DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment smash and Limch1

DIOPT Version :9

Sequence 1:	NP_001246912.1	Gene:	smash / 40583	FlyBaseID:	FBgn0263346	Length:	1541	Species:	Drosophila melanogaster
Sequence 2:	XP_038947830.1	Gene:	Limch1 / 305332	RGDID:	1305269	Length:	1561	Species:	Rattus norvegicus

Alignment Length:	1837	Identity:	337/1837 - (18%)
Similarity:	581/1837 - (31%)	Gaps:	702/1837 - (38%)

- Green bases have known domain annotations that are detailed below.

  Fly    46 PYVTKQRVTRPTPPAPSKNPMQFVQIKPCNLYQTAQEQLKKAEEVKKLKEVKKEEPEEWQN---- 106
            |.:.|:....|||.|...|.:.|               |:..:|: .|||.:..:|.:.|:
  Rat    63 PGLVKKINRLPTPIAGLDNTILF---------------LRGCKEL-GLKESQLFDPSDLQDTSNR 111

  Fly   107 -NLDNWKSSRRKR----VEHIIDRAVET------KKLELEEHDRLRRKSKTFTEMME-------- 152
             .:.|:..||:.:    ..:.:.:|..:      ..|.|:|.:.|..:.:..|:.:|
  Rat   112 VTVKNFDYSRKLKNVLVTIYWLGKAANSCTSYSGTTLNLKEFEGLLAQMRKETDDIESPKRSIRD 176

  Fly   153 ---------ERAERGGP--RGRAKLASLAVYNEDETNDLSDLGIGTSSASGKSS---LSGDYDNN 203
                     ||::...|  .||           |::.|..| ..|:.|....|.   |.|..|..
  Rat   177 SGYIDCWDSERSDSLSPPRHGR-----------DDSFDSLD-SFGSRSRQTPSPDVVLRGSSDGR 229

  Fly   204 SVMSDHAAELDKAIGAASSVAAGVTAQNVDEQLNHIN-RNGSNGNHGNGAAVGQTGPSNSSKSAG 267
            .  ||..::|..             .:..|.:.:.:: |..|:|...:.....|..|:.|:::|
  Rat   230 G--SDSESDLPH-------------RKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTA- 278

  Fly   268 RQYISSPGYDTSSSNAQASSPDLCEYTYEGAIQDYKQRVQRASSNGNGNANGKLIGEH-IAYPTR 331
              |:.:|   .....|:.              ::|::....|:|        .|.||. .:||
  Rat   279 --YVPAP---LRKKKAER--------------EEYRKSWSTATS--------PLGGERPFSYP-- 314

  Fly   332 RGSKIEDRLSGFEVTSPSDTQEGVEKQKVDVPKVDISKRKEIFEQAKAEVSNGGAPAAPKLVFRD 396
              ..||:  .|.|..||::.....:......|:..:          :|..|||.|.:.
  Rat   315 --ETIEE--EGSEAGSPTEDDPSSQMDPDGKPECGL----------EAPPSNGKAQSP------- 358

  Fly   397 GLTNGNSATTAPKSEVRRLSGDISSIRDRMQSLEQQRKAFSSSKSVDVPVPPLKQRLNSLQQSVA 461
                 ..|...|           ::|.:..:::|:|::  ...:...:.|.|..||..|.:||..
  Rat   359 -----EDAAAVP-----------AAIPEDREAVERQKR--RRLEQAGIKVMPAAQRFASQKQSSE 405

  Fly   462 KEEQKKPPLVALIDARQLEIMRGEEERMRQQQQKEKHSPSQTTLCAP-----------------K 509
            ::|       |:.|.    |:|.|...:..|..|:..|..:.....|                 |
  Rat   406 EKE-------AIRDI----ILRKENPFLAHQHGKDSESEDEAACLLPDLEKDDFAARRARMNQRK 459

  Fly   510 PAPPALIIEEPPVADTNDDS------------------GIQEDTADELQQQQQLNAAIAALALEE 556
            |..|...:...|.. .||::                  |.:.|..    .::.:|..:|.:
  Rat   460 PMVPLNQLLYGPYR-KNDEAKAGSSQHLSKGPSDKKNLGYKRDQG----HREDVNRVVACV---- 515

  Fly   557 RQLEEAANAVNQIEAEFDELTDLHPSPLPPSPALPAVQQSLQQPTVAQVPAQAVNQAAAPPLRDM 621
            .|.:|:    ..:|..|.:..||....|........:.|..:.|:..::..          :.:.
  Rat   516 MQTQES----EPLEMSFRKAPDLQKDDLAQRRIQGRLSQHREAPSFIKLSG----------ITEA 566

  Fly   622 EFSINEALELALQAIDREATTKPMDKAQHPQKREQKEELGGKPEEKEPICEEQHDEESNEQGETH 686
            :....|.|:::.:..|.:|                           :..|..:...|.:.:....
  Rat   567 DLETWERLKVSGETRDGDA---------------------------QNTCAPEPSPEIDAETAIR 604

  Fly   687 NEGGEMPNKTDKQMQGGRE------PIYENVSST---ISMHEV---DNEQIATMTITTQTQATRI 739
            ::......:..|:..|.|:      |:.|....|   :|:.:.   ||.::.:.:..::|.:
  Rat   605 DDFASRKARAYKKAPGPRQKFVHFGPVTEIDQQTWQRLSIGKAGPRDNVEVLSHSSESRTSS--- 666

  Fly   740 SHRRSIPSQQTSNSNENNQSTNQT--------SNSRNQK----------ESSTAATSAANP---- 782
               ||.||..|.:..|....:.|:        ::.|.:|          :.......:.||
  Rat   667 ---RSPPSTTTFDLKEEQMLSPQSDYRTVPSGTDGRLKKAPWLAPVLESQEEQVCVQSNNPKTEE 728

  Fly   783 VPPT-TPGEQAEPYYQVPKATEPYYDAPKHLRPVPVYENVEIFYSGLEISQGSVGAPVGLMEPPK 846
            |.|| .|.|:.|...:.|:.      .|:..||                 :||.|...|...|..
  Rat   729 VTPTCLPQEKEEVIKKEPEM------LPRVQRP-----------------EGSSGPLYGPRTPVS 770

  Fly   847 EKPPPPPTESP-------------IPLDEGHDDELDGLGNSLGHNTDTWSSDNTYETISNGTRRH 898
            :     ..||.             :|......::|..:.|.|....|.|..|
  Rat   771 D-----DAESTSMFDMRCEEEAAVLPHSRARQEQLQLINNQLREEDDKWQDD------------- 817

  Fly   899 LQGQPEPAQPSPPIKRMNSTKR------IKKELRNKRSSFLGIENDGDLD---DMETYLELTVAP 954
                         :.|..|.:|      ||||...|:...|....||..:   .::||.|
  Rat   818 -------------LARWKSRRRSASQDLIKKEEERKKMEKLMSGEDGTSERRKSIKTYRE----- 864

  Fly   955 PPDMAQLLQEERRLEKQLYIKAGLCDSSDTGESRDSGVSENHSRQSSEHYT-------------- 1005
                  ::||:.|.|::|:      ::.....|::.  :|...:|..|.:|
  Rat   865 ------IVQEKERRERELH------EAYKNARSQEE--AEGILQQYIERFTISEAVLERLEMPKI 915

  Fly  1006 ----NSSEENDTQSEATPPP--------LPPPPSTAQVGEVIYQNESLLAAQTPLLQTVKGNSAE 1058
                :|:|.|.:.....|.|        ||||..||.|             :|.:.:|
  Rat   916 LERSHSTEPNVSSFPNDPSPMKYLRQQSLPPPKFTATV-------------ETTIART------- 960

  Fly  1059 SWTESATAAAAATQSLSEATATANA-------------------KMQSIEDKIREQGEMLRVERE 1104
            |..||:.||...:.|   .|:|.|.                   |...::.|:...||..|.:.|
  Rat   961 SVPESSVAAGTGSPS---KTSTPNTVPMLTPRPYSQPKNSQEVLKTFKVDGKVSMNGETARGDVE 1022

  Fly  1105 --------------------------LLHFSQEELKRQRENL---VLRENIARRELQHGAKMLMS 1140
                                      .:|.|..::|:...::   :.:.|...:||...::...|
  Rat  1023 GKEKEDPTAVAPGPSLTKSQMFEGVATVHGSPVQVKQGSNSIEINIKKPNSPPQELTAASEETES 1087

  Fly  1141 NNRRSLQDLHHGLGIGNGMLSAFQPPQHHQVQMPPPHP----------QQIYANVPQQQQQMVLH 1195
            |.|.. ::.....|..:..|.:.: |||....:....|          .|:...||::|.|
  Rat  1088 NGRDD-ENGEESSGARDVELDSAE-PQHFTTTVTRCSPTVALVEFSSSPQLRNEVPEEQDQ---- 1146

  Fly  1196 AYHQMDTDYRKSMSDLNEFSNCLILPPTPPTKPLRAMQLNANGHGLEPDYAVSTRQRQPPAPYGG 1260
                        ....||.|.                         :.:..:|.:..:|.:|...
  Rat  1147 ------------KKPENEMSG-------------------------KVELVLSQKVAKPKSPEPE 1174

  Fly  1261 SLVKIAGAP----MP-------PRLPGQGYPIQAP-------TAPAYHHQSAQNLSNMSRNTLLA 1307
            :.:..   |    ||       |.|..|..|..||       ....:..|||.:.|:..     :
  Rat  1175 ATLTF---PFLDKMPETDQLHLPNLNSQELPGSAPLRVQNSWRRSQFFSQSADSPSSEK-----S 1231

  Fly  1308 LSATPKPKYADGWVQVQQRKSYDSQQTSDAAWLAAQQQKRKSMPDYGGALYNNNHWLLQEAEQRR 1372
            .::||...:|  |...::|:..:..|......|..:.||.:..        ....|   |..|:.
  Rat  1232 PASTPFKFWA--WDPEEERRRQEKWQQEQERLLQERYQKEQDK--------LKEEW---EKAQKE 1283

  Fly  1373 IEQLNRRSIPASKSMGKPLPDSII----------QTLTERVQSKGIGDRKRFD------------ 1415
            :|:..||.....:   |.:.|:::          |..|....::|.|.|.:.|
  Rat  1284 VEEEERRYYEEER---KIIEDTVVPFTISSSSADQLSTSSSVTEGSGTRNKMDLENCQDRDKERR 1345

  Fly  1416 ------SNGNYSQV--------------------NGNN-----IYQQQQQQKNV------TNGSS 1443
                  .|.:.|.:                    |..|     |.|.||.:...      ||..|
  Rat  1346 QNTPLQENDSDSSLKARESGLPEEHSSLTQSPSANSENSVSKGISQDQQPETEAEASHCGTNPQS 1410

  Fly  1444 I----------NGNSSQGQE---KVL--------------------------------------- 1456
            .          |..:|:.::   |.|
  Rat  1411 AQDPPWNQQISNPPTSKSEDVKPKTLALEKSINHQIESPGERRKKSPRENFRAGPLSPCSPTPPG 1475

  Fly  1457 --------SVSGKKKCSHCGDELGKLCYYVGCRGAAMIIESLLLFYHINCFKCCVCHVQLGDGLN 1513
                    |:||||.||.||..|||        |||||||:|.|::||.||:|.:|..||||.::
  Rat  1476 QSPNRYKRSISGKKLCSSCGLALGK--------GAAMIIETLNLYFHIQCFRCGICKGQLGDAVS 1532

  Fly  1514 GTDVRVRNHKLHCQNCY 1530
            |||||:||..|:|.:||
  Rat  1533 GTDVRIRNGLLNCTDCY 1549

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
smash	NP_001246912.1	DUF4757	14..152	CDD:292571	24/120 (20%)
smash	NP_001246912.1	LIM	<1494..1530	CDD:278823	19/35 (54%)
Limch1	XP_038947830.1	CH_LIMCH1	23..140	CDD:409127	19/92 (21%)
		DUF4757	250..>311	CDD:406382	16/88 (18%)
		PRK07764	<276..>412	CDD:236090	38/211 (18%)
		DUF4757	<737..868	CDD:406382	34/195 (17%)
		GBP_C	<1245..1282	CDD:419202	7/47 (15%)
		LIM	1491..1548	CDD:214528	35/64 (55%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG1704
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0007310
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	2	1.900