Protein Alignment nAChRalpha4 and Chrna5

DIOPT Version :10

• Sequence 1: NP_001097669.2
• Gene: nAChRalpha4 / 40521
• FlyBaseID: FBgn0266347
• Length: 539
• Species: Drosophila melanogaster
• Sequence 2: NP_058774.2
• Gene: Chrna5 / 25102
• RGDID: 2347
• Length: 467
• Species: Rattus norvegicus
• Alignment Length: 518
• Identity: 204/518 - (39%)
• Similarity: 289/518 - (55%)
• Gaps: 106/518 - (20%)

  Fly     4 GAVAGNPD----AKR---LYDDLLSNYNKLVRPVVNTTDVLKVCIKLKLSQLIDVNLKNQIMTTN 61
            |...|.|:    ||.   |:.||..:|.:.||||.:.:|.:|:...|.:|||:||:.|||:||||
  Rat    32 GTRGGLPELSSAAKHEDSLFRDLFEDYERWVRPVEHLSDKIKIKFGLAISQLVDVDEKNQLMTTN 96

  Fly    62 LWVEQSWYDYKLRWEPKEYGGVHMLHVPSDHIWRPDIVLYNNADGNFEVTLATKATIYSEGLVEW 126
            :|::|.|.|.||||.|.:|||:.::.||||.:|.|||||::||||.|| ..:||..:...|.|.|
  Rat    97 VWLKQEWIDVKLRWNPDDYGGIKIIRVPSDSLWIPDIVLFDNADGRFE-GASTKTVVRYNGTVTW 160

  Fly   127 KPPAIYKSSCEIDVEYFPFDEQTCVLKFGSWTYDGFKVDLRHMDEQQGSNVVAVGVDLSEFYMSV 191
            ..||.|||||.|||.:||||.|.|.:|||||||||.:||:...|:.         ||.::|:.:.
  Rat   161 TQPANYKSSCTIDVTFFPFDLQNCSMKFGSWTYDGSQVDIILEDQD---------VDRTDFFDNG 216

  Fly   192 EWDILEVPAVRNEKFYTCCDEPYLDITFNITMRRKTLFYTVNIIIPCMGISFLTVLTFYLPSDSG 256
            ||:|:.....:..:..:||..||  ||::..::|..||||:.:||||:|:|||||:.|||||:.|
  Rat   217 EWEIMSAMGSKGNRTDSCCWYPY--ITYSFVIKRLPLFYTLFLIIPCIGLSFLTVVVFYLPSNEG 279

  Fly   257 EKVTLSISILISLHVFFLLVVEIIPPTSLVVPLLGKYLIFAMILVSISICVTVVVLNVHFRSPQT 321
            ||::|..|:|:||.||.|::.||||.:|.|:||:|:||:|.||.|::||.|||..:|:|.||..|
  Rat   280 EKISLCTSVLVSLTVFLLVIEEIIPSSSKVIPLIGEYLVFTMIFVTLSIMVTVFAINIHHRSSST 344

  Fly   322 HK-MAPWVKRVFIDFLPAFLFIKRPTYNFETSKLLLKDTHACFYPYYSATNIDRLVSSGYNNSLP 385
            |. |||||:::|:..||               |||...:||           ||..:.
  Rat   345 HNAMAPWVRKIFLHKLP---------------KLLCMRSHA-----------DRYFTQ------- 376

  Fly   386 KEDLSQSITANGPFGGSCQVHGPVPPLTHCSSDEIAAVPDMDIGPLGMKSPILNNPAFSHSKCLP 450
            :|:.....             ||.      |.:.:.|..|
  Rat   377 REEAESGA-------------GPK------SRNTLEAALD------------------------- 397

  Fly   451 KIHKSCFCVRFIAEHTKMQEDSTKVKEDWKYVAMVLDRLFLWIFTLAVVVGTAGIILQAPTLY 513
                   |:|:|..|...:.|..:|.||||::|.||||:|||.|.|..::||.|:.:  |.:|
  Rat   398 -------CIRYITRHVVKENDVREVVEDWKFIAQVLDRMFLWTFLLVSIIGTLGLFV--PVIY 451

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha4	NP_001097669.2	LIC	1..505	CDD:455710	201/508 (40%)
		LGIC_ECD_nAChR_proto_alpha-like	11..229	CDD:349832	99/224 (44%)
		Cys-loop	136..150	CDD:349832	9/13 (69%)
Chrna5	NP_058774.2	LGIC_ECD	45..251	CDD:475126	98/217 (45%)
		Neur_chan_memb	257..446	CDD:460753	96/272 (35%)