Protein Alignment nAChRalpha4 and Chrne

DIOPT Version :10

• Sequence 1: NP_001097669.2
• Gene: nAChRalpha4 / 40521
• FlyBaseID: FBgn0266347
• Length: 539
• Species: Drosophila melanogaster
• Sequence 2: XP_017452660.1
• Gene: Chrne / 29422
• RGDID: 2353
• Length: 547
• Species: Rattus norvegicus
• Alignment Length: 517
• Identity: 165/517 - (31%)
• Similarity: 265/517 - (51%)
• Gaps: 50/517 - (9%)

  Fly     4 GAVAGNPDAKRLYDDLLSNYNKLVRPVVNTTDVLKVCIKLKLSQLIDVNLKNQIMTTNLWVEQSW 68
            |...|..:...||..|..||:...|||....|.:.:.:|:.|:.||.:|.|.:.:||::|:...|
  Rat    69 GRSQGKNEELSLYHHLFDNYDPECRPVRRPEDTVTITLKVTLTNLISLNEKEETLTTSVWIGIEW 133

  Fly    69 YDYKLRWEPKEYGGVHMLHVPSDHIWRPDIVLYNNADGNFEVTLATKATIYSEGLVEWKPPAIYK 133
            .||:|.:...::.||.:|.|||:|:|.|:|||.||.||.|.|.......:|..|.|.|.|||||:
  Rat   134 QDYRLNFSKDDFAGVEILRVPSEHVWLPEIVLENNIDGQFGVAYDCNVLVYEGGSVSWLPPAIYR 198

  Fly   134 SSCEIDVEYFPFDEQTCVLKFGSWTYDGFKVDLRHMDEQQGSNVVAVGVDLSEFYMSVEWDILEV 198
            |:|.::|.|||||.|.|.|.|.|.||:..:|:.....:..|:.:..:.:|.:.|..:.||.|...
  Rat   199 STCAVEVTYFPFDWQNCSLIFRSQTYNAEEVEFIFAVDDDGNAINKIDIDTAAFTENGEWAIDYC 263

  Fly   199 PA-VRNEKFYTCCDEPYLDITFNITMRRKTLFYTVNIIIPCMGISFLTVLTFYLPSDS-GEKVTL 261
            |. :|:.:..:..|....|:.:.:.:|||.|||.:|||:||:.||.|.:|.::||:.: |:|.|:
  Rat   264 PGMIRHYEGGSTEDPGETDVIYTLIIRRKPLFYVINIIVPCVLISGLVLLAYFLPAQAGGQKCTV 328

  Fly   262 SISILISLHVFFLLVVEIIPPTSLVVPLLGKYLIFAMILVSISICVTVVVLNVHFRSPQTHKMAP 326
            ||::|::..||..|:.:.||.|||.|||||:||||.|::.::.:...|:||||..|:|.||..:|
  Rat   329 SINVLLAQTVFLFLIAQKIPETSLSVPLLGRYLIFVMVVATLIVMNCVIVLNVSLRTPTTHATSP 393

  Fly   327 WVKRVFIDFLPAFLFIKRPTYNFETSKLLLKDTHACFYPYYSATNIDRLVSSGYNNSLPKEDLSQ 391
            .::::.::.||..|.:..|.          :|..|. .|...|:::..|:.:  ...:.|:..|:
  Rat   394 RLRQILLELLPRLLGLSPPP----------EDPGAA-SPARRASSVGILLRA--EELILKKPRSE 445

  Fly   392 SITANGPFGGSCQVHGPVPPLTHCSSDEIAAVPDMDIGPLGMKSPILNNPAFSHSKCLPKIHKSC 456
            .:     |.|....||.......|.:...||                           |::....
  Rat   446 LV-----FEGQRHRHGTWTAAALCQNLSAAA---------------------------PEVRCCV 478

  Fly   457 FCVRFIAEHTKMQEDSTKVKEDWKYVAMVLDRLFLWIFTLAVVVGTAGIILQAPTLYDDRIP 518
            ..|.|:||.|:.||.:.:...||..:...||.:..|...:...||:..|.|..   |.:::|
  Rat   479 DAVNFVAESTRDQEATGEELSDWVRMGKALDNVCFWAALVLFSVGSTLIFLGG---YFNQVP 537

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha4	NP_001097669.2	LIC	1..505	CDD:455710	161/502 (32%)
		LGIC_ECD_nAChR_proto_alpha-like	11..229	CDD:349832	78/218 (36%)
		Cys-loop	136..150	CDD:349832	8/13 (62%)
Chrne	XP_017452660.1	LGIC_ECD_nAChR_E	102..292	CDD:349831	67/189 (35%)
		Cys-loop	201..215	CDD:349831	8/13 (62%)
		Neur_chan_memb	300..528	CDD:460753	77/272 (28%)