DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment 5-HT2A and Htr2c

DIOPT Version :9

Sequence 1:	NP_730859.1	Gene:	5-HT2A / 40575	FlyBaseID:	FBgn0087012	Length:	930	Species:	Drosophila melanogaster
Sequence 2:	NP_032338.3	Gene:	Htr2c / 15560	MGIID:	96281	Length:	459	Species:	Mus musculus

Alignment Length:	636	Identity:	157/636 - (24%)
Similarity:	235/636 - (36%)	Gaps:	275/636 - (43%)

- Green bases have known domain annotations that are detailed below.

  Fly   247 DMFLPNLTALNTTSTASTADMATEFAVTKSFLDYSPHGYDFLFLFVVFFIFAGGLGNILVCLAVA 311
            |:.:..:.|: .|.|.:::|....|    .|.| ....:..|.:.|:..:..|  |||||.:||:
Mouse    24 DISISPVAAI-VTDTFNSSDGGRLF----QFPD-GVQNWPALSIVVIIIMTIG--GNILVIMAVS 80

  Fly   312 LDRKLQNVTNYFLFSLAIADLLVSLFVMPMGAIPAFLGY-WPLGFTWCNIYVTCDVLACSSSILH 375
            :::||.|.|||||.||||||:||.|.|||:..:.....| |||....|.::::.|||..::||:|
Mouse    81 MEKKLHNATNYFLMSLAIADMLVGLLVMPLSLLAILYDYVWPLPRYLCPVWISLDVLFSTASIMH 145

  Fly   376 MCFISLGRYMGIRNPL-GSRHRS-TKRLTGIKIAIVWVMAMMVSSSITVLGLVNEKNIMPEPNIC 438
            :|.|||.||:.||||: .||..| ||.:  :||||||.:::.||..|.|:||.:|..:......|
Mouse   146 LCAISLDRYVAIRNPIEHSRFNSRTKAI--MKIAIVWAISIGVSVPIPVIGLRDESKVFVNNTTC 208

  Fly   439 VINNRAFFVFGSLVAFYIPMLMMVTTYALTIPLLRKKARFAAEHPESELFRRLGGRFTLRPQHSQ 503
            |:|:..|.:.||.|||:||:.:||.||.|||.:||::.                  ..|...|::
Mouse   209 VLNDPNFVLIGSFVAFFIPLTIMVITYFLTIYVLRRQT------------------LMLLRGHTE 255

  Fly   504 QQLQMFSSFSGSNNKFL----SMGDGNRNFNTEGEMEEGGAPSRRSGVEPAERPLMQQRTASSRS 564
            ::|:..|.      .||    ..||           ||..||:
Mouse   256 EELRNISL------NFLKCCCKKGD-----------EEENAPN---------------------- 281

  Fly   565 MGTVSFRNVVNGTSGAAGSGRRTTGTAHSSFRFSGAGIFRHSSSSPASSCHSTSTSRSSSFWRKH 629

Mouse   282 ----------------------------------------------------------------- 281

  Fly   630 GGYPNLMDRLAIHLRLSLVPPSSHPNRRASVRVTISQPQLGYPTNVGGNGGRTVGATEVGAGGNG 694
               ||               |...|.|:
Mouse   282 ---PN---------------PDQKPRRK------------------------------------- 291

  Fly   695 SSAGRTSNSSLGGTSLMKIATIQGPILNQSQAAGGNHMVQKVKPTALKPEEPQRNKVRPFKFAFN 759

Mouse   292 ----------------------------------------------------------------- 291

  Fly   760 RVATPTLNLRFLNNRSKRNSLSANAVATEQKATKVLGLVFFTFVLCWSPFFILNIIFAACPEC-- 822
                         .:.||...:..|:..|:||:||||:|||.|::.|.||||.||:...|.:.
Mouse   292 -------------KKEKRPRGTMQAINNEKKASKVLGIVFFVFLIMWCPFFITNILSVLCGKACN 343

  Fly   823 -QVPEHVVNTCLWLGYVSSTINPIIYTIFNRTFRAAFIRLLKCNCKRSGRP 872
             ::.|.::|..:|:|||.|.|||::||:||:.:|.||.:.|:|:.|...:|
Mouse   344 QKLMEKLLNVFVWIGYVCSGINPLVYTLFNKIYRRAFSKYLRCDYKPDKKP 394

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
5-HT2A	NP_730859.1	7tm_4	299..>428	CDD:304433	66/131 (50%)
5-HT2A	NP_730859.1	7tm_1	302..>477	CDD:278431	86/177 (49%)
Htr2c	NP_032338.3	7tmA_5-HT2C	55..386	CDD:320431	146/589 (25%)
		TM helix 1	56..82	CDD:320431	10/27 (37%)
		TM helix 2	89..115	CDD:320431	17/25 (68%)
		TM helix 3	128..158	CDD:320431	13/29 (45%)
		Agonist binding. /evidence=ECO:0000250	135..140		3/4 (75%)
		DRY motif, important for ligand-induced conformation changes. /evidence=ECO:0000250	152..154		0/1 (0%)
		TM helix 4	170..193	CDD:320431	11/24 (46%)
		TM helix 5	212..241	CDD:320431	15/28 (54%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	274..302		12/258 (5%)
		TM helix 6	305..335	CDD:320431	18/29 (62%)
		Agonist binding. /evidence=ECO:0000250	325..329		2/3 (67%)
		TM helix 7	348..373	CDD:320431	12/24 (50%)
		NPxxY motif, important for ligand-induced conformation changes and signaling. /evidence=ECO:0000250	365..369		2/3 (67%)
		PDZ-binding	457..459
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C167850461
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E33208_3BHN1
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D408957at33208
OrthoFinder	1	1.000	-	-		FOG0001488
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R4934
SonicParanoid	1	1.000	-	-		X759
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
	8	7.740