DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment smash and Limch1

DIOPT Version :9

Sequence 1:	NP_001246912.1	Gene:	smash / 40583	FlyBaseID:	FBgn0263346	Length:	1541	Species:	Drosophila melanogaster
Sequence 2:	XP_036021493.1	Gene:	Limch1 / 77569	MGIID:	1924819	Length:	1511	Species:	Mus musculus

Alignment Length:	1723	Identity:	340/1723 - (19%)
Similarity:	574/1723 - (33%)	Gaps:	524/1723 - (30%)

- Green bases have known domain annotations that are detailed below.

  Fly    46 PYVTKQRVTRPTPPAPSKNPMQFVQIKPCNLYQTAQEQLKKAEEVKKLKEVKKEEPEEWQN---- 106
            |.:.|:....|||.|...|.:.|               |:..:|: .|||.:..:|.:.|:
Mouse    63 PGLVKKINRLPTPIAGLDNTILF---------------LRGCKEL-GLKESQLFDPSDLQDTSNR 111

  Fly   107 ----NLDNWKSSRRKRVE-HIIDRAVET------KKLELEEHDRLRRKSKTFTEMMEERAERGGP 160
                |||..:..:...|. :.:.:|..:      ..|.|:|.:.|      ..:|.:|..:...|
Mouse   112 VTVKNLDYSRKLKNVLVTIYWLGKAANSCASYGGTTLNLKEFEGL------LAQMRKETDDIDSP 170

  Fly   161 R--------------GRAKLASLAVYNEDETNDLSDLGIGTSSASGKSS---LSGDYDNNSVMSD 208
            :              .|:...|...:..|::.|..| ..|:.|....|.   |.|..|...  ||
Mouse   171 KRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLD-SFGSRSRQTPSPDVVLRGSSDGRG--SD 232

  Fly   209 HAAELDKAIGAASSVAAGVTAQNVDEQLNHIN-RNGSNGNHGNGAAVGQTGPSNSSKSAGRQYIS 272
            ..::|..             .:..|.:.:.:: |..|:|...:.....|..|:.|:::|   |:.
Mouse   233 SESDLPH-------------RKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTA---YVP 281

  Fly   273 SP-------------GYDTSSSNAQASSPDLCEYTYEGAIQDYKQRVQRAS-----SNGNGNANG 319
            :|             .:.|::|......|    ::|...|::  :.:...|     .:....|:|
Mouse   282 APLRKKKAEREEFRKSWSTATSPLGGERP----FSYPETIEE--EGIDAGSPTEDDPSSQVGADG 340

  Fly   320 KLIGEHIAYPTRRGSKIEDRLSGFEVTSPS---DTQEGVEKQKVDVPKVDISKRKEIFEQAKAEV 381
            |...|..|.|    |..||:..|.....|:   :.::.||.||         :||  .|||..:|
Mouse   341 KPDSELEAPP----SNGEDQSPGDAAVVPAALPEDRDAVESQK---------RRK--LEQAGIKV 390

  Fly   382 SNGGAPAAPKLVFRDGLTNGNSATTAPKSEVRRLSGDISSIRDRMQSLEQQRKAFSSSKSVDVPV 446
                .|||.:.           |:....||.:....||...::......|..|...|.......:
Mouse   391 ----MPAAQRF-----------ASQKQSSEEKEAIRDIVLRKENPFLTHQHGKDSESEDEAACLL 440

  Fly   447 PPLKQRLNSLQQSVAKEEQKKP--PLVALI-------DARQL----EIMRGEEE------RMRQQ 492
            |.|::  :......||..|.||  ||..|:       ||.:.    ::.:|..:      :..|.
Mouse   441 PDLEK--DDFAARRAKMNQSKPMVPLNQLLYGPYRNKDAEKAGGSKQLSKGLSDKKNLGFKRNQG 503

  Fly   493 QQKEKHSPSQTTLCAPKPAPPALIIEEPPVADTNDDSGIQEDTADELQQQQQLNAAIAALALEER 557
            |:::.::....|:...|..|......:.|  |.:.|...|......|...::..:.|....:.|.
Mouse   504 QREDVNTMVACTVQTQKDEPLETNFRKTP--DRHKDDLAQRRIQGRLTPHREAPSFIKLSGITEA 566

  Fly   558 QLEEAAN-AVNQIEAEFDELTDLHPSPLPPSPALPAVQQSLQQPTVAQVPAQAVNQAAAPPLRDM 621
            .||.... .|:....:.|......|.|.|......|::...     |...|:|..:|:.|..:.:
Mouse   567 DLETWERLKVSGETRDGDAQNTCAPEPSPEIDTETAIRDDF-----ASRKARAYKKASGPRQKFV 626

  Fly   622 EFSINEALELALQAIDREATTK--PMDKAQHPQKREQKEELGGKPE-------EKEPICEEQHDE 677
            .|  ....|:..|...|.:..|  |.|..:..:...|.:...|.|.       ::|.:...|.|.
Mouse   627 HF--GPVTEIDQQTWKRLSIGKAGPRDNVEVVRHSSQNQISSGSPPSTATFGLKEEQMLSPQSDC 689

  Fly   678 ESNEQGETHNEGGEMPNKTDKQMQGG--REPIYENVSSTISMHEVDNEQIATMTITTQTQATRIS 740
            .:            :|:.||.:::..  ..|:.|:          ..||:...:...:|:..
Mouse   690 RT------------VPSGTDGRLKKAPWLAPVLES----------QEEQVCIQSNNPKTEEV--- 729

  Fly   741 HRRSIPSQQTSNSNENNQSTNQTSNS---RNQKESSTAATSAANPVPPTTPGEQAEPYYQVPKAT 802
                .|:|...:..|.|:...|..:.   |.|:                 |.|.:.|.|
Mouse   730 ----TPTQLPQDGREQNEEVIQKEHEMLPRVQR-----------------PEECSGPLY------ 767

  Fly   803 EPYYDAPKHLRPVPVYENVE---IFYSGLEISQGSVGAPVGLMEPPKEKPPPPPTESPIPLDEGH 864
                 .|:    .||.::.|   :|....| .:.:|                      :|.....
Mouse   768 -----GPR----TPVSDDAESTSMFDMRCE-EEAAV----------------------LPHSRAR 800

  Fly   865 DDELDGLGNSLGHNTDTWSSDNTYETISNGTRRHLQGQPEPAQPSPPIKRMNSTKR------IKK 923
            .::|..:.|.|....|.|..|                          :.|..|.:|      |||
Mouse   801 QEQLQLINNQLREEDDKWQDD--------------------------LARWKSRRRSASQDLIKK 839

  Fly   924 ELRNKRSSFLGIENDGDLD---DMETYLELTVAPPPDMAQLLQEERRLEKQLYIKAGLCDSSDTG 985
            |...|:...|....||..:   .::||.|           ::||:.|.|::|:      ::....
Mouse   840 EEERKKMEKLMSGEDGTSERRKSIKTYRE-----------IVQEKERRERELH------EAYKNA 887

  Fly   986 ESRDSGVSENHSRQSSEHYT------------------NSSEENDTQSEATPPP--------LPP 1024
            .|::.  :|...:|..|.:|                  :|:|.|.:.....|.|        |||
Mouse   888 RSQEE--AEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNVSSFPNDPSPMKYLRQQSLPP 950

  Fly  1025 PPSTAQVGEVIYQN---ESLLAAQTPLLQTVKGNSAESWTESATAAAAATQSLSEATATANAKMQ 1086
            |..||.|...|.:.   ||:.:|.|       |:.::..|.:...............:....|..
Mouse   951 PKFTATVETTIARTSVPESIASAGT-------GSPSKIITPNTVPMLTPRPYSQPKNSQEVLKTF 1008

  Fly  1087 SIEDKIREQGEMLRVERE--------------------------LLHFSQEELKRQRENL---VL 1122
            .::.|:...|||...:.|                          .:|.|..::|:...::   :.
Mouse  1009 KVDGKVSMNGEMAPGDEEGKEKEGPAAAAPGPSLTKSQMFEGVATVHDSPVQVKQGSNSIEINIK 1073

  Fly  1123 RENIARRELQHGAKMLMSNNRRSLQDLHHGLGIGNGMLSAFQP----PQHHQVQMPPPHP----- 1178
            :.|.|.:||...::...||.:.. :|.....|.|:     .:|    |||....:....|
Mouse  1074 KPNSAPQELTAASEETESNGQED-EDGEERPGTGD-----LEPDSAEPQHFTTTVTRCSPTVALV 1132

  Fly  1179 -----QQIYANVPQQQQQMVLHAYHQMDTDYRKSMSDLNE--FSNCLILPPTPPTKPLRAMQLNA 1236
                 .|:...||:|.|:..           ...||...|  .|..:..|.:|
Mouse  1133 EFSSNPQLKNEVPEQGQKKP-----------EDEMSGKVELVLSQKVAKPKSP------------ 1174

  Fly  1237 NGHGLEPDYAVS---------TRQRQPPAPYGGSLVKIAGAPMPPRLPGQGYPIQAPTA-----P 1287
                 ||:..::         |.|...|.|...     |.:|...:.||     ..|..     |
Mouse  1175 -----EPEATLTFPFLDKMPETNQLHLPNPSSQ-----ADSPSSEKSPG-----STPFKFWAWDP 1224

  Fly  1288 AYHHQSAQNLSNMSRNTLLALSATPKPKYADGW------VQVQQRKSYDSQQT---------SDA 1337
            ....:..:.........|.......:.|..:.|      |:.::|:.|:.::.         :.:
Mouse  1225 EEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTIS 1289

  Fly  1338 AWLAAQQQKRKSMPDYGGALYNNNHWLLQEAEQRRIEQLNRRSIPASKSMGKPLPDSII------ 1396
            :..|.|.....|:.:..|   ..|...|:....:..|:  |:..|..::.|    ||::
Mouse  1290 SSSADQLSTSLSVTEGSG---TRNKMDLENCPDKENER--RQKTPFQENDG----DSLLKTREGG 1345

  Fly  1397 ----QTLT-------ERVQSKGIGDRKRFDSNGNYSQVNGNNIYQQQ-----QQQKNVTNGSS-- 1443
                |:||       |...||||....:.::....... |.|....|     ||..|....:|
Mouse  1346 LPEEQSLTPSPSANPEISVSKGIHQDPQLEAEAGAPHC-GTNPQPAQDPPRNQQIPNPPTSTSED 1409

  Fly  1444 -----------INGNSSQGQEKVLSVSGKKKCSHCGDELGKLCYYVGCRGAAMIIESLLLFYHIN 1497
                       ||.......|:..|:||||.||.||..|||        |||||||:|.|::||.
Mouse  1410 VKPKTLALEKTINHQMESPGERRKSISGKKLCSSCGLTLGK--------GAAMIIETLNLYFHIQ 1466

  Fly  1498 CFKCCVCHVQLGDGLNGTDVRVRNHKLHCQNCY 1530
            ||:|.:|..||||.::|||||:||..|:|.:||
Mouse  1467 CFRCGICKGQLGDAVSGTDVRIRNGLLNCTDCY 1499

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
smash	NP_001246912.1	DUF4757	14..152	CDD:292571	25/120 (21%)
smash	NP_001246912.1	LIM	<1494..1530	CDD:278823	19/35 (54%)
Limch1	XP_036021493.1	CH_LIMCH1	23..140	CDD:409127	20/92 (22%)
		DUF4757	250..>311	CDD:406382	12/67 (18%)
		DUF4757	<739..872	CDD:406382	37/224 (17%)
		GBP_C	<1225..1262	CDD:419202	3/36 (8%)
		LIM	1441..1498	CDD:214528	35/64 (55%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG1704
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0007310
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	0	0.000	Not matched by this tool.
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R4167
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	3	2.930