Protein Alignment 5-HT2A and Htr2a

DIOPT Version :10

• Sequence 1: NP_730859.1
• Gene: 5-HT2A / 40575
• FlyBaseID: FBgn0087012
• Length: 930
• Species: Drosophila melanogaster
• Sequence 2: NP_766400.1
• Gene: Htr2a / 15558
• MGIID: 109521
• Length: 471
• Species: Mus musculus
• Alignment Length: 696
• Identity: 165/696 - (23%)
• Similarity: 248/696 - (35%)
• Gaps: 308/696 - (44%)

  Fly   240 NVTNTSLDMFLPNLTALNTTSTASTADMATEFAVTKSFLDYSPHGYDFLFLFVVFFIFAGGLGNI 304
            |.||.|.:.:||. |.|                   |.|......:..|...||..:...  |||
Mouse    51 NRTNLSCEGYLPP-TCL-------------------SILHLQEKNWSALLTTVVIILTIA--GNI 93

  Fly   305 LVCLAVALDRKLQNVTNYFLFSLAIADLLVSLFVMPMGAIPAFLGY-WPLGFTWCNIYVTCDVLA 368
            ||.:||:|::||||.|||||.||||||:|:...|||:..:....|| |||....|.:::..|||.
Mouse    94 LVIMAVSLEKKLQNATNYFLMSLAIADMLLGFLVMPVSMLTILYGYRWPLPSKLCAVWIYLDVLF 158

  Fly   369 CSSSILHMCFISLGRYMGIRNPLGSRHRSTKRLTGIKIAIVWVMAMMVSSSITVLGLVNEKNIMP 433
            .::||:|:|.|||.||:.|:||:.....:::....:||..||.:::.:|..|.|.||.::..:..
Mouse   159 STASIMHLCAISLDRYVAIQNPIHHSRFNSRTKAFLKIIAVWTISVGISMPIPVFGLQDDSKVFK 223

  Fly   434 EPNICVINNRAFFVFGSLVAFYIPMLMMVTTYALTIPLLRKKARFAAEHPESELFRRLGGRFTLR 498
            |.: |::.:..|.:.||.|||:||:.:||.||.|||..|:|:|                   ||
Mouse   224 EGS-CLLADDNFVLIGSFVAFFIPLTIMVITYFLTIKSLQKEA-------------------TL- 267

  Fly   499 PQHSQQQLQMFSSFSGSNNKFLSMGDGNRNFNTEGEMEEGGAPSRRSGVEPAERPLMQQRTASSR 563
                                                                             
Mouse   268 ----------------------------------------------------------------- 267

  Fly   564 SMGTVSFRNVVNGTSGAAGSGRRTTGTAHSSFRFSGAGIFRHSSSSPASSCHSTSTSRS--SSFW 626
                                                              |.|..::|:  |||
Mouse   268 --------------------------------------------------CVSDLSTRAKLSSF- 281

  Fly   627 RKHGGYPNLMDRLAIHLRLSLVPPSSHPNRRASVRVTISQPQLGYPTNVGGNGGRTVGATEVGAG 691
                               |.:|.||..:.:...|....:|
Mouse   282 -------------------SFLPQSSLSSEKLFQRSIHREP------------------------ 303

  Fly   692 GNGSSAGRTSNSSLGGTSLMKIATIQGPILNQSQAAGGNHMVQKVKPTALKPEEPQRNKVRPFKF 756
              ||.|||.:..|
Mouse   304 --GSYAGRRTMQS---------------------------------------------------- 314

  Fly   757 AFNRVATPTLNLRFLNNRSKRNSLSANAVATEQKATKVLGLVFFTFVLCWSPFFILNIIFAACPE 821
                                        ::.||||.||||:|||.||:.|.||||.||:...|.|
Mouse   315 ----------------------------ISNEQKACKVLGIVFFLFVVMWCPFFITNIMAVICKE 351

  Fly   822 -CQVPEHVV----NTCLWLGYVSSTINPIIYTIFNRTFRAAFIRLLKCNCKRSGRPLRYRSVTEG 881
             |.  |:|:    |..:|:||:||.:||::||:||:|:|:||.|.::|..|.:.:||:
Mouse   352 SCN--ENVIGALLNVFVWIGYLSSAVNPLVYTLFNKTYRSAFSRYIQCQYKENRKPLQ------- 407

  Fly   882 RGALSLCAPSALPLAISFQGAPLMTPSTANATPMSEFRAS-CRTVT 926
                 |...:.:| .::::.:.|......|:...:|..|: |..||
Mouse   408 -----LILVNTIP-TLAYKSSQLQVGQKKNSQEDAEPTANDCSMVT 447

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
5-HT2A	NP_730859.1	7tm_GPCRs	298..>473	CDD:475119	74/175 (42%)
		TM helix 2	321..343	CDD:410628	14/21 (67%)
		TM helix 3	359..381	CDD:410628	8/21 (38%)
		TM helix 4	404..420	CDD:410628	5/15 (33%)
		TM helix 5	443..466	CDD:410628	11/22 (50%)
		7tm_GPCRs	<779..858	CDD:475119	39/83 (47%)
		TM helix 6	792..814	CDD:410628	14/21 (67%)
		TM helix 7	826..851	CDD:410628	12/28 (43%)
Htr2a	NP_766400.1	7tmA_5-HT2A	75..391	CDD:341345	139/581 (24%)
		TM helix 1	76..102	CDD:341345	10/27 (37%)
		TM helix 2	109..135	CDD:341345	15/25 (60%)
		TM helix 3	148..178	CDD:341345	13/29 (45%)
		DRY motif, important for ligand-induced conformation changes. /evidence=ECO:0000250|UniProtKB:P41595	172..174		0/1 (0%)
		TM helix 4	190..213	CDD:341345	6/22 (27%)
		TM helix 5	231..260	CDD:341345	15/28 (54%)
		TM helix 6	316..346	CDD:341345	20/29 (69%)
		TM helix 7	359..384	CDD:341345	10/24 (42%)
		NPxxY motif, important for ligand-induced conformation changes and signaling. /evidence=ECO:0000250|UniProtKB:P41595	376..380		2/3 (67%)
		PDZ-binding. /evidence=ECO:0000250|UniProtKB:P28223	469..471