DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment smash and RGA1

DIOPT Version :9

Sequence 1:	NP_001246912.1	Gene:	smash / 40583	FlyBaseID:	FBgn0263346	Length:	1541	Species:	Drosophila melanogaster
Sequence 2:	NP_014770.1	Gene:	RGA1 / 854294	SGDID:	S000005653	Length:	1007	Species:	Saccharomyces cerevisiae

Alignment Length:	1019	Identity:	182/1019 - (17%)
Similarity:	328/1019 - (32%)	Gaps:	341/1019 - (33%)

- Green bases have known domain annotations that are detailed below.

  Fly   120 EHIID-RAVETKK--LELEEHDRLRRKSKTFTEMMEERAERGGPRGRAKLASL--AVYNEDETND 179
            |:|.| |..:||:  ..|..|::|..|.|.:.|...        |.:..|.||  .|.:...|::
Yeast   103 ENIADLRYAKTKRGLFCLSCHEKLLAKRKYYEEKKR--------RLKKNLPSLPTPVIDNGHTDE 159

  Fly   180 LSDLGI-GTSSASGKSSLSGDYDNNSVMSDHAAELDKAIGAASS--VAAGVTAQNVDEQLNHINR 241
            :|...: ...:.|..:||..:..:.|       |..|.|...||  |...:|..|..:.
Yeast   160 VSASAVLPEKTFSRPASLVNEIPSGS-------EPSKDIETNSSDIVPHFITGYNDSDD------ 211

  Fly   242 NGSNGNHGNGAAVGQTGPSNSSKSAGRQYISSPGYDTSSSNAQASSPDLCEYTYEGAIQDYKQRV 306
            |..:...|:..::...||..:|    .::::    |.....|:|.|.::............|:..
Yeast   212 NSGSSKFGSNVSIDVIGPEENS----TEHVN----DDVKEEAEAPSANMSLNVATDPTLSCKEPP 268

  Fly   307 QRASSNGNGNANGKLIGEHIAYPTRRGSKIEDRLSGFEVTSPSDTQEGV-------EKQKVDVPK 364
            ..:.:..|........|:::|                  .|||..::|:       |..::|.|.
Yeast   269 SHSRNLLNKTPLRNSSGQYLA------------------KSPSSYRQGIIVNDSLEESDQIDPPN 315

  Fly   365 VDISKRKEIF-----EQAKAEVSNGGAPAAPKLVFRDGLTNGNSATTAPKSEVRRLSGDISSIRD 424
            .......|:.     ......:.|      ||         |::.......|:.::...:|
Yeast   316 NSSRNASELLTSVLHSPVSVNMKN------PK---------GSNTDIFNTGEISQMDPSLS---- 361

  Fly   425 RMQSLEQQRKAFSSSKSVDVPVPPLKQRLNSLQQ---SVAKEEQKKPPLVALIDARQLEIMRGEE 486
                    ||..::          :.:..|:||:   .|.||::..|.|..:
Yeast   362 --------RKVLNN----------IVEETNALQRPVVEVVKEDRSVPDLAGV------------- 395

  Fly   487 ERMRQQQQKEKHSPSQTTLCAPKPA------PPALIIEEPPVADTNDDSGIQEDTADEL--QQQQ 543
                ||:|.||:|.|..:....|.:      ...|:|.....|....||.::...|.::  ::.|
Yeast   396 ----QQEQAEKYSYSNNSGKGRKISRSLSRRSKDLMINLKSRATGKQDSNVKLSPASKVTSRRSQ 456

  Fly   544 QL-------------------------------------NAAIAALALEERQLEEAANAVNQIEA 571
            .|                                     |..:...:.:|..|||..|
Yeast   457 DLMRDNDSHTGLDTPNSNSTSLDILVNNQKSLNYKRFTDNGTLRVTSGKETALEEQKN------- 514

  Fly   572 EFDELTDLH----PSP---LPPSPALPAVQQSLQQPTVAQVPAQAVNQAAAPPLRDMEFSINEAL 629
                    |    |||   |..|||.|:                .|:....|||
Yeast   515 --------HSFKSPSPIDHLLQSPATPS----------------NVSMYRTPPL----------- 544

  Fly   630 ELALQAIDREATTKPMDKAQHPQKREQKEELGGKPE-EKEPICEEQHDEESNEQGETHNEGGEMP 693
                   |...|   .|:........|...:   |. :|.|..:.::.:...||.||..|..|..
Yeast   545 -------DSSLT---FDRRNGSSYSNQNYSI---PSWQKTPKTQLENSDNFEEQKETLYENSESR 596

  Fly   694 N--KTDKQMQGGREPIYENVSSTISMHEVDNEQIATMTITTQTQATRISHRRSIPSQQTSNSNEN 756
            |  ..||::......:.:   ..|::.|:::::...|...|:.::.:.:.||.|.| ..|..|:.
Yeast   597 NDPSLDKEIVTAEHYLKQ---LKINLKELESQREELMKEITEMKSMKEALRRHIES-YNSEKNKL 657

  Fly   757 NQSTNQTSN------------SRNQKESSTAATSAANPVPPTTPGEQAEPYYQVPKATEPYYDAP 809
            ...:|:.||            |...|..:||::.|.:.|.|        .:::...:.:|  ...
Yeast   658 YLDSNELSNNPPMINEISLGESPPVKHVATASSVARSSVKP--------KFWKFFSSAKP--QTE 712

  Fly   810 KHLRPVPVYENVEIFYSG-LEISQGSVGAPVGLME--PPKEKPPPP-------PTESPIPLDEGH 864
            :.::.|.......|..|. :.:|..|.|:..|.:|  ||..:.|..       |.|:...:.:..
Yeast   713 QSIQGVSTNNTNSIVKSAPVLLSAPSSGSNSGRLEISPPVLQNPNEFSDVRLVPIENDANMGQSK 777

  Fly   865 DDE--LDG---LGNSL------------------------------------------------- 875
            |.|  |||   .|:||
Yeast   778 DGEEYLDGSNLYGSSLVARCNYENNEIPMILSVCIDFIESDEENMRSEGIYRKSGSQLVIEEIEK 842

  Fly   876 -------GHNTDTWS--SDNTYETISNGTRRHLQGQPEPA---QPSPPIKRMNSTKRIKKELRNK 928
                   ..||:|.:  ::.....::...:|:|:..|.|.   |...|:.|:..:|::.:.|
Yeast   843 QFSAWKVQQNTETPNILTEQDLNVVTGVLKRYLRKLPNPIFTFQIYEPLMRLVKSKKMMENL--- 904

  Fly   929 RSSFLGIENDGDLDDMETYLELTVAPPPDMAQLLQEERRLEKQL 972
              .|:|.:...:..:.:||:....|....:..|.:|..|:.:.|
Yeast   905 --PFVGGKLSLEAKNSDTYMSSKSALKNILEDLPREHYRVLRVL 946

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
smash	NP_001246912.1	DUF4757	14..152	CDD:292571	12/34 (35%)
smash	NP_001246912.1	LIM	<1494..1530	CDD:278823
RGA1	NP_014770.1	LIM1_Rga	13..67	CDD:188780
		LIM2_Rga	70..123	CDD:188781	7/19 (37%)
		Smc	<569..>673	CDD:224117	24/107 (22%)
		RhoGAP	803..1002	CDD:214618	21/149 (14%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG1704
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	0	0.000	Not matched by this tool.
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	1	0.900