Protein Alignment smash and Limch1

DIOPT Version :10

• Sequence 1: NP_001246912.1
• Gene: smash / 40583
• FlyBaseID: FBgn0263346
• Length: 1541
• Species: Drosophila melanogaster
• Sequence 2: NP_001388345.1
• Gene: Limch1 / 77569
• MGIID: 1924819
• Length: 1537
• Species: Mus musculus
• Alignment Length: 1783
• Identity: 333/1783 - (18%)
• Similarity: 571/1783 - (32%)
• Gaps: 618/1783 - (34%)

  Fly    46 PYVTKQRVTRPTPPAPSKNPMQFVQIKPCNLYQTAQEQLKKAEEVKKLKEVKKEEPEEWQN---- 106
            |.:.|:....|||.|...|.:.|               |:..:|: .|||.:..:|.:.|:
Mouse    63 PGLVKKINRLPTPIAGLDNTILF---------------LRGCKEL-GLKESQLFDPSDLQDTSNR 111

  Fly   107 ----NLDNWKSSRRKRVE-HIIDRAVET------KKLELEEHDRLRRKSKTFTEMMEERAERGGP 160
                |||..:..:...|. :.:.:|..:      ..|.|:|.:.|      ..:|.:|..:...|
Mouse   112 VTVKNLDYSRKLKNVLVTIYWLGKAANSCASYGGTTLNLKEFEGL------LAQMRKETDDIDSP 170

  Fly   161 R--------------GRAKLASLAVYNEDETNDLSDLGIGTSSASGKSS---LSGDYDNNSVMSD 208
            :              .|:...|...:..|::.|..| ..|:.|....|.   |.|..|...  ||
Mouse   171 KRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLD-SFGSRSRQTPSPDVVLRGSSDGRG--SD 232

  Fly   209 HAAELDKAIGAASSVAAGVTAQNVDEQLNHIN-RNGSNGNHGNGAAVGQTGPSNSSKSAGRQYIS 272
            ..::|..             .:..|.:.:.:: |..|:|...:.....|..|:.|:::|   |:.
Mouse   233 SESDLPH-------------RKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTA---YVP 281

  Fly   273 SP-------------GYDTSSSNAQASSPDLCEYTYEGAIQDYKQRVQRAS-----SNGNGNANG 319
            :|             .:.|::|......|    ::|...|::  :.:...|     .:....|:|
Mouse   282 APLRKKKAEREEFRKSWSTATSPLGGERP----FSYPETIEE--EGIDAGSPTEDDPSSQVGADG 340

  Fly   320 KLIGEHIAYPTRRGSKIEDRLSGFEVTSPS---DTQEGVEKQKVDVPKVDISKRKEIFEQAKAEV 381
            |...|..|.|    |..||:..|.....|:   :.::.||.||         :||  .|||..:|
Mouse   341 KPDSELEAPP----SNGEDQSPGDAAVVPAALPEDRDAVESQK---------RRK--LEQAGIKV 390

  Fly   382 SNGGAPAAPKLVFRDGLTNGNSATTAPKSEVRRLSGDISSIRDRMQSLEQQRKAFSSSKSVDVPV 446
                .|||.:.           |:....||.:....||...::......|..|...|.......:
Mouse   391 ----MPAAQRF-----------ASQKQSSEEKEAIRDIVLRKENPFLTHQHGKDSESEDEAACLL 440

  Fly   447 PPLKQRLNSLQQSVAKEEQKKP--PLVALI-------DARQL----EIMRGEEE------RMRQQ 492
            |.|::  :......||..|.||  ||..|:       ||.:.    ::.:|..:      :..|.
Mouse   441 PDLEK--DDFAARRAKMNQSKPMVPLNQLLYGPYRNKDAEKAGGSKQLSKGLSDKKNLGFKRNQG 503

  Fly   493 QQKEKHSPSQTTLCAPKPAPPALIIEEPPVADTNDDSGIQEDTADELQQQQQLNAAIAALALEER 557
            |:::.::....|:...|..|......:.|  |.:.|...|......|...::..:.|....:.|.
Mouse   504 QREDVNTMVACTVQTQKDEPLETNFRKTP--DRHKDDLAQRRIQGRLTPHREAPSFIKLSGITEA 566

  Fly   558 QLEEAAN-AVNQIEAEFDELTDLHPSPLPPSPALPAVQQSLQQPTVAQVPAQAVNQAAAPPLRDM 621
            .||.... .|:....:.|......|.|.|......|::...     |...|:|..:|:.|..:.:
Mouse   567 DLETWERLKVSGETRDGDAQNTCAPEPSPEIDTETAIRDDF-----ASRKARAYKKASGPRQKFV 626

  Fly   622 EFSINEALELALQAIDREATTK--PMDKAQHPQKREQKEELGGKPE-------EKEPICEEQHDE 677
            .|  ....|:..|...|.:..|  |.|..:..:...|.:...|.|.       ::|.:...|.|.
Mouse   627 HF--GPVTEIDQQTWKRLSIGKAGPRDNVEVVRHSSQNQISSGSPPSTATFGLKEEQMLSPQSDC 689

  Fly   678 ESNEQGETHNEGGEMPNKTDKQMQGG--REPIYENVSSTISMHEVDNEQIATMTITTQTQATRIS 740
            .:            :|:.||.:::..  ..|:.|:          ..||:...:...:|:..
Mouse   690 RT------------VPSGTDGRLKKAPWLAPVLES----------QEEQVCIQSNNPKTEEV--- 729

  Fly   741 HRRSIPSQQTSNSNENNQSTNQTSNS---RNQKESSTAATSAANPVPPTTPGEQAEPYYQVPKAT 802
                .|:|...:..|.|:...|..:.   |.|:                 |.|.:.|.|
Mouse   730 ----TPTQLPQDGREQNEEVIQKEHEMLPRVQR-----------------PEECSGPLY------ 767

  Fly   803 EPYYDAPKHLRPVPVYENVE---IFYSGLEISQGSVGAPVGLMEPPKEKPPPPPTESPIPLDEGH 864
                 .|:    .||.::.|   :|....| .:.:|                      :|.....
Mouse   768 -----GPR----TPVSDDAESTSMFDMRCE-EEAAV----------------------LPHSRAR 800

  Fly   865 DDELDGLGNSLGHNTDTWSSDNTYETISNGTRRHLQGQPEPAQPSPPIKRMNSTKR------IKK 923
            .::|..:.|.|....|.|..|                          :.|..|.:|      |||
Mouse   801 QEQLQLINNQLREEDDKWQDD--------------------------LARWKSRRRSASQDLIKK 839

  Fly   924 ELRNKRSSFLGIENDGDLD---DMETYLELTVAPPPDMAQLLQEERRLEKQLYIKAGLCDSSDTG 985
            |...|:...|....||..:   .::||.|           ::||:.|.|::|:      ::....
Mouse   840 EEERKKMEKLMSGEDGTSERRKSIKTYRE-----------IVQEKERRERELH------EAYKNA 887

  Fly   986 ESRDSGVSENHSRQSSEHYT------------------NSSEENDTQSEATPPP--------LPP 1024
            .|::.  :|...:|..|.:|                  :|:|.|.:.....|.|        |||
Mouse   888 RSQEE--AEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNVSSFPNDPSPMKYLRQQSLPP 950

  Fly  1025 PPSTAQVGEVIYQN---ESLLAAQTPLLQTVKGNSAESWTESATAAAAATQSLSEATATANAKMQ 1086
            |..||.|...|.:.   ||:.:|.|       |:.::..|.:...............:....|..
Mouse   951 PKFTATVETTIARTSVPESIASAGT-------GSPSKIITPNTVPMLTPRPYSQPKNSQEVLKTF 1008

  Fly  1087 SIEDKIREQGEMLRVERE--------------------------LLHFSQEELKRQRENL---VL 1122
            .::.|:...|||...:.|                          .:|.|..::|:...::   :.
Mouse  1009 KVDGKVSMNGEMAPGDEEGKEKEGPAAAAPGPSLTKSQMFEGVATVHDSPVQVKQGSNSIEINIK 1073

  Fly  1123 RENIARRELQHGAKMLMSNNRRSLQDLHHGLGIGNGMLSAFQP----PQHHQVQMPPPHP----- 1178
            :.|.|.:||...::...||.:.. :|.....|.|:     .:|    |||....:....|
Mouse  1074 KPNSAPQELTAASEETESNGQED-EDGEERPGTGD-----LEPDSAEPQHFTTTVTRCSPTVALV 1132

  Fly  1179 -----QQIYANVPQQQQQMVLHAYHQMDTDYRKSMSDLNE--FSNCLILPPTPPTKPLRAMQLNA 1236
                 .|:...||:|.|:..           ...||...|  .|..:..|.:|
Mouse  1133 EFSSNPQLKNEVPEQGQKKP-----------EDEMSGKVELVLSQKVAKPKSP------------ 1174

  Fly  1237 NGHGLEPDYAVS---------TRQRQPPAPYGGSLVKIAGAPMPPRLPGQGYPIQAPTAPAYHHQ 1292
                 ||:..::         |.|...|.|...     |.:|...:.||
Mouse  1175 -----EPEATLTFPFLDKMPETNQLHLPNPSSQ-----ADSPSSEKSPG---------------- 1213

  Fly  1293 SAQNLSNMSRNTLLALSATPKPKYADGWVQVQQRKSYDSQQTSDAAWLAAQQQKRKSMPDYGGAL 1357
                             :||...:|  |...::|:..:..|......|..:.||.:..
Mouse  1214 -----------------STPFKFWA--WDPEEERRRQEKWQQEQERLLQERYQKEQDK------- 1252

  Fly  1358 YNNNHWLLQEAEQRRIEQLNRRSIPASKSMGKPLPDSII----------QTLTERVQSKGIGDRK 1412
             ....|   |..|:.:|:..||.....:   |.:.|:::          |..|....::|.|.|.
Mouse  1253 -LKEEW---EKAQKEVEEEERRYYEEER---KIIEDTVVPFTISSSSADQLSTSLSVTEGSGTRN 1310

  Fly  1413 RFD------------SNGNYSQVNGNNIYQQQQ----QQKNVTNGSSIN---------------- 1445
            :.|            ....:.:.:|:::.:.::    :::::|...|.|
Mouse  1311 KMDLENCPDKENERRQKTPFQENDGDSLLKTREGGLPEEQSLTPSPSANPEISVSKGIHQDPQLE 1375

  Fly  1446 --------GNSSQ---------------------------------------------------- 1450
                    |.:.|
Mouse  1376 AEAGAPHCGTNPQPAQDPPRNQQIPNPPTSTSEDVKPKTLALEKTINHQMESPGERRKKNPRENF 1440

  Fly  1451 -------------GQEKVLSVSGKKKCSHCGDELGKLCYYVGCRGAAMIIESLLLFYHINCFKCC 1502
                         ||....|:||||.||.||..|||        |||||||:|.|::||.||:|.
Mouse  1441 RAGPLSPCPPTPPGQSPNRSISGKKLCSSCGLTLGK--------GAAMIIETLNLYFHIQCFRCG 1497

  Fly  1503 VCHVQLGDGLNGTDVRVRNHKLHCQNCY 1530
            :|..||||.::|||||:||..|:|.:||
Mouse  1498 ICKGQLGDAVSGTDVRIRNGLLNCTDCY 1525

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
smash	NP_001246912.1	DUF4757	<47..153	CDD:464950	24/120 (20%)
		PAT1	<1159..>1335	CDD:401645	32/200 (16%)
		LIM	<1494..1530	CDD:395333	19/35 (54%)
Limch1	NP_001388345.1	CH_LIMCH1	23..140	CDD:409127	20/92 (22%)
		DUF4757	250..>311	CDD:464950	12/67 (18%)
		DUF4757	<739..872	CDD:464950	37/224 (17%)
		GBP_C	<1225..1262	CDD:293879	7/47 (15%)
		LIM	1467..1524	CDD:214528	35/64 (55%)