DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Gnf1 and RFC1

DIOPT Version :9

Sequence 1:	NP_001014605.1	Gene:	Gnf1 / 40607	FlyBaseID:	FBgn0004913	Length:	1008	Species:	Drosophila melanogaster
Sequence 2:	NP_014860.1	Gene:	RFC1 / 854392	SGDID:	S000005743	Length:	861	Species:	Saccharomyces cerevisiae

Alignment Length:	970	Identity:	311/970 - (32%)
Similarity:	474/970 - (48%)	Gaps:	176/970 - (18%)

- Green bases have known domain annotations that are detailed below.

  Fly    94 VDPTELFGGETK--RVIVPKPKTKAVLEFENEDIDRSLMEVDLDESIKEAAPEK-KVHSI----- 150
            |:.::.||...|  |....:| |:.|...:.|.||   ::.:.|:......|:| .|.::
Yeast     2 VNISDFFGKNKKSVRSSTSRP-TRQVGSSKPEVID---LDTESDQESTNKTPKKMPVSNVIDVSE 62

  Fly   151 ----TRSSPSPKRAKNSSPEPPKPKSTKSKATTPRVKKEKPAADLESSVLTDE-------ERHER 204
                .:..|.|.:.|.||| ..||.|  ||.|.|..|....|:::.:..:.|:       ..|.:
Yeast    63 TPEGEKKLPLPAKRKASSP-TVKPAS--SKKTKPSSKSSDSASNITAQDVLDKIPSLDLSNVHVK 124

  Fly   205 KRASAVLYQKYKNRSSCLNPGSKEI-------PKGSPDCLSGLTFVVTGVLESMEREEAESVIKE 262
            :.|      |:..:|:..|....||       |:|.|:||.|||.|.||||.::||..:|::.|.
Yeast   125 ENA------KFDFKSANSNADPDEIVSEIGSFPEGKPNCLLGLTIVFTGVLPTLERGASEALAKR 183

  Fly   263 YGGKVMTVVGKKLKYLVVGEEAGPKKLAVAEELNIPILSEDGLFDLIR---EKSGIAKQVKEEKK 324
            ||.:|...:..|...:|:|:|||||||...::|.|..:.|:|...||.   .:.|..:..::.::
Yeast   184 YGARVTKSISSKTSVVVLGDEAGPKKLEKIKQLKIKAIDEEGFKQLIAGMPAEGGDGEAAEKARR 248

  Fly   325 SPKKEHSSEEKGKKEVKTSRRSSDKKEKEATKLKYG--EKHDIAKHKVKEEHTSPKETKDKLNDV 387
            ..:::|:...| :.|:...:.....|:..||::..|  |:.::    |:||        |||
Yeast   249 KLEEQHNIATK-EAELLVKKEEERSKKLAATRVSGGHLERDNV----VREE--------DKL--- 297

  Fly   388 PAVTLKVKKEPSSQKEHPPSPRTADLKTLDVVGMAWVDKHKPTSIKEIVGQAGAASNVTNHNLKL 452
                                               |..|:.||:::::.|..|:.       :||
Yeast   298 -----------------------------------WTVKYAPTNLQQVCGNKGSV-------MKL 320

  Fly   453 KAKQERVKVLHYFNFPRLMNWLSKWYVNHDGNKKPQRPNPWAKNDDGSFYKAALLSGPPGIGKTT 517
            |                  |||:.|    :.:||....:  |..|....::||:|.|||||||||
Yeast   321 K------------------NWLANW----ENSKKNSFKH--AGKDGSGVFRAAMLYGPPGIGKTT 361

  Fly   518 TATLVVKELGFDAVEFNASDTRSKRLLKDEVSTLLSNKSLSGYF--TGQGQAVSRKH-VLIMDEV 579
            .|.||.:|||:|.:|.||||.|||.||...|...|.|.|:.|||  ..:.|.::.|| |:|||||
Yeast   362 AAHLVAQELGYDILEQNASDVRSKTLLNAGVKNALDNMSVVGYFKHNEEAQNLNGKHFVIIMDEV 426

  Fly   580 DGMAGNEDRGGMQELIALIKDSSIPIICMCNDRNHPKIRSLVNYCYDLRFQRPRLEQIKGKIMSI 644
            |||:|. ||||:.:|....:.:|.|:|.:||:||.||:|.....|.|::|:||....||.::|:|
Yeast   427 DGMSGG-DRGGVGQLAQFCRKTSTPLILICNERNLPKMRPFDRVCLDIQFRRPDANSIKSRLMTI 490

  Fly   645 CFKEKVKISPAKVEEIIAATNNDIRQSINHIALLSA------KEDASQKSGQQVATKDLKLGPWE 703
            ..:||.|:.|..::.:|..|..||||.||.::.:|.      .|:.::.|  :...|::.|.|::
Yeast   491 AIREKFKLDPNVIDRLIQTTRGDIRQVINLLSTISTTTKTINHENINEIS--KAWEKNIALKPFD 553

  Fly   704 VVRK-----VFTADEHKHMSFADKSDLFFHDYSLAPLFVQQNYLQVLPQGNK--KDVLAKVAATA 761
            :..|     :::....::.:..||..|:|.|:...||.:|:|||...|...|  :..|..||..|
Yeast   554 IAHKMLDGQIYSDIGSRNFTLNDKIALYFDDFDFTPLMIQENYLSTRPSVLKPGQSHLEAVAEAA 618

  Fly   762 DALSLGDLVEKRIRAN-SAWSLLPTQAFFSSVLPGEHMCGHFTGQINFPGWLGKNSKSGKRARLA 825
            :.:||||:|||:||:: ..|||||..|..|||.|...:.||..|:|||..|||:||||.|..||.
Yeast   619 NCISLGDIVEKKIRSSEQLWSLLPLHAVLSSVYPASKVAGHMAGRINFTAWLGQNSKSAKYYRLL 683

  Fly   826 QELHDHTRVCTSGSRLSVRLDYAPFLLDNIVRPLAKDGQEGVPAALDVMKDYHLLREDLDSLVEL 890
            ||:|.|||:.||..::.:||||.|.....::.|..|.|.:.:.:.::||.||:|.:||.||::|.
Yeast   684 QEIHYHTRLGTSTDKIGLRLDYLPTFRKRLLDPFLKQGADAISSVIEVMDDYYLTKEDWDSIMEF 748

  Fly   891 TSWPG------KKSPLDAVDGRVKAALTRSYNK---EVMAY---SYSAQAGIKKKKSEAAGADDD 943
            ...|.      ||.|     ..||:..||.||.   .|..|   |.....|:....|.....|..
Yeast   749 FVGPDVTTAIIKKIP-----ATVKSGFTRKYNSMTHPVAIYRTGSTIGGGGVGTSTSTPDFEDVV 808

  Fly   944 YLDEGPGEEDGAGGHLSSEEDEDKD-NLELDSLIK-----AKKRTTTSKASGGSKKATSS 997
            ..|:.|...|       .||.:|.. :|:.|.|||     .|::|.|||..|..|:.|.:
Yeast   809 DADDNPVPAD-------DEETQDSSTDLKKDKLIKQKAKPTKRKTATSKPGGSKKRKTKA 861

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Gnf1	NP_001014605.1	COG5275	<151..358	CDD:227600	66/223 (30%)
		BRCT	232..309	CDD:278934	33/76 (43%)
		PRK04195	421..969	CDD:235250	209/577 (36%)
		AAA	506..633	CDD:278434	66/129 (51%)
		RFC1	764..917	CDD:285691	70/159 (44%)
RFC1	NP_014860.1	COG5275	1..277	CDD:227600	79/288 (27%)
		PRK04195	298..762	CDD:235250	189/497 (38%)
		RFC1	621..776	CDD:400703	70/159 (44%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	136	1.000	Domainoid score	I1078
eggNOG	1	0.900	-	-		E1_COG5275
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H2187
Inparanoid	1	1.050	422	1.000	Inparanoid score	I284
Isobase	1	0.950	-	0	Normalized mean entropy	S1907
OMA	1	1.010	-	-		QHG52880
OrthoFinder	1	1.000	-	-		FOG0003699
OrthoInspector	1	1.000	-	-		oto100300
orthoMCL	1	0.900	-	-		OOG6_101059
Panther	1	1.100	-	-	LDO	PTHR23389
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R1087
SonicParanoid	1	1.000	-	-		X3601
TreeFam	1	0.960	-	-
	15	14.810