DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment smash and limch1a

DIOPT Version :9

Sequence 1:	NP_001246912.1	Gene:	smash / 40583	FlyBaseID:	FBgn0263346	Length:	1541	Species:	Drosophila melanogaster
Sequence 2:	XP_021330942.1	Gene:	limch1a / 561940	ZFINID:	ZDB-GENE-090312-153	Length:	1960	Species:	Danio rerio

Alignment Length:	2101	Identity:	395/2101 - (18%)
Similarity:	629/2101 - (29%)	Gaps:	820/2101 - (39%)

- Green bases have known domain annotations that are detailed below.

  Fly     6 QEVMLEGRE-----DLMSTSATSSTNGGDTNGCGKKLSVTSPDPTPYVTKQRVTRPTPPAPSKNP 65
            :||.|:|.:     ||..||..::..|.|   |.:||                         ||.
Zfish    92 EEVGLKGSQLFDPGDLQDTSIRANLTGSD---CSRKL-------------------------KNV 128

  Fly    66 MQFV-----QIKPCNLYQTAQEQLKKAEEVKKL--KEVKKEEPEEWQNN------LDNWKSSR-- 115
            :..:     ....|..|......||:.|.:..:  ||...:|.:..:.:      :|:|:|.|
Zfish   129 LITIYWLGKAANSCATYNGPTLDLKEFEGLLSMMRKECVNDESDSPKRSIRDSGYIDSWESERSD 193

  Fly   116 ---------------------RKRVEHIIDRAVETKKLELEEHDRLRRKSKTFTEMMEERAERGG 159
                                 |.:.....|..:.....:.|...:|....|  .:|...|.....
Zfish   194 SLSPPRHGRDDSFDSLDSFGSRSQNTPSPDTVIRCNSDDSEADRKLPDVRK--DDMSARRVSYKE 256

  Fly   160 PRGRAKL---------------ASLAVYNEDETNDLSDLGIGTSSASGKSSLSGD----YDNNSV 205
            ||.....               |.|.....|...|...:...||...|:..|..:    ....:|
Zfish   257 PRAALPFNQYLPNKSNQTNYLPAPLRKRRSDREEDRRSVSNSTSPVGGERPLRQECVEVLKEGAV 321

  Fly   206 MS------------DHAAELDKAIGAASSVAAGVTAQNVDEQLNHINRNGSNGNHGNGAAVGQTG 258
            :|            :..||||:|                  :|..:.:....|.....|||....
Zfish   322 VSQKKVQKHFTWMVEKGAELDEA------------------ELKKMRKLEKAGIKVLPAAVRYNS 368

  Fly   259 PSNSSKSAGRQYISSPGYDTSSSNAQASSPDLCEYTYEGAIQDYKQRVQRASSNGNGNANGKLIG 323
            |           |.||   ..:|..::.|||:        |........||
Zfish   369 P-----------IVSP---AEASKPKSPSPDI--------ILRRDNDFMRA-------------- 397

  Fly   324 EHIAYPTRRGSKIEDRLSGFEVTSPSDTQEGVEKQKVDVPKVDISKRKEIFEQAKAEVSNGGAPA 388
               |.|.:..|..|:.            .|..|::..||.|.|::.|:....|.|..|::...|:
Zfish   398 ---AVPHQWDSDEEEE------------DEEAEQKVPDVQKDDLASRRARMNQFKPSVAHHFLPS 447

  Fly   389 APKLVFRDGLTNGNSATTAPKSEVRRLSGDISSIRDRMQSLEQ---QRKAFSSSKSVDVPVPPLK 450
            :.....|:            |.|..|::.. .::.:||:.:||   ::::.:.|.|.:||:...|
Zfish   448 SCSWKDRE------------KWEGIRMASQ-HAVLERMEKMEQEKLEKRSQTESASPEVPIITRK 499

  Fly   451 QR-LNSLQQSVAKEEQKKPPLVALIDARQLEIMRGEEERMRQQQQKEK-HSPSQTTLCAPKPAPP 513
            .. ..||::..|||                    |:||..|:::.|.. .:|.:..|...:....
Zfish   500 DNPFLSLEKDRAKE--------------------GDEEDEREEEGKTVIPNPHKDDLARRRTGGR 544

  Fly   514 ALIIEEPPVADTNDDSGIQED-TADELQQQQQLNAAIAALALEERQLEEAANAVNQIEAEFDELT 577
            ..|.::|      ..|.:|.. |..:|:..|:|.     :..|..:.:.|...|:.|..:
Zfish   545 PRIPKDP------YQSLVQTSITQSDLETWQRLK-----MNTESSESDSAPAEVSIITRK----- 593

  Fly   578 DLHPSPLPPSPALPAVQQSLQQ---------------PTV-AQVPAQAVNQAAAPPLRDMEFSIN 626
                    .:|.|...:||.|:               |.| |...|:..:|:.:.||::...|:.
Zfish   594 --------ENPFLTPEKQSEQETVEGASREEGERVTTPNVKADDLARRRSQSGSLPLKEPRQSLA 650

  Fly   627 EALELALQAIDREATTKPMDKAQHPQKREQKEELGGKPEE-------KEP-ICEEQHDEESNEQG 683
            :.          ..|...::|.|..:......|....|.|       :.| :..|:..|:.||:.
Zfish   651 QT----------TITQSDLEKWQKLKMSTDSSESDSAPAEVSIITRKENPFLTPEKQSEQENEES 705

  Fly   684 ETHNEGGEM--PN-KTD----KQMQGG----REPIYENVSSTISMHEVDNEQIATMTITTQTQ-- 735
            ....||..:  || |||    ::.|.|    :||......:||:..:::..|...|:..:..:
Zfish   706 ANREEGERVMTPNVKTDDLARRRSQSGSLLQKEPRQSLAQTTITQTDLEKWQKLKMSTDSSDEPI 770

  Fly   736 ---------------ATRISHRRSIPSQQTSNSNENNQS-------TNQTSNSRNQKESSTAATS 778
                           |::....::.|:....:|:...:.       |...|.|..::|.......
Zfish   771 VPLCQTCLEKGCLSVASKTKFAKNDPAGCWDHSSGERRRFVTFGGVTEMESTSWKEEEEEEEEED 835

  Fly   779 AANPVPPTTPGEQAEPYYQVPKATEPYYDAPKHLRPVPVYENVEIFYSGL--EISQGSVGAPVGL 841
            ..:.......|.:.|                 .||.:.....|.:...||  .:::.:||:....
Zfish   836 GEDEEKGEREGNEGE-----------------KLRYLLSVAPVAVPTIGLSSRLTKRAVGSGDVN 883

  Fly   842 MEPPKEKPPPPPTESPI------PL-----------------DEGHDDELD-------------- 869
            .||....||.||  :|.      ||                 ||..|||.|
Zfish   884 PEPAPIMPPEPP--NPFMFDLKRPLTSTERRVLEDELVEKAKDEKDDDEEDEAEERQPDIEKDDM 946

  Fly   870 ------------------------------GLGNSL--------GHNTDTWSSDNTYETISNGTR 896
                                          .:|.||        |.:.|   .|..|...:..|:
Zfish   947 LARRTRAFKKTAGGTSFNQFLPLPGSKREGTVGGSLAQKGGVLIGSSGD---RDLHYLERNIRTK 1008

  Fly   897 RHLQGQP-EP------------AQPSPPIKRMNSTKRIKKELRNKRSSFLGIENDGDLDDMETYL 948
            |...||. ||            .|.||.|.|..::       ...||:    ..|.|.||.:.|:
Zfish  1009 RTKIGQRFEPVDQVAKTPVAVVTQQSPDIVRSTAS-------NTDRSA----SCDYDDDDDDEYV 1062

  Fly   949 ELTVAPPPDMAQLLQEERRLEKQLYIKAGLCDSSDTGESRDSGVSENHSRQSS--------EHYT 1005
            :...:|..:...:|  .||........||...|.:....:...|....:..|.        ||.|
Zfish  1063 DDGQSPYVEKDDML--ARRTGSYQKPNAGAGQSFNAFLPKPGAVKHKITPVSGSQSCVKPREHET 1125

  Fly  1006 NSSEENDTQSEATPPP---------LPPPPSTAQVG----EVIYQNESLLAAQTPLLQTVKGNSA 1057
            ..|:::.|.|...|||         .|......:.|    |.:.::|::..:.:|:.:  :.:..
Zfish  1126 APSQDSFTLSSVAPPPRDAEDMPRKWPQMMDRQEAGSGGQESLIKHETITKSLSPMPE--QPSRR 1188

  Fly  1058 ESWTE---SATAAAAATQSLSEATATANAKMQSIED---------------------KIRE---- 1094
            ..|.|   .......||....||.:.:...|:..||                     |::|
Zfish  1189 PLWQEDDDDLPPIFRATSVSDEAESVSLIDMRDEEDELAYLRPHSQSRHEHLHNQYNKVKEEEDQ 1253

  Fly  1095 -QGEMLRVERELLHFSQEELKRQRENLVLRENIARRELQHG--------AKMLMSNNRRSLQDLH 1150
             |.::.|.:......||:.:|::.|..:: |.:...:..||        .|.::....:..|:||
Zfish  1254 WQDDLARWKNRRRSASQDLIKKEEERKMM-EKLMTTDGGHGHRKKSIKTYKEIVEEKEKREQELH 1317

  Fly  1151 H--------------------GLGIGNGMLSAFQPPQHHQVQMP----PPH-PQQ-------IYA 1183
            .                    ...|.:.:|...|.|:......|    |.| |:|       :.|
Zfish  1318 EQYCKASSPEEKAAVLQRYALRFTISDAILEKLQLPKLPSAASPMHTSPLHQPEQKTTSTTLVEA 1382

  Fly  1184 NVPQ----QQQQMVLHAYHQMDTDYRKSMSDLNEFSNCLILPPTPPTKPLRAMQLNANGHGLEPD 1244
            .||:    .|.|..:|...::..|...:...:.:        ||||.|.::|.::
Zfish  1383 EVPELSQTTQTQQTVHVKQKITHDEAPAQPKIPK--------PTPPPKCIKAPEV---------- 1429

  Fly  1245 YAVSTRQRQPPAPYGGSLVKIAGAPMPPRLP--------GQGY---------------------- 1279
              :|...|.||||         ..|:|...|        .||:
Zfish  1430 --LSAPSRVPPAP---------SKPVPLITPKPYSQPKFSQGFRKSLKSDGLIRVNGDHNQQVIR 1483

  Fly  1280 ------PIQAP------TAPAYHHQSAQN-----LSNMSRNTLLALSATPKP--------KYADG 1319
                  |:|:|      :.|....||.||     |........|   .||||        |..:.
Zfish  1484 EKANRDPLQSPEVNFSSSPPKPEIQSQQNEDPIVLETTDHQQDL---QTPKPDLEKEVIKKEEES 1545

  Fly  1320 WVQVQQRKSYDSQQT-------------------------------------------------- 1334
            .:..|:..|.:...|
Zfish  1546 LLSPQKEISKEDTSTTLSVSEPAGKQPVPDTVMKRNFVVTTTIVTELTQTHPVPLEDTSSTEQPD 1610

  Fly  1335 --------SDAAWLAAQQQKRKSMPDYGGALYN--------------NNHW-------------- 1363
                    |..|......|...|:....|..|:              .:||
Zfish  1611 TVQNTCDLSSTAESCKADQSSYSISSTEGIDYSIECIETPMLNLAKRVDHWTWDPNEERRRQERW 1675

  Fly  1364 ------LLQEAEQRRIEQLNR---------------------------------RSIPASKSMGK 1389
                  ||||..||..|:|..                                 |:...|.|.|.
Zfish  1676 QQEQERLLQEKYQREQEKLKEEWEKAQREVEEEERRHHEEERRILEETVTPLTPRTSALSSSCGT 1740

  Fly  1390 PLP---------DSIIQTLT--ERVQSKGIGDRKRFDSNG---------NYSQVNGNNIYQQQQQ 1434
            ..|         ::|:.:|.  ||.|.......:...|||         ..:|.||.....|:.|
Zfish  1741 EAPSLTQPSAPHETIVLSLADWERKQEMLEKQAQTGQSNGLTHSPDPVIAAAQQNGQRAEAQESQ 1805

  Fly  1435 ----------------------QKNVTNGSSINGNSSQGQ------------EKVL--------- 1456
                                  |:::...:|::...|...            |.||
Zfish  1806 ITATPQLQFIQDGSWSCKSKGRQEDLKKTASLDRKQSPAPSQTTGMRRTGSCENVLGTHPSKSPT 1870

  Fly  1457 ------------SVSGKKKCSHCGDELGKLCYYVGCRGAAMIIESLLLFYHINCFKCCVCHVQLG 1509
                        ||||||.||.|...|||        |||||||:|.|::||.||||.||...||
Zfish  1871 PSQDTPPPSPSRSVSGKKLCSSCAHPLGK--------GAAMIIETLGLYFHIQCFKCGVCKGLLG 1927

  Fly  1510 DGLNGTDVRVRNHKLHCQNCY 1530
            |...|||||:||..|:|..||
Zfish  1928 DTSAGTDVRIRNGLLNCHECY 1948

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
smash	NP_001246912.1	DUF4757	14..152	CDD:292571	29/178 (16%)
smash	NP_001246912.1	LIM	<1494..1530	CDD:278823	20/35 (57%)
limch1a	XP_021330942.1	CH	27..>107	CDD:237981	4/14 (29%)
		DUF4757	247..>310	CDD:318215	12/62 (19%)
		DUF4757	<1166..1308	CDD:318215	24/144 (17%)
		LIM	1890..1952	CDD:278823	37/67 (55%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0007310
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	0	0.000	Not matched by this tool.
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R4167
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	2	2.030