Protein Alignment nAChRalpha4 and chrna1.2

DIOPT Version :10

• Sequence 1: NP_001097669.2
• Gene: nAChRalpha4 / 40521
• FlyBaseID: FBgn0266347
• Length: 539
• Species: Drosophila melanogaster
• Sequence 2: XP_012825723.1
• Gene: chrna1.2 / 394991
• XenbaseID: XB-GENE-5932067
• Length: 457
• Species: Xenopus tropicalis
• Alignment Length: 517
• Identity: 206/517 - (39%)
• Similarity: 293/517 - (56%)
• Gaps: 92/517 - (17%)

  Fly     4 GAVAGNPDAKRLYDDLLSNYNKLVRPVVNTTDVLKVCIKLKLSQLIDVNLKNQIMTTNLWVEQSW 68
            |.|..:....||..||.:||||:||||....|.:.|.:.|:|.|||:|:..|||::||:.::|.|
 Frog    16 GPVFSSDHETRLIGDLFANYNKVVRPVETYKDQVVVTVGLQLIQLINVDEVNQIVSTNIRLKQQW 80

  Fly    69 YDYKLRWEPKEYGGVHMLHVPSDHIWRPDIVLYNNADGNFEVTLATKATIYSEGLVEWKPPAIYK 133
            .|..|:|:|.:||||..|.:||..:|.||:||||||||:|.::..||..:...|.:.|.||||:|
 Frog    81 VDVNLKWDPAKYGGVKKLRIPSSEVWCPDLVLYNNADGDFAISKDTKILLEHTGKITWTPPAIFK 145

  Fly   134 SSCEIDVEYFPFDEQTCVLKFGSWTYDGFKVDLRHMDEQQGSNVVAVGVDLSEFYMSVEWDILEV 198
            |.|||.|.:||||:|.|.:|||:|||||..|.:....::.         |||.|..|.||.:.:.
 Frog   146 SYCEIIVTHFPFDQQNCSMKFGTWTYDGTLVVINPDRDRP---------DLSNFMASGEWMMKDY 201

  Fly   199 PAVRNEKFYTCC-DEPYLDITFNITMRRKTLFYTVNIIIPCMGISFLTVLTFYLPSDSGEKVTLS 262
            ...::..:|||| |:||||||::..::|..|::.||:||||:..||||.|.||||:|||||:|||
 Frog   202 RCWKHWVYYTCCPDKPYLDITYHFVLQRLPLYFIVNVIIPCLLFSFLTGLVFYLPTDSGEKMTLS 266

  Fly   263 ISILISLHVFFLLVVEIIPPTSLVVPLLGKYLIFAMILVSISICVTVVVLNVHFRSPQTHKMAPW 327
            ||:|:||.||.|::||:||.||..|||:|||::|.|:.|..||.:||:|:|.|.|||.||.|.||
 Frog   267 ISVLLSLTVFLLVIVELIPSTSSAVPLIGKYMLFTMVFVIASIIITVIVINTHHRSPSTHTMPPW 331

  Fly   328 VKRVFIDFLPAFLF---IKRPTYNFETSKLLLKDTHACFYPYYSATNIDRLVSSGYNNSLPKEDL 389
            |:::|||.:|..:|   :|||:                                       :|..
 Frog   332 VRKIFIDTIPNIMFFSTMKRPS---------------------------------------QEKQ 357

  Fly   390 SQSITANGPFGGSCQVHGPVPPLTHCSSDEIAAVPDMDI----GPLGMKSPILNNPAFSHSKCLP 450
            .|:..|.                            :|||    |.||..:....:||..:    |
 Frog   358 PQTTFAE----------------------------EMDISDISGKLGPAAVTYQSPALKN----P 390

  Fly   451 KIHKSCFCVRFIAEHTKMQEDSTKVKEDWKYVAMVLDRLFLWIFTLAVVVGT----AGIILQ 508
            .:..:...:::|||..|..::|.|..|:||:||||||.:.|.:|....|:||    ||.|::
 Frog   391 DVKSAIEGIKYIAETMKSDQESNKASEEWKFVAMVLDHILLAVFMTVCVIGTLAVFAGRIIE 452

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha4	NP_001097669.2	LIC	1..505	CDD:455710	204/512 (40%)
		LGIC_ECD_nAChR_proto_alpha-like	11..229	CDD:349832	98/218 (45%)
		Cys-loop	136..150	CDD:349832	9/13 (69%)
chrna1.2	XP_012825723.1	LIC	8..447	CDD:455710	203/510 (40%)
		LGIC_ECD_nAChR_A1	22..231	CDD:349815	98/217 (45%)
		Cys-loop	148..162	CDD:349815	9/13 (69%)