DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment nAChRalpha4 and Chrna6

DIOPT Version :9

Sequence 1:	NP_001097669.2	Gene:	nAChRalpha4 / 40521	FlyBaseID:	FBgn0266347	Length:	539	Species:	Drosophila melanogaster
Sequence 2:	NP_476532.2	Gene:	Chrna6 / 81721	RGDID:	69281	Length:	493	Species:	Rattus norvegicus

Alignment Length:	505	Identity:	213/505 - (42%)
Similarity:	305/505 - (60%)	Gaps:	64/505 - (12%)

- Green bases have known domain annotations that are detailed below.

  Fly    13 KRLYDDLLSNYNKLVRPVVNTTDVLKVCIKLKLSQLIDVNLKNQIMTTNLWVEQSWYDYKLRWEP 77
            ::|:..|.::||:.:|||.|.:|.:.|..:|.::||.:|:..||||.||||:...|.||:|.|:|
  Rat    35 EQLFHTLFAHYNRFIRPVENVSDPVTVHFELAITQLANVDEVNQIMETNLWLRHVWKDYRLCWDP 99

  Fly    78 KEYGGVHMLHVPSDHIWRPDIVLYNNADGNFEVTLATKATIYSEGLVEWKPPAIYKSSCEIDVEY 142
            .||.|:..|.||:|:||:|||||||||.|:|:|...|||.:..:|::.|.||||:||||.:|:.:
  Rat   100 TEYDGIETLRVPADNIWKPDIVLYNNAVGDFQVEGKTKALLKYDGVITWTPPAIFKSSCPMDITF 164

  Fly   143 FPFDEQTCVLKFGSWTYDGFKVDLRHMDEQQGSNVVAVGVDLSEFYMSVEWDILEVPAVRNEKFY 207
            ||||.|.|.|||||||||..::||.         ::...||:::|:.:.||:|::....:::..|
  Rat   165 FPFDHQNCSLKFGSWTYDKAEIDLL---------IIGSKVDMNDFWENSEWEIVDASGYKHDIKY 220

  Fly   208 TCCDEPYLDITFNITMRRKTLFYTVNIIIPCMGISFLTVLTFYLPSDSGEKVTLSISILISLHVF 272
            .||:|.|.|||::..:||..:|||:|:||||:.|||||||.||||||.||||||.||:|:||.||
  Rat   221 NCCEEIYTDITYSFYIRRLPMFYTINLIIPCLFISFLTVLVFYLPSDCGEKVTLCISVLLSLTVF 285

  Fly   273 FLLVVEIIPPTSLVVPLLGKYLIFAMILVSISICVTVVVLNVHFRSPQTHKMAPWVKRVFIDFLP 337
            .|::.|.||.||||:||:|:||:|.||.|::||.|||.|||:|:|:|.||.|..|||.:|:...|
  Rat   286 LLVITETIPSTSLVIPLVGEYLLFTMIFVTLSIVVTVFVLNIHYRTPATHTMPKWVKTMFLQVFP 350

  Fly   338 AFLFIKRPTYNFETSKLL--LKD--THACFYPYYSATNIDRLVSSGYNNSLPKEDLSQSITANGP 398
            :.|.::||   .:.:|..  :||  ||.                        |.......|
  Rat   351 SILMMRRP---LDKTKEADGVKDPKTHT------------------------KRPAKVKFT---- 384

  Fly   399 FGGSCQVHGPVPPL----THC-SSDEIAAVPDMDIGPLGMKSPILNNPAFSHSKCLPKIHKSCFC 458
                   |...|.|    .|| .|.|||....:...|....:....:|        |.:......
  Rat   385 -------HRKEPKLLKECRHCHKSSEIAPGKRLSQQPAQWVAENSEHP--------PDVEDVIDS 434

  Fly   459 VRFIAEHTKMQEDSTKVKEDWKYVAMVLDRLFLWIFTLAVVVGTAGIILQ 508
            |:||.|:.|...::.:|::||||:|||:||:|||:|.:..|.||.|:.||
  Rat   435 VQFITENMKSHNETKEVEDDWKYMAMVVDRVFLWVFIIVCVFGTVGLFLQ 484

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha4	NP_001097669.2	LIC	1..505	CDD:273305	210/500 (42%)
		Neur_chan_LBD	12..227	CDD:280998	96/213 (45%)
		Neur_chan_memb	234..505	CDD:280999	110/279 (39%)
Chrna6	NP_476532.2	LIC	34..483	CDD:273305	211/502 (42%)
		LGIC_ECD	34..240	CDD:355788	96/213 (45%)
		Cys-loop	158..172	CDD:349787	7/13 (54%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	253	1.000	Domainoid score	I1995
eggNOG	1	0.900	-	-		E1_KOG3645
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	477	1.000	Inparanoid score	I1422
OMA	1	1.010	-	-		QHG45250
OrthoDB	1	1.010	-	-		D188416at33208
OrthoFinder	1	1.000	-	-		FOG0000583
OrthoInspector	1	1.000	-	-		mtm12327
orthoMCL	1	0.900	-	-		OOG6_101158
Panther	1	1.100	-	-	O	PTHR18945
Phylome	1	0.910	-	-
SonicParanoid	1	1.000	-	-		X294
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	13	12.880