Protein Alignment 5-HT2A and Htr1f

DIOPT Version :10

• Sequence 1: NP_730859.1
• Gene: 5-HT2A / 40575
• FlyBaseID: FBgn0087012
• Length: 930
• Species: Drosophila melanogaster
• Sequence 2: NP_068629.2
• Gene: Htr1f / 60448
• RGDID: 71083
• Length: 366
• Species: Rattus norvegicus
• Alignment Length: 574
• Identity: 115/574 - (20%)
• Similarity: 185/574 - (32%)
• Gaps: 262/574 - (45%)

  Fly   303 NILVCLAVALDRKLQNVTNYFLFSLAIADLLVSLFVMPMGAIPAFLGYWPLGFTWCNIYVTCDVL 367
            |.||..|:.:.|||.:..||.:.|||:.|.||::.|||...:......|.:|...|:::::.|::
  Rat    41 NSLVITAIIVTRKLHHPANYLICSLAVTDFLVAVLVMPFSIVYIVSESWIMGQGLCDLWLSVDII 105

  Fly   368 ACSSSILHMCFISLGRYMGIRNPLGSRHRSTKRLTGIKIAIVWVMAMMVSSSITVLGLVNEKNIM 432
            .||.||||:..|:|.||..|.:.:....:.|.|..||.|..|||:::.:|              |
  Rat   106 CCSCSILHLSAIALDRYRAITDAVEYARKRTPRHAGITITTVWVISVFIS--------------M 156

  Fly   433 P----------EPNICVINNRAFF--VFGSLVAFYIPMLMMVTTYALTIPLLRKKARFAAEHPES 485
            |          ..:.|:|.:....  ::.:..|||||:::::..|                   .
  Rat   157 PPLFWRHQGNSRDDQCIIKHDHIVSTIYSTFGAFYIPLVLILILY-------------------Y 202

  Fly   486 ELFRRLGGRFTLRPQHSQQQLQMFSSFSGSNNKFLSMGDGNRNFNTEGEMEEGGAPSRRSGVEPA 550
            :::|                                                            |
  Rat   203 KIYR------------------------------------------------------------A 207

  Fly   551 ERPLMQQRTASSRSMGTVSFRNVVNGTSGAAGSGRRTTGTAHSSFRFSGAGIFRHSSSSPASSCH 615
            .|.|..:|.||.      ..:..:|| .....||.::.....:|:      :...|.|.|
  Rat   208 ARTLYHKRQASR------MIKEELNG-QVLLESGEKSIKLVSTSY------MLEKSLSDP----- 254

  Fly   616 STSTSRSSSFWRKHGGYPNLMDRLAIHLRLSLVPPSSHPNRRASVRVTISQPQLGYPTNVGGNGG 680
                  |:.|.|.|.                                |:..|:
  Rat   255 ------STDFDRIHS--------------------------------TVKSPR------------ 269

  Fly   681 RTVGATEVGAGGNGSSAGRTSNSSLGGTSLMKIATIQGPILNQSQAAGGNHMVQKVKPTALKPEE 745
                                                                      :.||.|:
  Rat   270 ----------------------------------------------------------SELKHEK 276

  Fly   746 PQRNKVRPFKFAFNRVATPTLNLRFLNNRSKRNSLSANAVATEQKATKVLGLVFFTFVLCWSPFF 810
            ..|                            |..:|.   ..|:||...|||:...||:||.|||
  Rat   277 SWR----------------------------RQKISG---TRERKAATTLGLILGAFVICWLPFF 310

  Fly   811 ILNIIFAACPECQVPEHVVNTCLWLGYVSSTINPIIYTIFNRTFRAAFIRLLKC 864
            :..::...|.:|::.|.:.|...||||::|.|||:||||||..|:.||.:|::|
  Rat   311 VKELVVNICEKCKISEEMSNFLAWLGYLNSLINPLIYTIFNEDFKKAFQKLVRC 364

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
5-HT2A	NP_730859.1	7tm_GPCRs	298..>473	CDD:475119	52/181 (29%)
		TM helix 2	321..343	CDD:410628	11/21 (52%)
		TM helix 3	359..381	CDD:410628	8/21 (38%)
		TM helix 4	404..420	CDD:410628	6/15 (40%)
		TM helix 5	443..466	CDD:410628	5/24 (21%)
		7tm_GPCRs	<779..858	CDD:475119	33/78 (42%)
		TM helix 6	792..814	CDD:410628	10/21 (48%)
		TM helix 7	826..851	CDD:410628	14/24 (58%)
Htr1f	NP_068629.2	7tmA_5-HT1F	24..359	CDD:320456	112/567 (20%)
		TM helix 1	25..51	CDD:320456	4/9 (44%)
		TM helix 2	58..84	CDD:320456	11/25 (44%)
		TM helix 3	96..126	CDD:320456	12/29 (41%)
		DRY motif, important for ligand-induced conformation changes. /evidence=ECO:0000250|UniProtKB:P41595	120..122		0/1 (0%)
		TM helix 4	138..160	CDD:320456	10/35 (29%)
		TM helix 5	178..207	CDD:320456	7/107 (7%)
		TM helix 6	286..316	CDD:320456	13/29 (45%)
		TM helix 7	326..351	CDD:320456	14/24 (58%)
		NPxxY motif, important for ligand-induced conformation changes and signaling. /evidence=ECO:0000250|UniProtKB:P41595	343..347		2/3 (67%)