DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment 5-HT2A and Htr2a

DIOPT Version :9

Sequence 1:	NP_730859.1	Gene:	5-HT2A / 40575	FlyBaseID:	FBgn0087012	Length:	930	Species:	Drosophila melanogaster
Sequence 2:	NP_058950.1	Gene:	Htr2a / 29595	RGDID:	61800	Length:	471	Species:	Rattus norvegicus

Alignment Length:	748	Identity:	176/748 - (23%)
Similarity:	265/748 - (35%)	Gaps:	328/748 - (43%)

- Green bases have known domain annotations that are detailed below.

  Fly   189 LREVGSAEESFSNDSLLQAFQIELGAERDGLWSFLNLSELMEPDHGFLGIMNVTNTSLDMFLPNL 253
            |.::|.....:.||     |.     .||.     |.|   |..:..:...|.||.|.:.:||.
  Rat    18 LMQLGDGPRLYHND-----FN-----SRDA-----NTS---EASNWTIDAENRTNLSCEGYLPP- 63

  Fly   254 TALNTTSTASTADMATEFAVTKSFLDYSPHGYDFLFLFVVFFIFAGGLGNILVCLAVALDRKLQN 318
            |.|                   |.|......:..|...||..:...  |||||.:||:|::||||
  Rat    64 TCL-------------------SILHLQEKNWSALLTTVVIILTIA--GNILVIMAVSLEKKLQN 107

  Fly   319 VTNYFLFSLAIADLLVSLFVMPMGAIPAFLGY-WPLGFTWCNIYVTCDVLACSSSILHMCFISLG 382
            .|||||.||||||:|:...|||:..:....|| |||....|.|::..|||..::||:|:|.|||.
  Rat   108 ATNYFLMSLAIADMLLGFLVMPVSMLTILYGYRWPLPSKLCAIWIYLDVLFSTASIMHLCAISLD 172

  Fly   383 RYMGIRNPLGSRHRSTKRLTGIKIAIVWVMAMMVSSSITVLGLVNEKNIMPEPNICVINNRAFFV 447
            ||:.|:||:.....:::....:||..||.:::.:|..|.|.||.::..:..|.: |::.:..|.:
  Rat   173 RYVAIQNPIHHSRFNSRTKAFLKIIAVWTISVGISMPIPVFGLQDDSKVFKEGS-CLLADDNFVL 236

  Fly   448 FGSLVAFYIPMLMMVTTYALTIPLLRKKARFAAEHPESELFRRLGGRFTLRPQHSQQQLQMFSSF 512
            .||.|||:||:.:||.||.|||..|:|:|                   ||
  Rat   237 IGSFVAFFIPLTIMVITYFLTIKSLQKEA-------------------TL--------------- 267

  Fly   513 SGSNNKFLSMGDGNRNFNTEGEMEEGGAPSRRSGVEPAERPLMQQRTASSRSMGTVSFRNVVNGT 577

  Rat   268 ----------------------------------------------------------------- 267

  Fly   578 SGAAGSGRRTTGTAHSSFRFSGAGIFRHSSSSPASSCHSTSTSRSSSFWRKHGGYPNLMDRLAIH 642
                                                |.|..::|:               :||
  Rat   268 ------------------------------------CVSDLSTRA---------------KLA-- 279

  Fly   643 LRLSLVPPSSHPNRRASVRVTISQPQLGYPTNVGGNGGRTVGATEVGAGGNGSSAGRTSNSSLGG 707
             ..|.:|.||..:.:...|....:|                          ||.|||.:..|
  Rat   280 -SFSFLPQSSLSSEKLFQRSIHREP--------------------------GSYAGRRTMQS--- 314

  Fly   708 TSLMKIATIQGPILNQSQAAGGNHMVQKVKPTALKPEEPQRNKVRPFKFAFNRVATPTLNLRFLN 772

  Rat   315 ----------------------------------------------------------------- 314

  Fly   773 NRSKRNSLSANAVATEQKATKVLGLVFFTFVLCWSPFFILNIIFAACPE-CQVPEHVV----NTC 832
                        ::.||||.||||:|||.||:.|.||||.||:...|.| |.  |:|:    |..
  Rat   315 ------------ISNEQKACKVLGIVFFLFVVMWCPFFITNIMAVICKESCN--ENVIGALLNVF 365

  Fly   833 LWLGYVSSTINPIIYTIFNRTFRAAFIRLLKCNCKRSGRPLRYRSVTEGRGALSLCAPSALPLAI 897
            :|:||:||.:||::||:||:|:|:||.|.::|..|.:.:||:            |...:.:| |:
  Rat   366 VWIGYLSSAVNPLVYTLFNKTYRSAFSRYIQCQYKENRKPLQ------------LILVNTIP-AL 417

  Fly   898 SFQGAPLMTPSTANATPMSEFRASCRTVTDDEC 930
            :::.:.|......|:...:|     :||  |:|
  Rat   418 AYKSSQLQVGQKKNSQEDAE-----QTV--DDC 443

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
5-HT2A	NP_730859.1	7tm_4	299..>428	CDD:304433	58/129 (45%)
5-HT2A	NP_730859.1	7tm_1	302..>477	CDD:278431	77/175 (44%)
Htr2a	NP_058950.1	7tmA_5-HT2A	75..391	CDD:341345	139/579 (24%)
		TM helix 1	77..101	CDD:341345	10/25 (40%)
		TM helix 2	110..132	CDD:341345	14/21 (67%)
		TM helix 3	149..171	CDD:341345	9/21 (43%)
		Agonist binding. /evidence=ECO:0000250\|UniProtKB:P41595	155..160		3/4 (75%)
		DRY motif, important for ligand-induced conformation changes. /evidence=ECO:0000250\|UniProtKB:P41595	172..174		0/1 (0%)
		TM helix 4	194..210	CDD:341345	5/15 (33%)
		TM helix 5	232..255	CDD:341345	11/22 (50%)
		TM helix 6	322..344	CDD:341345	14/21 (67%)
		Agonist binding. /evidence=ECO:0000250\|UniProtKB:P41595	336..340		2/3 (67%)
		TM helix 7	359..384	CDD:341345	10/24 (42%)
		NPxxY motif, important for ligand-induced conformation changes and signaling. /evidence=ECO:0000250\|UniProtKB:P41595	376..380		2/3 (67%)
		PDZ-binding. /evidence=ECO:0000250\|UniProtKB:P28223	469..471
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C166354157
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D408957at33208
OrthoFinder	1	1.000	-	-		FOG0001488
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	1	1.000	-	-		X759
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
	7	6.810