Protein Alignment cno and afdn

DIOPT Version :10

• Sequence 1: NP_524232.2
• Gene: cno / 40620
• FlyBaseID: FBgn0259212
• Length: 2051
• Species: Drosophila melanogaster
• Sequence 2: XP_031758152.1
• Gene: afdn / 100493083
• XenbaseID: XB-GENE-948333
• Length: 1902
• Species: Xenopus tropicalis
• Alignment Length: 2193
• Identity: 728/2193 - (33%)
• Similarity: 1068/2193 - (48%)
• Gaps: 457/2193 - (20%)

  Fly     9 DREAVRSVIQQWNANRLDLFALSEPDENLLFHGVMRFYFQD-AGQKVATKCIRVASDATVTDVID 72
            :|..:..:|..|||||||||.:|:|.|:|.||||||||||| |....|||||||:|.||..|||:
 Frog     8 ERRKLADIINHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIE 72

  Fly    73 TLIEKFRPDMRMLSVPNYALYEVHANGEERRLNADEKPLLVQLNWHIDDREGRFLLKNID----- 132
            ||.|||||||||||.|.|:|||||.:||||:|:.:||||:|||||:.|||||||:|||.:
 Frog    73 TLAEKFRPDMRMLSSPRYSLYEVHVSGEERKLDFEEKPLVVQLNWNKDDREGRFVLKNENDIFPA 137

  Fly   133 ---QKTTPIEQTD----LNFKRKLSKREKKEQKKKEK-MAKLSSDPPTSNGSHLGLGNGVGASSG 189
               |...|.:|..    .||||.|||:||||:|::|: :.|.|:|
 Frog   138 KKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKRRERDILKQSTD-------------------- 182

  Fly   190 SAHMNGNAGGTGGDSSD-AVAGKLYTELPETSFTRSISNPEAVMRRRRQQKLEKKLQQFRSRDGG 253
                |.:......||.: .:|.::|.::|||||||:|||||.||:|||||||||::|:|||.||.
 Frog   183 ----NEDRPQVRDDSENLQLAMEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGR 243

  Fly   254 PDTGGTLKIYGESLCQDVPYKTLLLSIRDCAQAVVREMLTKYGLEKADPLHYCLVQV-------N 311
            ||:||||:||.:||..::||||:|||..|.|...|.|.|.|||:||.:|..||:.:|       :
 Frog   244 PDSGGTLRIYADSLKPNIPYKTILLSTNDTADFAVIEALEKYGMEKENPKEYCIAKVVLPPGAQH 308

  Fly   312 SDG--TEYILDDDECPLSILMNHPTSRGSIMFHVRRRPADS---QPRRRKKKPLGAANGANH--- 368
            ||.  .|:||||:||||.|..:.|:.:|.::|.::|||.||   |.|:.:.|...:....:|
 Frog   309 SDKGMKEFILDDEECPLHIFRDWPSEKGELVFQLKRRPTDSLLKQNRKHESKSGKSKERGDHYSL 373

  Fly   369 -ISGDREGPVLVEVTHSG----DGGRRIKLGSDPVEVGSA--NTNCLQLFGPSIQPRHCLISLLE 426
             :|.|:. |.|||::..|    |..:..:|.....|||:.  ..|.:|||||.||..||.::.::
 Frog   374 SLSPDKL-PYLVELSADGSDSRDKPKLYRLQPSVTEVGTEKFQDNSIQLFGPGIQHHHCDLNNMD 437

  Fly   427 GVCTVTPLHTDALTFVNGHHISQPTILHNGSVVMFGRVASYRFLDSPTDGRYNLALSQSQLDSAC 491
            ||.||||...||.|:|:||.||:.|:||:|..|.||....::|:|.      ||
 Frog   438 GVVTVTPRSIDADTYVDGHRISETTMLHSGVTVQFGSSHVFKFVDP------NL----------- 485

  Fly   492 LYESRSPTSPGSWNDEDGALSSTHKSDYDHHQQANHSSAYHNNNNNSSDHPLSSTLRDVVDSKAG 556
                                                            ||.|...|.|.  .:.|
 Frog   486 ------------------------------------------------DHLLGKRLSDA--GQIG 500

  Fly   557 QDQGANSGNYDGQ-SLEGNLENE-----TKSISSLKSGGSNSQDRSPKMTGSGILAG-------- 607
            ::|.:..|..:.. .|||.:..:     .|.:|.|      ..||:|.||..|::..
 Frog   501 KNQRSKQGLQETTFGLEGEVLGDVTLPVNKMVSKL------DNDRTPGMTERGMVKPIIRMDKQQ 559

  Fly   608 ----GGSGSGDTQGQEPILPAVLEFPETHQELFLRHIISELDVNVPHFKLAPVYSLYLCARYRAS 668
                ....|.|:||.|.:|||.:||.|..::.||..||:..:.:..||||:|.|.||:..||..|
 Frog   560 DQRVQNGRSQDSQGPENVLPANIEFRENSEDAFLSAIINYTNSSTVHFKLSPTYVLYMTCRYVLS 624

  Fly   669 THYRPELQPTERAHKLTMFLHHVANLVYSVVQE-------QYTDPRILAFWMANSSEFLHFLKSD 726
            ..|||:..|.||.||:...::.:..::.||:||       |......|||||||:||.|:|:|.|
 Frog   625 NKYRPDTSPAERTHKVIAVVNKMVGMMESVIQEIDKGNQKQKNIAGALAFWMANASELLNFIKQD 689

  Fly   727 RHISAFSVQAQEVLAEAVQTAFRNLVNCFRLELSQTLNQFLSENIDHDSAAGL---VLTVLGSAM 788
            |.::..::.||:|||..||.||:.||:|.:.||:.::..|:.:..::||....   ||..|..||
 Frog   690 RDLNRITLDAQDVLAHLVQMAFKYLVHCLQSELTSSMPAFIDDPEENDSQRPKIEDVLHTLTGAM 754

  Fly   789 ALLRRCRVNAALTIQLFSQLFHYINVICFNTIVA--NSQMCTGDWGKVMTERLQLLELWAERQGL 851
            :|||||||||||||||||||||:||:..||.:|.  :|.:|:..||.::.::|..:|.|||:|||
 Frog   755 SLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLAHIEAWAEKQGL 819

  Fly   852 ELAADCHLAKINQCAQFLQAPKSSVEEIQQLACSCFRLNSLQMAALLQ-------QEKLPRNLVD 909
            ||||||||::|.|....|...|.|.::|..:..:||:|||||:.||||       :..:|..|:|
 Frog   820 ELAADCHLSRIVQATTLLTMDKYSHQDIPNINNTCFKLNSLQLQALLQNYHCAPDEPYIPTELID 884

  Fly   910 TAIRMAESVADELTRADGREVRLEESPELHLALLLPDDGFSCDVVRGIPTGLVDFLNPLQQQGMC 974
            :.:.:||:.||||.|:|||||:|||.|:|.|..|||:||:||||||.||.||.:||.||.|:|.|
 Frog   885 SVVSVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLEPLCQRGFC 949

  Fly   975 RLAAQPTSIGLWTVYMHQFNARSSSAMSN-KLP---QPELQLIKLHKNSNGMGLSIVAAKGAGQE 1035
            ||.....|.|:||:|.......::....| .||   :||:..:.| |..||||||||||||||||
 Frog   950 RLVPHSRSPGMWTIYFEGAEFENNLLTDNTDLPLRKEPEIITVTL-KKQNGMGLSIVAAKGAGQE 1013

  Fly  1036 KLGIYIKSVVPGGAADADGRLQAGDQLLRVDGQSLIGITQERAADYLVRTGPVVSLEVAKQGAIY 1100
            ||||||||||.||||:.||||.|||||:.||||||:|::|||||:.:.|||.||:||||||||||
 Frog  1014 KLGIYIKSVVKGGAAEVDGRLAAGDQLISVDGQSLVGLSQERAAELMTRTGAVVTLEVAKQGAIY 1078

  Fly  1101 HGLATLLQQPSPVIQRGNRRMSERDLTRMGGAESTKSLGPLIANNHPNHSLSLSQHLNNSHNNTL 1165
            |||||||.||||::|| :||.|        |....||.|..:.||
 Frog  1079 HGLATLLNQPSPLMQR-DRRGS--------GKPRPKSEGFELYNN-------------------- 1114

  Fly  1166 GNSNNSSIINPLA--AHLPNSKSVPALHHHTGSGTISLANSKSRSTHSLHNNTSGMGGIGGAAAG 1228
             .|.|.|..:|..  ......|.:|      |...:....:..||:.::.|...        :.|
 Frog  1115 -ASQNGSPDSPQTQWPEYTEPKKLP------GDDKLMKNRADHRSSPNVANQPQ--------SPG 1164

  Fly  1229 GMLGQPNGSQNNANGNGNGNEQGFYQNLSVYRAQNQSQPILNERPPIAAHAAMNAY------NGS 1287
            |.....:.|.........||          ...:.|:.|...|..||........|      ...
 Frog  1165 GKTTYSSSSTVKITSVSTGN----------LCTEEQTAPARPEAYPIPTQTYAREYFTFPTPKSQ 1219

  Fly  1288 SPLAPQQQPQQQQQSPYQQQQQQQHMQANANLPPTRPVSAYYHSQQSAQQQLQQQQQQQQQHSLQ 1352
            ..:.|.|...|..:..:|.|....|.:                 ..||||....:::.:...:.|
 Frog  1220 DRIGPAQNQWQGYEDNFQVQMGNNHAR-----------------NTSAQQVAYSEEELRDNLTFQ 1267

  Fly  1353 QQQFALSSGNLNGQQQQQQQHQHQHQLTLNNRTKSQQNFQHTLRMQQMMAPSMPNISNM------ 1411
            .::     |.:..|.::..:|...:. |..||..|.::......:......|:.::|..
 Frog  1268 SER-----GRVEPQVRKDVEHLDGND-TWFNRGSSMESSASCQDLNNSKTHSVGSLSKTGPGRWK 1326

  Fly  1412 ---------------------------YHHQQQQQQ---QLPLQQQQQQQQQPLMSSSQSMQNVN 1446
                                       .|:..:.:.   .|||.....:.|..|.::||:
 Frog  1327 TPISSQSATVLLPQPVRTDLPPPPPPPVHYGAEFEGVPIDLPLPPPPSESQLGLSTNSQA----- 1386

  Fly  1447 DFAGGYQNGSLEYRRSQLHDPSTLYEIQQQQLQQQQQQQQQQQQQQ--QASPNFIALPPKPLGSL 1509
                    .:||.::.:.|  ...||.::.:|::::::::::|:::  |..|....:||:|:..
 Frog  1387 --------AALERKKREEH--QRWYEKEKARLEEERERKRREQERKLGQVRPQPPTIPPQPVNP- 1440

  Fly  1510 QSPNKPNVPPSTAPKPQQDKPPLPPTATHPLFKATQ-QIAPGMNYVASTLDPPKGSYVASNQGNN 1573
            |||  |.:||....:.:.:||...|.....:.:..| |..|      .|::.....|:..::...
 Frog  1441 QSP--PLIPPPNVAQARTEKPATLPRQQETVIRELQPQQQP------RTIERRDLQYITVSKEEL 1497

  Fly  1574 RPLHSGS-NPWEREEREKDLEMRREHIRQWREQQISELSQIVSRSPMQEEQLKTLILERDFERRA 1637
            ....|.| :||:|:.:||..:.::.||.....::|:||.....|:..:.::|:.|:||..|::|.
 Frog  1498 SSSDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEINELQNKPDRTSEENDRLRKLMLEWQFQKRL 1562

  Fly  1638 QELQEQEEQDQEQQYDKENVQELF---RLAGGGQVSAIQTPITSYRQTEIKLAEMPDSNSLVDSV 1699
            ||.|::::.|.:...|.::|..:.   ||....:.          |||     .|| :.|::|.:
 Frog  1563 QESQQKDDDDDDDDDDDDDVDTMLIMQRLEAERKA----------RQT-----AMP-AISILDLL 1611

  Fly  1700 PPQPPAPTAQPLSSNTQQPKSILK---HNRYSEGGVGPSGAPSSPSKSQKSASFADERHLHTEHP 1761
            ..:..........:..::.:..|:   .:|:.|              .:::...|:|:....|..
 Frog  1612 QDEERRRQQHLEEARKREAEDRLRQEEEHRHQE--------------EERARREAEEKRRQEEEY 1662

  Fly  1762 ISNLAKELNQL------TMLDKDN--------------NNETLDAVVPPPPPPERNSSYLIMSQQ 1806
            .:.|..|..::      .:||.:.              ::.||.. .||||||:||:||
 Frog  1663 YNRLEAERRRIHEEAERRLLDPEEPGLYRPPLPREYELSSPTLPP-NPPPPPPQRNTSY------ 1720

  Fly  1807 KLRGSTGNATSSMGLLKTATSNQAAAAVEIKKASLLN-TQTNNNNNLSGSLNNNTMHGSPLSAME 1870
                           |||    ||.:...:..|..:. |....:.||||....::|..|
 Frog  1721 ---------------LKT----QALSPDTVYTAKFVTYTDDEEDTNLSGQDKYSSMRKS------ 1760

  Fly  1871 LNAAYVSATGTGIIGGLGTPPPPPPLMQRDNKRVSFHDEENNFVSGNSQQQQLQQQYIMDNYGME 1935
                            .|..||.....|....|:.....:..|:||:.|.............|
 Frog  1761 ----------------YGDLPPAAHKPQLPAARLPRPVSDGIFLSGSFQPPSANANSTASKTG-- 1807

  Fly  1936 HQDLDTIREDTSYLQHNLDASMLPSMPATPDAALWNTSVQSTPGVIGAQEVYRDPRTRRLAEKQQ 2000
                ..|....|:|.::  :|..|           |:...|:...:...|||:|||..||  |.|
 Frog  1808 ----PPIANKPSFLHYS--SSKGP-----------NSYSGSSGAAVPGNEVYKDPRDVRL--KSQ 1853

  Fly  2001 QQQQQRAGDAVPEKLSFKEKMKMFALESGEDKTPKDKLKISRAQRDID 2048
            ..:....|.| ||.|:|||:.::|:  .|:|.:  :|:|.||...:::
 Frog  1854 DGEAYSTGGA-PESLTFKERQRLFS--QGQDVS--NKVKASRKLMELE 1896

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
cno	NP_524232.2	RA1_Afadin	20..130	CDD:340480	80/110 (73%)
		RA2_Afadin	257..347	CDD:340479	45/98 (46%)
		FHA	376..471	CDD:469597	42/100 (42%)
		Myo5p-like_CBD_afadin	631..941	CDD:271255	147/328 (45%)
		PDZ_AFDN-like	1008..1096	CDD:467251	64/87 (74%)
afdn	XP_031758152.1	RA1_Afadin	19..131	CDD:340480	81/111 (73%)
		RA2_Afadin	247..346	CDD:340479	45/98 (46%)
		FHA_AFDN	380..483	CDD:438763	42/103 (41%)
		Myo5p-like_CBD_afadin	587..916	CDD:271255	147/328 (45%)
		PDZ_AFDN-like	987..1074	CDD:467251	64/87 (74%)