DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment eIF3a and Eif3a

DIOPT Version :9

Sequence 1:	NP_649470.2	Gene:	eIF3a / 40563	FlyBaseID:	FBgn0037249	Length:	1140	Species:	Drosophila melanogaster
Sequence 2:	NP_034253.3	Gene:	Eif3a / 13669	MGIID:	95301	Length:	1344	Species:	Mus musculus

Alignment Length:	1367	Identity:	564/1367 - (41%)
Similarity:	772/1367 - (56%)	Gaps:	251/1367 - (18%)

- Green bases have known domain annotations that are detailed below.

  Fly     1 MARYTQRPENALKRANEFIEVGKPLRALDTLQEVFRNKR---WNYAYSETVIEPLMFKYLYLCVE 62
            |..|.|||||||||||||:||||...|||.|.:|.::|:   |     :.:.||:|.|||.|||:
Mouse     1 MPAYFQRPENALKRANEFLEVGKKQPALDVLYDVMKSKKHRTW-----QKIHEPIMLKYLELCVD 60

  Fly    63 LKKSHIAKEGLFQYRNMFQLVNVNSLENVIRGYLKMAEEHTEAAQAQSSAAVAVLELDDLDNIAT 127
            |:|||:|||||:||:|:.|.||:.|||:|:|.|||:|||.||||:.:|..  .||:::|||||.|
Mouse    61 LRKSHLAKEGLYQYKNICQQVNIKSLEDVVRAYLKLAEEKTEAAKEESQQ--MVLDIEDLDNIQT 123

  Fly   128 PESILMSAVCGEDAQDRSDRTILLPWVKFLWESYCQCLELLRVNTHCEALYHDIARMAFQFCLKY 192
            |||:|:|||.|||.|||:||.:|.||||||||||.|||:|||.|:..|.||||||:.||:|||:|
Mouse   124 PESVLLSAVSGEDTQDRTDRLLLTPWVKFLWESYRQCLDLLRNNSRVERLYHDIAQQAFKFCLQY 188

  Fly   193 NRKSEFRRLCDKLRKHLEDICKSSNQTTGVSINKVETQQLCLDTRLYLLDSAIQMELWQEAYKAI 257
            .||:|||:|||.||.||..|.:..||:|.:::|..|:|.:.|:|||..|||||.|||||||:||:
Mouse   189 TRKAEFRKLCDNLRMHLSQIQRHHNQSTAINLNNPESQSMHLETRLVQLDSAISMELWQEAFKAV 253

  Fly   258 EDIHGLMALSKKTPVPKTMANYYQKLAMVFSKAGNQLFHAAALLKLFQLTRELKKNLTKDDLQRM 322
            ||||||.:||||.|.|:.|||||.|::.||.|:||.||||:.|.:|:.|:||::||||::::|||
Mouse   254 EDIHGLFSLSKKPPKPQLMANYYNKVSTVFWKSGNALFHASTLHRLYHLSREMRKNLTQEEMQRM 318

  Fly   323 AAHVLLATLSIPLPSAHPEFDRFIEADKSPLEKAQKLAVLLGLPQPPTRVSLIREVVRLNVPQLV 387
            :..|||||||||:.....:..|.::.|...:||.::||.||||..||||:.||.::||.:|.|.|
Mouse   319 STRVLLATLSIPITPERTDIARLLDMDGIIVEKQRRLATLLGLQAPPTRIGLINDMVRFSVLQYV 383

  Fly   388 SEDFRNLYNWLEVDFNPLNLCKRIQSIVDFIENGPE-NALLTPYIQSLKDVTIMRLIRQISQVYE 451
            ..:.::|||||||:||||.||:|:..:::::...|| ...|..|:..|::.||:||::|::|:|:
Mouse   384 VPEVKDLYNWLEVEFNPLKLCERVTKVLNWVREQPEKEPELQQYVPQLQNNTILRLLQQVAQIYQ 448

  Fly   452 SIKFQRLLQLASFCNIFELEKLLVESVRHNDMQIRIDHQKNSIYFGTDLTESQREYRPDGPALQS 516
            ||:|.||..|..|.:.|:||:.:|::.||.|:|:||||...::.||:||..:.||..|.||.|||
Mouse   449 SIEFSRLTSLVPFVDAFQLERAIVDAARHCDLQVRIDHTSRTLSFGSDLNYATREDAPVGPHLQS 513

  Fly   517 MPSEQIRSQLVNMSTVLTRAVSIVYPNRERDQRAKLRNQMVNHYHEIKDREHQRILQRQKIIEDR 581
            |||||||:||..||:||.:|:.::.|.....::.:.....||.|.:...:||||||.|::.||:|
Mouse   514 MPSEQIRNQLTAMSSVLAKALEVIRPAHILQEKEEQHQLAVNAYLKNSRKEHQRILARRQTIEER 578

  Fly   582 KEYIEKQNNAREEEEARRQEEESRKAKLAEQKRLEQEQEERERKRHQNEIQAIREKSLKEKVQQI 646
            ||.:|..|..||:||..::|.|.:|.:.||::||.||.:|||::|...|.:.|::|:::|:::||
Mouse   579 KERLESLNIQREKEELEQREAELQKVRKAEEERLRQEAKEREKERILQEHEQIKKKTVRERLEQI 643

  Fly   647 SQTAHGKKMLSKLDEEGIKKLDAEQIAKRESEELQREAKELQSKLKSQEKKIDYFERAKRLEEIP 711
            .:|..|.|....:|.|.:::||.:.|..::.|:|::|.||||.:||:||||||||||||||||||
Mouse   644 KKTELGAKAFKDIDIEDLEELDPDFIMAKQVEQLEKEKKELQERLKNQEKKIDYFERAKRLEEIP 708

  Fly   712 LFEKYLAEKQVKDKEFWEATEKTRIENAIAERKDAVAQQERLKRMYPDRDEFLEALKKERASLYV 776
            |.:....|::|||.:.||..|:.||.....||:.|:..:.|:.||..|||.|:..||..|.|:|.
Mouse   709 LIKSAYEEQRVKDMDLWEQQEEERITTMQLEREKALEHKNRMSRMLEDRDLFVMRLKAARQSVYE 773

  Fly   777 EKLKKFEAALEAERKKRLADRIIRRREERRQAFLREKEEERLRKEEEIRLAQAAEERAAAEARRL 841
            ||||:||..|..||..||.||..:|:|||:..:.||||||..|:.||..|.:..|...|..|:|.
Mouse   774 EKLKQFEERLAEERHSRLEDRKRQRKEERKITYYREKEEEEQRRAEEQMLKEREERERAERAKRE 838

  Fly   842 EREAEDEKRRAQYE----KQRAKE---EEAERKIKEDRDRLSRELASERERTEKDRD---TWRPR 896
            |...|.::|..:.|    |:|.:|   ||.||:.:|:| ||..:..|.::....|||   ||| :
Mouse   839 EELREYQERVKKLEEVERKKRQRELEIEERERRREEER-RLGDDPLSRKDSRWGDRDSEGTWR-K 901

  Fly   897 GGDRPSASNGGSSEWRRAAPAAS-ERNDRGGERIERGGE-RIER-GGERLER------GGERI-- 950
            |   |.|    .|||||..|... .|..|..||..|..| |:.| ||:..||      ..:||
Mouse   902 G---PEA----DSEWRRGPPEKEWRRETRDDERPHRRDEDRLRRLGGDDEERESSLRPDDDRIPR 959

  Fly   951 ---------ERGGDRDRKDNEGADS---SWRVRREPDSQRAAAPKDSGAPQSRDD---------- 993
                     .||.|.||....|.|.   |||...:....|.....|.|:.:..||
Mouse   960 RGLDDDRGPRRGPDEDRFSRRGTDDDRPSWRNADDDRPPRRIGDDDRGSWRHTDDDRPPRRGLDD 1024

  Fly   994 ---KW---------RRGGERDRDFRIDGA--------RRDRDDGPRRDRDD--GPRRDRDDERGG 1036
               .|         |||.:.||..|..||        ..|.|.||||..||  ||||..||:||.
Mouse  1025 ERGSWRTADEDRGPRRGMDDDRGPRRGGADDERSSWRNADDDRGPRRGMDDDRGPRRGLDDDRGP 1089

  Fly  1037 F-----------------------------RRADGAR---------------------------R 1045
            :                             ||.|.||                           |
Mouse  1090 WRNAAEDRISRRGADDDRGPWRNMDDDRVPRRGDDARPGPWRPFVKPGGWREKEKAREESWGPPR 1154

  Fly  1046 TDEPQ--------------------------------------------RETGG----------N 1056
            ...|.                                            |:.||          :
Mouse  1155 ESRPSEEREWDRDKEKDRDNQDREENDKDLERDRDRERDGDREDRFRRPRDEGGWRRGPAEESSS 1219

  Fly  1057 WRDAPRH--ADRETRR--PAERRD-RDVRETRG-----DQRG----SAPKEAASGGVGGNWRTA- 1106
            |||:.|.  .|||.||  ..:||| ||:|:.|.     |:||    |..:||:|      ||..
Mouse  1220 WRDSSRRDDRDREDRRRDRDDRRDLRDLRDRRDLRDDRDRRGPPLRSEREEASS------WRRTD 1278

  Fly  1107 -----------PATREEKPAAKRDQAQEKE-----------------------NKAGDDGEWTSV 1137
                       |..|...||..||:.:|:|                       |:..:|| ||:|
Mouse  1279 DRKDDRTEERDPPRRVPPPALSRDREREREREGEKEKASWRAEKDRESLRRTKNETDEDG-WTTV 1342

  Fly  1138 KR 1139
            :|
Mouse  1343 RR 1344

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
eIF3a	NP_649470.2	PAM	429..>497	CDD:214803	28/67 (42%)
Eif3a	NP_034253.3	PAM	426..498	CDD:214803	31/71 (44%)
		GBP_C	<566..685	CDD:303769	48/118 (41%)
		DUF342	<643..737	CDD:302792	46/93 (49%)
		coiled coil	650..661	CDD:293879	3/10 (30%)
		Interaction with EIF3B. /evidence=ECO:0000255\|HAMAP-Rule:MF_03000	664..835		84/170 (49%)
		coiled coil	674..685	CDD:293879	5/10 (50%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	807..844		15/36 (42%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	866..1240		97/382 (25%)
		Rubella_Capsid	882..1111	CDD:283420	70/236 (30%)
		21 X 10 AA approximate tandem repeats of [DA]-[DE]-[ED]-R-[PLIGFSV]-[RPS]-[RW]-[RL]-[GNIHT]- [DGLPTAM]	924..1133		57/208 (27%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1252..1344		23/98 (23%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C167850478
Domainoid	1	1.000	439	1.000	Domainoid score	I553
eggNOG	1	0.900	-	-		E1_KOG2072
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H2779
Inparanoid	1	1.050	921	1.000	Inparanoid score	I355
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG54611
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0003667
OrthoInspector	1	1.000	-	-		oto94578
orthoMCL	1	0.900	-	-		OOG6_102668
Panther	1	1.100	-	-	LDO	PTHR14005
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R376
SonicParanoid	1	1.000	-	-		X3031
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
	16	15.790