Protein Alignment nAChRalpha4 and chrna4b

DIOPT Version :9

• Sequence 1: NP_001097669.2
• Gene: nAChRalpha4 / 40521
• FlyBaseID: FBgn0266347
• Length: 539
• Species: Drosophila melanogaster
• Sequence 2: NP_001041528.1
• Gene: chrna4b / 556619
• ZFINID: ZDB-GENE-090505-3
• Length: 627
• Species: Danio rerio
• Alignment Length: 606
• Identity: 245/606 - (40%)
• Similarity: 329/606 - (54%)
• Gaps: 129/606 - (21%)

  Fly    13 KRLYDDLLSNYNKLVRPVVNTTDVLKVCIKLKLSQLIDVNLKNQIMTTNLWVEQSWYDYKLRWEP 77
            :||...|..|||||.|||.|.:||:.|...|.::|||||:.|||:||||:||:|.|.||||||.|
Zfish    32 ERLLQSLFINYNKLSRPVANISDVVLVHFGLSIAQLIDVDEKNQMMTTNVWVKQEWNDYKLRWNP 96

  Fly    78 KEYGGVHMLHVPSDHIWRPDIVLYNNADGNFEVTLATKATIYSEGLVEWKPPAIYKSSCEIDVEY 142
            :||..|..:.:||:.||||||||||||||:|.||..|||.::..|.::|||||||||||.|||.:
Zfish    97 EEYENVTSIRIPSEIIWRPDIVLYNNADGDFAVTHLTKAQVFYNGRIKWKPPAIYKSSCSIDVTF 161

  Fly   143 FPFDEQTCVLKFGSWTYDGFKVDLRHMDEQQGSNVVAVGVDLSEFYMSVEWDILEVPAVRNEKFY 207
            ||||:|.|.:||||||||..|:||..|         |..||..:::.|.||.|:......|.|.|
Zfish   162 FPFDQQNCKMKFGSWTYDRAKIDLISM---------ASDVDQMDYWESGEWVIINAVGTYNIKKY 217

  Fly   208 TCCDEPYLDITFNITMRRKTLFYTVNIIIPCMGISFLTVLTFYLPSDSGEKVTLSISILISLHVF 272
            .||.|.|.|||::..:||..||||:|:||||:.||.||||.|||||:..||:||.||:|:||.||
Zfish   218 ECCTEIYPDITYSFIIRRLPLFYTINLIIPCLLISCLTVLVFYLPSECAEKITLCISVLLSLTVF 282

  Fly   273 FLLVVEIIPPTSLVVPLLGKYLIFAMILVSISICVTVVVLNVHFRSPQTHKMAPWVKRVFIDFLP 337
            .||:.||||.||||:||:|:||:|.||.|::||.:||.|||||.||.:||.|..||:::|:..:|
Zfish   283 LLLITEIIPSTSLVIPLIGEYLLFTMIFVTLSIIITVFVLNVHHRSSRTHSMPHWVRQLFLHLVP 347

  Fly   338 AFLFIKRPTYN---------------------FETSKLL----------LKDTHA---------- 361
            .:||:|||..:                     |..:.:|          |..|.|
Zfish   348 RYLFMKRPPASGKRNCGKLIEMMHKQANSQSMFLRNNILDISPQSPIPHLGPTSAAQQDQVQQDS 412

  Fly   362 --------CFYP--YYSATNIDR---------LVSSGYNNS--------------------LPKE 387
                    |..|  .||....:.         ..:..|.||                    :|::
Zfish   413 TSPKPLFFCSSPSSQYSILQEENSQTPQTPVLSYTCAYTNSIFASSTTSFPDTLLGTLLHTIPQD 477

  Fly   388 DLS------QSITANGPF---------GGSCQVHGPVPPLTHCSSDEIAAVPDMDIGPLG----- 432
            ...      |..:|...|         .||||         ||.:.|..::..:|:...|
Zfish   478 GAKSTECDFQGHSAESVFMEAKESVLGAGSCQ---------HCLTSEGTSLQKLDLDEAGRCTRH 533

  Fly   433 -MKSPILNNPAFSHSKCL----------PKIHKSCFCVRFIAEHTKMQEDSTKVKEDWKYVAMVL 486
             |.|..:.:|:...|..|          ..:.|:...|::||:|.:.::....|:|||||||||:
Zfish   534 NMPSAQIFSPSSDGSTDLNAAEPVSNLSQSLLKALEGVQYIADHLRAEDSDFSVREDWKYVAMVI 598

  Fly   487 DRLFLWIFTLAVVVGTAGIIL 507
            ||:|||:|.|..::||.|:.|
Zfish   599 DRIFLWMFVLVCILGTVGLFL 619

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha4	NP_001097669.2	LIC	1..505	CDD:273305	243/602 (40%)
		Neur_chan_LBD	12..227	CDD:280998	116/213 (54%)
		Neur_chan_memb	234..505	CDD:280999	122/381 (32%)
chrna4b	NP_001041528.1	Neur_chan_LBD	31..237	CDD:280998	116/213 (54%)
		Neur_chan_memb	244..618	CDD:280999	123/382 (32%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	250	1.000	Domainoid score	I2065
eggNOG	1	0.900	-	-		E1_KOG3645
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	473	1.000	Inparanoid score	I1461
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D188416at33208
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	1	1.000	-	-		mtm6399
orthoMCL	1	0.900	-	-		OOG6_101158
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X294
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	9	8.770