLKDWMNRRCSLEDREHGVCLH---IFLQIAEAVEFLHSKGLMHRDLKPSNIFFTMDDVVKVGDF 954

  Fly  1000 GLVTDMADIPNLVAKCGDQ--------SGLPSCARHTQQVGTHLYMSPEQLLGQHYDYKVDIYSL 1056
            ||||.|           ||        :.:|:.|.||.||||.|||||||:.|.:|.:||||:||
Mouse   955 GLVTAM-----------DQDEEEQTVLTPMPAYATHTGQVGTKLYMSPEQIHGNNYSHKVDIFSL 1008

  Fly  1057 GLIFFELHVYFSTEMERIKTLRSLRDGQYPKDFAVNYPQQYDLLQQMLSAQPEQRPQTKQLKSQL 1121
            |||.|||...|||:|||::.|..:|:.::|..|...|||::.::|.|||..|.:||:...:   :
Mouse  1009 GLILFELLYPFSTQMERVRILTDVRNLKFPLLFTQKYPQEHMMVQDMLSPSPTERPEATDI---I 1070

  Fly  1122 RNILQLPHLLSEGQSEQAELAERARRLSRSRTFSSSSEPH 1161
            .|.:          .|..|...:.....|||:.|||...|
Mouse  1071 ENAI----------FENLEFPGKTVLRQRSRSMSSSGTKH 1100

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
PEK	NP_649538.1	Luminal_EIF2AK3	73..401	CDD:188874	119/352 (34%)
		PKc_like	635..1113	CDD:304357	204/528 (39%)
		S_TKc	<925..1118	CDD:214567	102/207 (49%)
Eif2ak3	NP_034251.2	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	72..92		5/19 (26%)
		Luminal_EIF2AK3	100..416	CDD:188874	119/352 (34%)
		PKc_like	583..>671	CDD:304357	55/87 (63%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	775..827		11/67 (16%)
		STKc_EIF2AK3_PERK	<874..1073	CDD:270950	103/215 (48%)
		S_TKc	<879..1071	CDD:214567	101/208 (49%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1087..1114		7/14 (50%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	202	1.000	Domainoid score	I2989
eggNOG	1	0.900	-	-		E1_KOG1033
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	485	1.000	Inparanoid score	I1424
Isobase	1	0.950	-	0	Normalized mean entropy	S5352
OMA	1	1.010	-	-		QHG49379
OrthoDB	1	1.010	-	-		D433474at33208
OrthoFinder	1	1.000	-	-		FOG0005413
OrthoInspector	1	1.000	-	-		oto95374
orthoMCL	1	0.900	-	-		OOG6_106778
Panther	1	1.100	-	-	O	PTHR11042
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R4549
SonicParanoid	1	1.000	-	-		X3837
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
	16	15.820