Protein Alignment 5-HT2A and Htr1a

DIOPT Version :10

• Sequence 1: NP_730859.1
• Gene: 5-HT2A / 40575
• FlyBaseID: FBgn0087012
• Length: 930
• Species: Drosophila melanogaster
• Sequence 2: NP_036717.2
• Gene: Htr1a / 24473
• RGDID: 2845
• Length: 422
• Species: Rattus norvegicus
• Alignment Length: 631
• Identity: 149/631 - (23%)
• Similarity: 229/631 - (36%)
• Gaps: 238/631 - (37%)

  Fly   257 NTTST----------ASTADMATEFAVTKSFLDYSPHGYDFLFLFVVFFIFAGGLGNILVCLAVA 311
            |||::          .|.:|:...:.|..|             |.:...||...|||..|..|:|
  Rat    11 NTTASQEPFGTGGNVTSISDVTFSYQVITS-------------LLLGTLIFCAVLGNACVVAAIA 62

  Fly   312 LDRKLQNVTNYFLFSLAIADLLVSLFVMPMGAIPAFLGYWPLGFTWCNIYVTCDVLACSSSILHM 376
            |:|.||||.||.:.|||:.||:||:.|:||.|:...|..|.||...|::::..|||.|:|||||:
  Rat    63 LERSLQNVANYLIGSLAVTDLMVSVLVLPMAALYQVLNKWTLGQVTCDLFIALDVLCCTSSILHL 127

  Fly   377 CFISLGRYMGIRNPLGSRHRSTKRLTGIKIAIVWVMAMMVSSSITVLGLVNEKNIMPEPNICVIN 441
            |.|:|.||..|.:|:...::.|.|.....|::.|::..::|.. .:||....:: ..:|:.|.|:
  Rat   128 CAIALDRYWAITDPIDYVNKRTPRRAAALISLTWLIGFLISIP-PMLGWRTPED-RSDPDACTIS 190

  Fly   442 -NRAFFVFGSLVAFYIPMLMMVTTYALTIPLLRKKARFAAEHPESELFRRLGGRFTLRPQHSQQQ 505
             :..:.::.:..|||||:|:|:..|                   ..:||  ..||.:|
  Rat   191 KDHGYTIYSTFGAFYIPLLLMLVLY-------------------GRIFR--AARFRIR------- 227

  Fly   506 LQMFSSFSGSNNKFLSMGDGNRNFNTEGEMEEGGAPSRRSGVEPAERPLMQQRTASSRSMGTVSF 570
                                    .|..::|:.||                     ..|:||.|.
  Rat   228 ------------------------KTVRKVEKKGA---------------------GTSLGTSSA 247

  Fly   571 RNVVNGTSGAAGSGRRTTGTAHSSFRFSGAGIFRHSSSSPASSCHSTSTSRSSSFWRKHGGYPNL 635
            .......:|..|||.                                        ||:
  Rat   248 PPPKKSLNGQPGSGD----------------------------------------WRR------- 265

  Fly   636 MDRLAIHLRLSLVPPSSHPNRRASVRVTISQPQLGYPTNVG----GNGGRTVGATEVGAGGNGSS 696
                            ...||           .:|.|...|    |:...|:...||
  Rat   266 ----------------CAENR-----------AVGTPCTNGAVRQGDDEATLEVIEV-------- 295

  Fly   697 AGRTSNSSLGGTSLMKIATIQGPILNQSQAAGGNHMVQKVKPTALKPEEPQRNKVRPFKFAFNRV 761
                                              |.|...|.....|.|...|...|
  Rat   296 ----------------------------------HRVGNSKEHLPLPSESGSNSYAP-------- 318

  Fly   762 ATPTLNLRFLNNRSKRNSLSAN--AVATEQKATKVLGLVFFTFVLCWSPFFILNIIFAACP-ECQ 823
                   ..|..:::||:.:..  |:|.|:|..|.||::..||:|||.||||:.::...|. .|.
  Rat   319 -------ACLERKNERNAEAKRKMALARERKTVKTLGIIMGTFILCWLPFFIVALVLPFCENSCH 376

  Fly   824 VPEHVVNTCLWLGYVSSTINPIIYTIFNRTFRAAFIRLLKCN-CKR 868
            :|..:.....||||.:|.:||:||..||:.|:.||.:::||. |:|
  Rat   377 MPALLGAIINWLGYSNSLLNPVIYAYFNKDFQNAFKKIIKCKFCRR 422

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
5-HT2A	NP_730859.1	7tm_GPCRs	298..>473	CDD:475119	64/175 (37%)
		TM helix 2	321..343	CDD:410628	12/21 (57%)
		TM helix 3	359..381	CDD:410628	11/21 (52%)
		TM helix 4	404..420	CDD:410628	3/15 (20%)
		TM helix 5	443..466	CDD:410628	7/22 (32%)
		7tm_GPCRs	<779..858	CDD:475119	31/81 (38%)
		TM helix 6	792..814	CDD:410628	12/21 (57%)
		TM helix 7	826..851	CDD:410628	9/24 (38%)
Htr1a	NP_036717.2	7tmA_5-HT1A_vertebrates	37..411	CDD:320453	138/592 (23%)
		TM helix 1	38..64	CDD:320453	10/38 (26%)
		TM helix 2	71..97	CDD:320453	13/25 (52%)
		TM helix 3	109..139	CDD:320453	15/29 (52%)
		DRY motif, important for ligand-induced conformation changes. /evidence=ECO:0000250|UniProtKB:P41595	133..135		0/1 (0%)
		TM helix 4	151..173	CDD:320453	4/22 (18%)
		TM helix 5	192..221	CDD:320453	10/49 (20%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	235..261		8/46 (17%)
		TM helix 6	338..368	CDD:320453	15/29 (52%)
		TM helix 7	379..404	CDD:320453	9/24 (38%)
		NPxxY motif, important for ligand-induced conformation changes and signaling. /evidence=ECO:0000250|UniProtKB:P41595	396..400		2/3 (67%)