Protein Alignment nAChRalpha4 and nAChRalpha1

DIOPT Version :10

• Sequence 1: NP_001097669.2
• Gene: nAChRalpha4 / 40521
• FlyBaseID: FBgn0266347
• Length: 539
• Species: Drosophila melanogaster
• Sequence 2: NP_001262916.1
• Gene: nAChRalpha1 / 42918
• FlyBaseID: FBgn0000036
• Length: 597
• Species: Drosophila melanogaster
• Alignment Length: 551
• Identity: 314/551 - (56%)
• Similarity: 385/551 - (69%)
• Gaps: 52/551 - (9%)

  Fly     2 VHGAVAG--NPDAKRLYDDLLSNYNKLVRPVVNTTDVLKVCIKLKLSQLIDVNLKNQIMTTNLWV 64
            :|.|..|  ||||||||||||||||:|:|||.|.:|.|.|.:.|:|||||||||||||||||:||
  Fly    13 LHFATGGLANPDAKRLYDDLLSNYNRLIRPVGNNSDRLTVKMGLRLSQLIDVNLKNQIMTTNVWV 77

  Fly    65 EQSWYDYKLRWEPKEYGGVHMLHVPSDHIWRPDIVLYNNADGNFEVTLATKATIYSEGLVEWKPP 129
            ||.|.||||:|.|.:||||..|||||:|||.||||||||||||:|||:.|||.::..|.|.||||
  Fly    78 EQEWNDYKLKWNPDDYGGVDTLHVPSEHIWLPDIVLYNNADGNYEVTIMTKAILHHTGKVVWKPP 142

  Fly   130 AIYKSSCEIDVEYFPFDEQTCVLKFGSWTYDGFKVDLRHMDEQQGSNVVAVGVDLSEFYMSVEWD 194
            |||||.|||||||||||||||.:|||||||||:.|||||:.:...|:.:.||:||.::|:|||||
  Fly   143 AIYKSFCEIDVEYFPFDEQTCFMKFGSWTYDGYMVDLRHLKQTADSDNIEVGIDLQDYYISVEWD 207

  Fly   195 ILEVPAVRNEKFYTCCDEPYLDITFNITMRRKTLFYTVNIIIPCMGISFLTVLTFYLPSDSGEKV 259
            |:.||||||||||:||:||||||.||:|:||||||||||:||||:|||||:||.||||||||||:
  Fly   208 IMRVPAVRNEKFYSCCEEPYLDIVFNLTLRRKTLFYTVNLIIPCVGISFLSVLVFYLPSDSGEKI 272

  Fly   260 TLSISILISLHVFFLLVVEIIPPTSLVVPLLGKYLIFAMILVSISICVTVVVLNVHFRSPQTHKM 324
            :|.||||:||.|||||:.||||||||.||||||||:|.|:||::|:.||:.||||:||||.||:|
  Fly   273 SLCISILLSLTVFFLLLAEIIPPTSLTVPLLGKYLLFTMMLVTLSVVVTIAVLNVNFRSPVTHRM 337

  Fly   325 APWVKRVFIDFLPAFLFIKRPTY---NFETSKLLLKDTHACFYPYYSATNIDRLVS------SGY 380
            ||||:|:||..||..|.|:||..   ..:....:|.|.      |:...::|:.|:      ||
  Fly   338 APWVQRLFIQILPKLLCIERPKKEEPEEDQPPEVLTDV------YHLPPDVDKFVNYDSKRFSG- 395

  Fly   381 NNSLPKEDLSQ--SITANGPFGGSCQVHGPVPPLTHCSSDEIAAVPDMDIGPLGMKSPILNNPAF 443
            :..:|....|.  .:.|.|.....|....|:|     ||..:....|....|.|:...|  :|.
  Fly   396 DYGIPALPASHRFDLAAAGGISAHCFAEPPLP-----SSLPLPGADDDLFSPSGLNGDI--SPG- 452

  Fly   444 SHSKCLP--------------------KIHKSCFCVRFIAEHTKMQEDSTKVKEDWKYVAMVLDR 488
                |.|                    ::.|:....||||:|.|.::....|:||||||||||||
  Fly   453 ----CCPAAAAAAAADLSPTFEKPYAREMEKTIEGSRFIAQHVKNKDKFESVEEDWKYVAMVLDR 513

  Fly   489 LFLWIFTLAVVVGTAGIILQAPTLYDDRIPI 519
            :|||||.:|.|||||.||||||:|||...||
  Fly   514 MFLWIFAIACVVGTALIILQAPSLYDQSQPI 544

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha4	NP_001097669.2	LIC	1..505	CDD:455710	303/535 (57%)
		LGIC_ECD_nAChR_proto_alpha-like	11..229	CDD:349832	158/217 (73%)
		Cys-loop	136..150	CDD:349832	13/13 (100%)
nAChRalpha1	NP_001262916.1	LGIC_ECD_nAChR_proto_alpha-like	24..242	CDD:349832	158/217 (73%)
		LIC	27..530	CDD:455710	295/521 (57%)
		Cys-loop	149..163	CDD:349832	13/13 (100%)