Protein Alignment nAChRalpha4 and Chrna10

DIOPT Version :10

• Sequence 1: NP_001097669.2
• Gene: nAChRalpha4 / 40521
• FlyBaseID: FBgn0266347
• Length: 539
• Species: Drosophila melanogaster
• Sequence 2: NP_001074893.1
• Gene: Chrna10 / 504186
• MGIID: 3609260
• Length: 447
• Species: Mus musculus
• Alignment Length: 498
• Identity: 167/498 - (33%)
• Similarity: 254/498 - (51%)
• Gaps: 81/498 - (16%)

  Fly    12 AKRLYDDLLSNYNKLVRPVVNTTDVLKVCIKLKLSQLIDVNLKNQIMTTNLWVEQSWYDYKLRWE 76
            |.:|:.||.:||...:|||.:|...|.|.:::.|||:||::.:||::|..||:.|.|.|..|.|:
Mouse    30 AHKLFRDLFANYTSALRPVADTDQTLNVTLEVTLSQIIDMDERNQVLTLYLWIRQEWTDAYLHWD 94

  Fly    77 PKEYGGVHMLHVPSDHIWRPDIVLYNNADGNFEVTLATKATIYSEGLVEWKPPAIYKSSCEIDVE 141
            ||.||.:..:.:||..:|||||||||.||.....:.:|...:..:|.|.|..|||.:|||.:||.
Mouse    95 PKAYGDLDAIRIPSSLVWRPDIVLYNKADTQPPASASTNVVVRHDGAVRWDAPAITRSSCRVDVS 159

  Fly   142 YFPFDEQTCVLKFGSWTYDGFKVDLRHMDEQQGSNVVAVGVDLSEFYMSVEWDILEVPAVRNEKF 206
            .||||.|.|.|.|||||:.|.::|:|    .:|::     ..|::|..:|||.:|.:||.|....
Mouse   160 AFPFDAQRCGLTFGSWTHGGHQLDVR----PRGTS-----ASLADFVENVEWRVLGMPARRRVLT 215

  Fly   207 YTCCDEPYLDITFNITMRRKTLFYTVNIIIPCMGISFLTVLTFYLPSDSGEKVTLSISILISLHV 271
            |.||.|||.|:||.:.:||:...|..|:::||:.||.|..|.|:||:||||||:|.:::|::|.|
Mouse   216 YGCCSEPYPDVTFTLLLRRRAAAYVCNLLLPCVFISLLAPLAFHLPADSGEKVSLGVTVLLALTV 280

  Fly   272 FFLLVVEIIPPTSLVVPLLGKYLIFAMILVSISICVTVVVLNVHFRSPQTHKMAPWVKRVFIDFL 336
            |.|::.|.:||.. .|||:|||.:..|.:|:.|..:|::::|:|:..|..|.:..|.:.:.:..|
Mouse   281 FQLILAESMPPAE-SVPLIGKYYMATMTMVTFSTALTILIMNLHYCGPNAHPVPAWARVLLLGHL 344

  Fly   337 PAFLFIKRPTYNFETSKLLLKDTHACFYPYYSATNIDRLVSSGYNNSLPKEDLSQSITANGPFGG 401
            ...|.::             :....|                  ..|.|.|.........||..|
Mouse   345 AKGLCVR-------------ERGEPC------------------GQSKPLESAPSLQPPAGPPAG 378

  Fly   402 SCQVHGPVPPLTHCSSDEIAAVPDMDIGPLGMKSPILNNPAFSHSKCLPKIHKSCFCVRFIAEHT 466
            .|  |.|     .|                           ..|.:.|  :|.    |..||...
Mouse   379 PC--HEP-----RC---------------------------LCHQEAL--LHH----VASIAGTF 403

  Fly   467 KMQEDSTKVKEDWKYVAMVLDRLFLWIFTLAVVVGTAGIILQA 509
            :....:.:..||||.:|.|:||.||.||....:|.:..:::||
Mouse   404 RSHRAAQRRHEDWKRLARVMDRFFLGIFFCMALVMSLLVLVQA 446

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha4	NP_001097669.2	LIC	1..505	CDD:455710	165/492 (34%)
		LGIC_ECD_nAChR_proto_alpha-like	11..229	CDD:349832	92/216 (43%)
		Cys-loop	136..150	CDD:349832	8/13 (62%)
Chrna10	NP_001074893.1	LGIC_ECD	30..236	CDD:475126	92/214 (43%)
		LGIC_TM_cation	239..>324	CDD:349853	37/85 (44%)
		TM1 helix	239..261	CDD:349853	9/21 (43%)
		Neur_chan_memb	243..437	CDD:460753	70/265 (26%)
		TM2 helix	269..290	CDD:349853	7/20 (35%)
		TM3 helix	300..322	CDD:349853	6/21 (29%)