DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment nAChRalpha4 and chrna2b

DIOPT Version :9

Sequence 1:	NP_001097669.2	Gene:	nAChRalpha4 / 40521	FlyBaseID:	FBgn0266347	Length:	539	Species:	Drosophila melanogaster
Sequence 2:	XP_697298.4	Gene:	chrna2b / 568849	ZFINID:	ZDB-GENE-041001-99	Length:	550	Species:	Danio rerio

Alignment Length:	536	Identity:	238/536 - (44%)
Similarity:	318/536 - (59%)	Gaps:	74/536 - (13%)

- Green bases have known domain annotations that are detailed below.

  Fly    15 LYDDLLSNYNKLVRPVVNTTDVLKVCIKLKLSQLIDVNLKNQIMTTNLWVEQSWYDYKLRWEPKE 79
            |:..|...|||..|||.||:||:.|...|.::|||||:.|||:||||:|::|.|.||||||:|.:
Zfish    38 LFKKLFDGYNKWSRPVPNTSDVVIVKFGLSIAQLIDVDEKNQMMTTNVWLKQEWNDYKLRWKPSD 102

  Fly    80 YGGVHMLHVPSDHIWRPDIVLYNNADGNFEVTLATKATIYSEGLVEWKPPAIYKSSCEIDVEYFP 144
            |..|..:.|||:.||.|||||||||||.|.||..|||.::..|.|.|.|||||||||.|||.:||
Zfish   103 YDNVTSIRVPSELIWVPDIVLYNNADGEFAVTHMTKAHLFHTGKVRWVPPAIYKSSCSIDVTFFP 167

  Fly   145 FDEQTCVLKFGSWTYDGFKVDLRHMDEQQGSNVVAVGVDLSEFYMSVEWDILEVPAVRNEKFYTC 209
            ||:|.|.:||||||||..|:||..::..         |||.:::.|.||.|:......|.|.|.|
Zfish   168 FDQQNCKMKFGSWTYDKAKIDLERIENT---------VDLKDYWESGEWAIINAVGTYNTKKYDC 223

  Fly   210 CDEPYLDITFNITMRRKTLFYTVNIIIPCMGISFLTVLTFYLPSDSGEKVTLSISILISLHVFFL 274
            |.|.|.|||:...:||..||||:|:||||:.||.||||.||||||.|||:||.||:|:||.||.|
Zfish   224 CHEIYPDITYFFIIRRLPLFYTINLIIPCLLISCLTVLVFYLPSDCGEKITLCISVLLSLTVFLL 288

  Fly   275 LVVEIIPPTSLVVPLLGKYLIFAMILVSISICVTVVVLNVHFRSPQTHKMAPWVKRVFIDFLPAF 339
            |:.||||.||||:||:|:||:|.||.|::||.:||.|||||.|||.||||..||..||:|.:|.:
Zfish   289 LITEIIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHHRSPSTHKMPCWVHSVFLDLIPRW 353

  Fly   340 LFIKRPTYNF-ETSKLLL-----------------------------KDTHACFYPYYSATNIDR 374
            ||::||..:. ...||||                             .:..:|.:..|....:..
Zfish   354 LFMRRPAPDSRRRQKLLLLQRQVPGMTARPANASLSTSTSWFRGQNSGEDESCRHFSYKDLELGT 418

  Fly   375 LVSSGYNNSLPKEDLSQSITANGPFGGSCQVHGPVPPLTHCSSDEIAAVPDMDIG----PLGMKS 435
            |.|:          :|.|:.|:.|    |.:....|||     .:....|.:::|    .|..::
Zfish   419 LSSA----------ISLSLQASSP----CPLGLTPPPL-----QKFGPAPRLEVGTASRQLSGRA 464

  Fly   436 PIL---------NNPAFSHSKCLPKIHKSCFCVRFIAEHTKMQEDSTKVKEDWKYVAMVLDRLFL 491
            .:.         :||.|..|   |.:.::...|.:||:|.:.::....|||||||||||:||:||
Zfish   465 NVTKRLDNMIPHDNPGFPLS---PSVLQALEGVHYIADHLRAEDADFSVKEDWKYVAMVIDRIFL 526

  Fly   492 WIFTLAVVVGTAGIIL 507
            |:|.:..::||.|:.|
Zfish   527 WMFIIVCLLGTIGLFL 542

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha4	NP_001097669.2	LIC	1..505	CDD:273305	236/532 (44%)
		Neur_chan_LBD	12..227	CDD:280998	111/211 (53%)
		Neur_chan_memb	234..505	CDD:280999	120/313 (38%)
chrna2b	XP_697298.4	LIC	6..543	CDD:273305	238/536 (44%)
		Neur_chan_LBD	35..241	CDD:280998	111/211 (53%)
		Neur_chan_memb	248..541	CDD:280999	121/314 (39%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	250	1.000	Domainoid score	I2065
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	473	1.000	Inparanoid score	I1461
OMA	1	1.010	-	-		QHG45250
OrthoDB	1	1.010	-	-		D188416at33208
OrthoFinder	1	1.000	-	-		FOG0000583
OrthoInspector	1	1.000	-	-		mtm6399
orthoMCL	1	0.900	-	-		OOG6_101158
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R2381
SonicParanoid	1	1.000	-	-		X294
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	11	10.910