DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment 5-HT2A and HTR2A

DIOPT Version :9

Sequence 1:	NP_730859.1	Gene:	5-HT2A / 40575	FlyBaseID:	FBgn0087012	Length:	930	Species:	Drosophila melanogaster
Sequence 2:	NP_000612.1	Gene:	HTR2A / 3356	HGNCID:	5293	Length:	471	Species:	Homo sapiens

Alignment Length:	727	Identity:	170/727 - (23%)
Similarity:	260/727 - (35%)	Gaps:	316/727 - (43%)

- Green bases have known domain annotations that are detailed below.

  Fly   236 LGIMNVTNTSLDMFLPNLTALN----------TTSTASTAD-------------MATEFAVTK-- 275
            :.|:...||||.....:|..||          .:..|:|:|             ::.|..::.
Human     1 MDILCEENTSLSSTTNSLMQLNDDTRLYSNDFNSGEANTSDAFNWTVDSENRTNLSCEGCLSPSC 65

  Fly   276 -SFLDYSPHGYDFLFLFVVFFIFAGGLGNILVCLAVALDRKLQNVTNYFLFSLAIADLLVSLFVM 339
             |.|......:..|...||..:...  |||||.:||:|::||||.|||||.||||||:|:...||
Human    66 LSLLHLQEKNWSALLTAVVIILTIA--GNILVIMAVSLEKKLQNATNYFLMSLAIADMLLGFLVM 128

  Fly   340 PMGAIPAFLGY-WPLGFTWCNIYVTCDVLACSSSILHMCFISLGRYMGIRNPLGSRHRSTKRLTG 403
            |:..:....|| |||....|.:::..|||..::||:|:|.|||.||:.|:||:.....:::....
Human   129 PVSMLTILYGYRWPLPSKLCAVWIYLDVLFSTASIMHLCAISLDRYVAIQNPIHHSRFNSRTKAF 193

  Fly   404 IKIAIVWVMAMMVSSSITVLGLVNEKNIMPEPNICVINNRAFFVFGSLVAFYIPMLMMVTTYALT 468
            :||..||.:::.:|..|.|.||.::..:..|.: |::.:..|.:.||.|:|:||:.:||.||.||
Human   194 LKIIAVWTISVGISMPIPVFGLQDDSKVFKEGS-CLLADDNFVLIGSFVSFFIPLTIMVITYFLT 257

  Fly   469 IPLLRKKARFAAEHPESELFRRLGGRFTLRPQHSQQQLQMFSSFSGSNNKFLSMGDGNRNFNTEG 533
            |..|:|:|......        ||.|..|            :|||     ||
Human   258 IKSLQKEATLCVSD--------LGTRAKL------------ASFS-----FL------------- 284

  Fly   534 EMEEGGAPSRRSGVEPAERPLMQQRTASSRSMGTVSFRNVVNGTSGAAGSGRRTTGTAHSSFRFS 598

Human   285 ----------------------------------------------------------------- 284

  Fly   599 GAGIFRHSSSSPASSCHSTSTSRSSSFWRKHGGYPNLMDRLAIHLRLSLVPPSSHPNRRASVRVT 663
                       |.||..|....:.|                 ||..     |.|:..||
Human   285 -----------PQSSLSSEKLFQRS-----------------IHRE-----PGSYTGRR------ 310

  Fly   664 ISQPQLGYPTNVGGNGGRTVGATEVGAGGNGSSAGRTSNSSLGGTSLMKIATIQGPILNQSQAAG 728
                                                               |:|
Human   311 ---------------------------------------------------TMQ----------- 313

  Fly   729 GNHMVQKVKPTALKPEEPQRNKVRPFKFAFNRVATPTLNLRFLNNRSKRNSLSANAVATEQKATK 793
                                                                   :::.||||.|
Human   314 -------------------------------------------------------SISNEQKACK 323

  Fly   794 VLGLVFFTFVLCWSPFFILNIIFAACPE-CQVPEHVV----NTCLWLGYVSSTINPIIYTIFNRT 853
            |||:|||.||:.|.||||.||:...|.| |.  |.|:    |..:|:||:||.:||::||:||:|
Human   324 VLGIVFFLFVVMWCPFFITNIMAVICKESCN--EDVIGALLNVFVWIGYLSSAVNPLVYTLFNKT 386

  Fly   854 FRAAFIRLLKCNCKRSGRPLRYRSVTEGRGALSLCAPSALPLAISFQGAPLMTPSTANATPMSEF 918
            :|:||.|.::|..|.:.:||:            |...:.:| |::::.:.|......|:
Human   387 YRSAFSRYIQCQYKENKKPLQ------------LILVNTIP-ALAYKSSQLQMGQKKNS------ 432

  Fly   919 RASCRTVTDDEC 930
            :...:| ||::|
Human   433 KQDAKT-TDNDC 443

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
5-HT2A	NP_730859.1	7tm_4	299..>428	CDD:304433	57/129 (44%)
5-HT2A	NP_730859.1	7tm_1	302..>477	CDD:278431	75/175 (43%)
HTR2A	NP_000612.1	7tmA_5-HT2A	75..391	CDD:341345	140/579 (24%)
		TM helix 1	77..101	CDD:341345	10/25 (40%)
		TM helix 2	110..132	CDD:341345	14/21 (67%)
		TM helix 3	149..171	CDD:341345	8/21 (38%)
		Agonist binding. /evidence=ECO:0000250\|UniProtKB:P41595	155..160		3/4 (75%)
		DRY motif, important for ligand-induced conformation changes. /evidence=ECO:0000250\|UniProtKB:P41595	172..174		0/1 (0%)
		TM helix 4	194..210	CDD:341345	5/15 (33%)
		TM helix 5	232..255	CDD:341345	10/22 (45%)
		TM helix 6	322..344	CDD:341345	14/21 (67%)
		Agonist binding. /evidence=ECO:0000250\|UniProtKB:P41595	336..340		2/3 (67%)
		TM helix 7	359..384	CDD:341345	10/24 (42%)
		NPxxY motif, important for ligand-induced conformation changes and signaling. /evidence=ECO:0000250\|UniProtKB:P41595	376..380		2/3 (67%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	450..471
		PDZ-binding. /evidence=ECO:0000269\|PubMed:11150294, ECO:0000269\|PubMed:14988405	469..471
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C165160091
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D408957at33208
OrthoFinder	1	1.000	-	-		FOG0001488
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X759
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	5	4.850