Protein Alignment nAChRalpha4 and Chrna3

DIOPT Version :9

• Sequence 1: NP_001097669.2
• Gene: nAChRalpha4 / 40521
• FlyBaseID: FBgn0266347
• Length: 539
• Species: Drosophila melanogaster
• Sequence 2: NP_434692.2
• Gene: Chrna3 / 25101
• RGDID: 2345
• Length: 499
• Species: Rattus norvegicus
• Alignment Length: 538
• Identity: 230/538 - (42%)
• Similarity: 327/538 - (60%)
• Gaps: 80/538 - (14%)

  Fly     1 MVHGAVAGNPDAKRLYDDLLSNYNKLVRPVVNTTDVLKVCIKLKLSQLIDVNLKNQIMTTNLWVE 65
            |:..|.:.:....||:..|..:||:::|||.|.:..:.:..::.:|||:.|:..||||.||||::
  Rat    18 MLLPAASASEAEHRLFQYLFEDYNEIIRPVANVSHPVIIQFEVSMSQLVKVDEVNQIMETNLWLK 82

  Fly    66 QSWYDYKLRWEPKEYGGVHMLHVPSDHIWRPDIVLYNNADGNFEVTLATKATIYSEGLVEWKPPA 130
            |.|.||||:|:|.:|.||..:.||::.||:|||||||||||:|:|...|||.:...|.|.|.|||
  Rat    83 QIWNDYKLKWKPSDYQGVEFMRVPAEKIWKPDIVLYNNADGDFQVDDKTKALLKYTGEVTWIPPA 147

  Fly   131 IYKSSCEIDVEYFPFDEQTCVLKFGSWTYDGFKVDLRHMDEQQGSNVVAVG--VDLSEFYMSVEW 193
            |:||||:|||.|||||.|.|.:|||||:||..|:||           |.:|  ::|.:::.|.||
  Rat   148 IFKSSCKIDVTYFPFDYQNCTMKFGSWSYDKAKIDL-----------VLIGSSMNLKDYWESGEW 201

  Fly   194 DILEVPAVRNEKFYTCCDEPYLDITFNITMRRKTLFYTVNIIIPCMGISFLTVLTFYLPSDSGEK 258
            .|::.|..::|..|.||:|.|.|||:::.:||..||||:|:||||:.|||||||.||||||.|||
  Rat   202 AIIKAPGYKHEIKYNCCEEIYQDITYSLYIRRLPLFYTINLIIPCLLISFLTVLVFYLPSDCGEK 266

  Fly   259 VTLSISILISLHVFFLLVVEIIPPTSLVVPLLGKYLIFAMILVSISICVTVVVLNVHFRSPQTHK 323
            |||.||:|:||.||.|::.|.||.||||:||:|:||:|.||.|::||.:||.|||||:|:|.||.
  Rat   267 VTLCISVLLSLTVFLLVITETIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHYRTPTTHT 331

  Fly   324 MAPWVKRVFIDFLPAFLFIKRPT------------YNFETSKL---LLKDTHAC--FYPYYSAT- 370
            |..|||.||::.||..:|:.|||            |..|.|.|   ...|:.:|  .||....|
  Rat   332 MPTWVKAVFLNLLPRVMFMTRPTSGEGDTPKTRTFYGAELSNLNCFSRADSKSCKEGYPCQDGTC 396

  Fly   371 ---NIDRLVSSGYNNSLPKEDLSQSITANGPFGGSCQVHGPVPPLTHCSSDEIAAVPDMDIGPLG 432
               :..|:..|.::.:|.:...|:|:.|
  Rat   397 GYCHHRRVKISNFSANLTRSSSSESVDA------------------------------------- 424

  Fly   433 MKSPILNNPAFSHSKCLPKIHKSCFCVRFIAEHTKMQEDSTKVKEDWKYVAMVLDRLFLWIFTLA 497
                :|:..|.|     |:|.::...|::|||:.|.|..:.::::||||||||:||:|||:|.|.
  Rat   425 ----VLSLSALS-----PEIKEAIQSVKYIAENMKAQNVAKEIQDDWKYVAMVIDRIFLWVFILV 480

  Fly   498 VVVGTAGIILQAPTLYDD 515
            .::||||:.||.....||
  Rat   481 CILGTAGLFLQPLMARDD 498

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha4	NP_001097669.2	LIC	1..505	CDD:273305	225/526 (43%)
		Neur_chan_LBD	12..227	CDD:280998	102/216 (47%)
		Neur_chan_memb	234..505	CDD:280999	116/291 (40%)
Chrna3	NP_434692.2	LIC	24..490	CDD:273305	224/522 (43%)
		Neur_chan_LBD	29..235	CDD:280998	102/216 (47%)
		Neur_chan_memb	242..489	CDD:280999	117/292 (40%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	253	1.000	Domainoid score	I1995
eggNOG	1	0.900	-	-		E1_KOG3645
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H591
Inparanoid	1	1.050	477	1.000	Inparanoid score	I1422
OMA	1	1.010	-	-		QHG45250
OrthoDB	1	1.010	-	-		D188416at33208
OrthoFinder	1	1.000	-	-		FOG0000583
OrthoInspector	1	1.000	-	-		mtm12327
orthoMCL	1	0.900	-	-		OOG6_101158
Panther	1	1.100	-	-	O	PTHR18945
Phylome	1	0.910	-	-
SonicParanoid	1	1.000	-	-		X294
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	14	13.880