Protein Alignment nAChRalpha4 and lev-8

DIOPT Version :10

• Sequence 1: NP_001097669.2
• Gene: nAChRalpha4 / 40521
• FlyBaseID: FBgn0266347
• Length: 539
• Species: Drosophila melanogaster
• Sequence 2: NP_509932.2
• Gene: lev-8 / 191600
• WormBaseID: WBGene00002975
• Length: 531
• Species: Caenorhabditis elegans
• Alignment Length: 564
• Identity: 190/564 - (33%)
• Similarity: 297/564 - (52%)
• Gaps: 100/564 - (17%)

  Fly     6 VAGNPDAKRLYDDLLSNYNKLVRPVVNTTDVLKVCIKLKLSQLIDVNLKNQIMTTNLWVEQSWYD 70
            :..|....:|.::||::|||.||||.|.:|.|||.....|.:||||:..||::||:||:|..|||
 Worm    23 INANKHVSQLLENLLTDYNKAVRPVHNASDALKVKFGANLCRLIDVDEVNQVLTTSLWLEMQWYD 87

  Fly    71 YKLRWEPKEYGGVHMLHVPSDHIWRPDIVLYNNADGNFEVTLATKATIYSEGLVEWKPPAIYKSS 135
            .||.|.|.::|||..:|:|||.||.|||||||||||...:|:.:.|.|...|.|.|:||:||||.
 Worm    88 KKLTWNPTDWGGVEYIHIPSDQIWIPDIVLYNNADGEPHITITSLAKIDHHGRVVWQPPSIYKSF 152

  Fly   136 CEIDVEYFPFDEQTCVLKFGSWTYDGFKVDLRHMD---------EQQGSNV----VAVGVDLSEF 187
            |.|:::|||:|.|||.:|||.|:.||..:||..:.         ::|...|    :..|:.||.:
 Worm   153 CPINIKYFPYDWQTCEMKFGGWSNDGETLDLYQIPVDVDDIPQVKRQDDGVEFLYLEKGLGLSFY 217

  Fly   188 YMSVEWDILEVPAVRNEKFYT-CCDEP-YLDITFNITMRRKTLFYTVNIIIPCMGISFLTVLTF- 249
            :.|.|||:|...:.|..:.|. ||.:. |:||.:.|.:|||.:|:||.:.||||.|:.:|...|
 Worm   218 HESAEWDLLSATSSRYAQIYPGCCGQQYYIDIKYRIIIRRKAIFFTVTLTIPCMLIANVTPFVFL 282

  Fly   250 YLPSDSGEKVTLSISILISLHVFFLLVVEIIPPTSLVVPLLGKYLIFAMILVSISICVTVVVLNV 314
            .||::  .|:|.|||:|::..||:|:::::||||||.:.|:|.||:|.:::||:||.::||.:|:
 Worm   283 ILPNE--HKMTYSISVLVAFSVFYLVLIDLIPPTSLSLSLIGIYLLFTLLMVSVSIILSVVTINM 345

  Fly   315 HFRSPQTHKMAPWVKRVFIDFLPAFLFIKRPTYNFETSKLLLKDTHACFYPYYSATNIDRLVSSG 379
            :.:...:..|:.|.:.:|:..||..|.:|.
 Worm   346 YRKQAFSSGMSHWQRWLFVQRLPKLLRLKT----------------------------------- 375

  Fly   380 YNNSLPKEDLSQSITANGPFGGSCQVHGPVPPLTHCSSDEIAAVPDMDIGPLGMKSPILNNPAFS 444
            ..::|..::.|::.|   |..||..|...|...|                    .:|..|:|.|.
 Worm   376 LEHTLGPDEGSETTT---PRTGSVSVTASVTQRT--------------------LNPNNNSPVFP 417

  Fly   445 ----------------HSKC----LPKIHKSCFCVRFIAEHTKMQEDSTKVKEDWKYVAMVLDRL 489
                            ..:|    |....|....::.|:.|...|:...|:.|:|:.:::|:||:
 Worm   418 PQRLRLLSLVQMDEALKQRCAADNLDLFRKIAGHLKIISAHFHNQQMEGKITEEWQLMSLVIDRI 482

  Fly   490 FLWIFTLAVVVGTAGIILQAPTLYDDR---IPIIEQKDLDFSGT 530
            ||.:|....:......:..:|||:|||   :|.:..|.|. .||
 Worm   483 FLILFLAVSMAANIMFVYNSPTLFDDRPSLVPSVAHKPLS-GGT 525

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha4	NP_001097669.2	LIC	1..505	CDD:455710	179/534 (34%)
		LGIC_ECD_nAChR_proto_alpha-like	11..229	CDD:349832	104/232 (45%)
		Cys-loop	136..150	CDD:349832	7/13 (54%)
lev-8	NP_509932.2	LIC	10..499	CDD:455710	179/535 (33%)
		LGIC_ECD	31..261	CDD:475126	104/229 (45%)