Protein Alignment nAChRalpha4 and Chrnb4

DIOPT Version :10

• Sequence 1: NP_001097669.2
• Gene: nAChRalpha4 / 40521
• FlyBaseID: FBgn0266347
• Length: 539
• Species: Drosophila melanogaster
• Sequence 2: NP_434693.1
• Gene: Chrnb4 / 25103
• RGDID: 2351
• Length: 495
• Species: Rattus norvegicus
• Alignment Length: 526
• Identity: 216/526 - (41%)
• Similarity: 311/526 - (59%)
• Gaps: 81/526 - (15%)

  Fly    13 KRLYDDLL--SNYNKLVRPVVNTTDVLKVCIKLKLSQLIDVNLKNQIMTTNLWVEQSWYDYKLRW 75
            ::|.||||  :.||.|:||..:::.::.:.::|.|||||.||.:.|||||::|::|.|.||:|.|
  Rat    27 EKLMDDLLNKTRYNNLIRPATSSSQLISIRLELSLSQLISVNEREQIMTTSIWLKQEWTDYRLAW 91

  Fly    76 EPKEYGGVHMLHVPSDHIWRPDIVLYNNADGNFEVTLATKATIYSEGLVEWKPPAIYKSSCEIDV 140
            ....|.||::|.:|:..:|.|||||||||||.:||::.|...:.|.|.::|.|||||||:|:|:|
  Rat    92 NSSCYEGVNILRIPAKRVWLPDIVLYNNADGTYEVSVYTNVIVRSNGSIQWLPPAIYKSACKIEV 156

  Fly   141 EYFPFDEQTCVLKFGSWTYDGFKVDLRHMDEQQGSNVVAVGVDLSEFYMSVEWDILEVPAVR--N 203
            ::||||:|.|.|||.|||||..::|:      ...:..|:   :.:|..|.||||:.:|..|  |
  Rat   157 KHFPFDQQNCTLKFRSWTYDHTEIDM------VLKSPTAI---MDDFTPSGEWDIVALPGRRTVN 212

  Fly   204 EKFYTCCDEPYLDITFNITMRRKTLFYTVNIIIPCMGISFLTVLTFYLPSDSGEKVTLSISILIS 268
            .:     |..|:|:|::..::||.||||:|:||||:.|:.|.:|.||||||.|||:||.||:|::
  Rat   213 PQ-----DPSYVDVTYDFIIKRKPLFYTINLIIPCVLITSLAILVFYLPSDCGEKMTLCISVLLA 272

  Fly   269 LHVFFLLVVEIIPPTSLVVPLLGKYLIFAMILVSISICVTVVVLNVHFRSPQTHKMAPWVKRVFI 333
            |..|.||:.:|:|||||.:||:||||:|.|:||:.||..||.|||||.|||.||.||.|||..|:
  Rat   273 LTFFLLLISKIVPPTSLDIPLIGKYLLFTMVLVTFSIVTTVCVLNVHHRSPSTHTMASWVKECFL 337

  Fly   334 DFLPAFLFIKRPTYNFETSKLLLKDTHACFYPYYSATNI---DRLVSSGYNNSLPKEDLSQSITA 395
            ..||.|||:|||  ..|.|.:.:        |:.|..::   |...:|....:.|
  Rat   338 HKLPTFLFMKRP--GLEVSLVRV--------PHPSQLHLATADTAATSALGPTSP---------- 382

  Fly   396 NGPFGGSCQVHGPVPPLTHCSSDEIAAVPDMDIGPLGMKSPILNNPAFSHSKCLPK--------- 451
            :..:|.|.....|||           |.|         ||.:     .||:..||:
  Rat   383 SNLYGSSMYFVNPVP-----------AAP---------KSAV-----SSHTAGLPRDARLRSSGR 422

  Fly   452 ----IHKSCFCVRFIAEHTKMQEDSTKVKEDWKYVAMVLDRLFLWIFTLAVVVGTAGIILQAPTL 512
                :.::...|.|||:|.:..:....|.||||:||||:||||||:|....::||.|:.|  |.|
  Rat   423 FREDLQEALEGVSFIAQHLESDDRDQSVIEDWKFVAMVVDRLFLWVFVFVCILGTMGLFL--PPL 485

  Fly   513 YDDRIP 518
            :....|
  Rat   486 FQIHAP 491

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha4	NP_001097669.2	LIC	1..505	CDD:455710	211/511 (41%)
		LGIC_ECD_nAChR_proto_alpha-like	11..229	CDD:349832	94/219 (43%)
		Cys-loop	136..150	CDD:349832	8/13 (62%)
Chrnb4	NP_434693.1	LIC	21..483	CDD:455710	213/516 (41%)
		LGIC_ECD	53..230	CDD:475126	82/190 (43%)