Protein Alignment nAChRalpha4 and lgc-30

DIOPT Version :10

• Sequence 1: NP_001097669.2
• Gene: nAChRalpha4 / 40521
• FlyBaseID: FBgn0266347
• Length: 539
• Species: Drosophila melanogaster
• Sequence 2: NP_500015.3
• Gene: lgc-30 / 186551
• WormBaseID: WBGene00019069
• Length: 529
• Species: Caenorhabditis elegans
• Alignment Length: 430
• Identity: 104/430 - (24%)
• Similarity: 193/430 - (44%)
• Gaps: 64/430 - (14%)

  Fly     4 GAVAGNPDAK-----RLYDDLLSNYNKLVRPVVNTTDVLKVCIKLKLSQLIDVNLKNQIMTTNLW 63
            |.|..:|||.     ||...:.|:....: |:.:....:.|.|...|.|::|::.:|.:.|.:.:
 Worm    53 GQVIADPDADGDPHGRLATWIRSHARHHI-PLKHPHTRIDVFISAGLYQIVDLDQRNNLATVSAY 116

  Fly    64 VEQSWYDYKLRWEPKEYGGVHMLHVPSDHIWRPDIVLYNNADGNF-EVTLATKATIYSEGLVEWK 127
            ::..|.|..::|:|:.:||:..:.||...||:|:..:|::..|.. :......|.::..|||...
 Worm   117 LDVHWNDDFIKWKPEIFGGIERIFVPIKWIWKPEFYMYHSVYGRVPDYAPDASAELHYNGLVRMF 181

  Fly   128 PPAIYKSSCEIDVEYFPFDEQTCVLKFGSWTYDGFKVDLRHMDEQQGSNVVAVGVDLSEFYMSVE 192
            .....||.|.|:.:.||||.|||....|||.|....|:|....::         :.|.:||.:.|
 Worm   182 VSISSKSFCPINFKRFPFDSQTCSFSCGSWAYQSNIVNLVAAKQE---------IALEDFYDNQE 237

  Fly   193 WDILEV----PAVRNEKFYTCCDEPYLDITFNITMRRKTLFYTVNIIIPCMGISFLTVLTFYLPS 253
            |.:.:.    ...||   .:..:|.:..:...:.::|::.:|..|::.|...:|.:.|:.|:.|.
 Worm   238 WLLTDARLFNGTTRN---VSAANESFSMVYMQLDLKRQSFYYVFNLVFPTTLVSLVAVIGFHAPI 299

  Fly   254 DS----GEKVTLSISILISLHVFFLLVV-------EIIP---PTSLVVPLLGKYLIFAMILVSIS 304
            ::    ..|..|.|..|:|:.|..|::|       |.:|   .:...|||:|.|.:..:.:|||:
 Worm   300 NATGRRESKFRLGIMTLLSMSVMLLMLVGEMKFAMESVPGQRGSFSDVPLMGIYYMTLICIVSIA 364

  Fly   305 ICVTVVVLNVH---FRSPQTHKMAPW------VKRVFIDFLPAFLFIKRPTY--NFETSKLLLKD 358
            .|.:.:.:::.   .|:| :.:..||      .|::|..::|......:..|  |.....:.|..
 Worm   365 TCTSSIFVHLEKFALRNP-SWQSVPWYIRWLAAKKLFCCYVPKSFRYDKDAYSRNNNVQAISLHS 428

  Fly   359 THACFYPYYSATNIDRLVSSGYNNSLPKEDLSQSITANGP 398
            |.            ||.|:   .|.:|||.....:..:.|
 Worm   429 TP------------DRNVA---KNLIPKEINFTEVNQDAP 453

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha4	NP_001097669.2	LIC	1..505	CDD:455710	104/430 (24%)
		LGIC_ECD_nAChR_proto_alpha-like	11..229	CDD:349832	56/227 (25%)
		Cys-loop	136..150	CDD:349832	7/13 (54%)
lgc-30	NP_500015.3	Neur_chan_LBD	68..272	CDD:460752	53/216 (25%)
		LGIC_TM_cation	274..>374	CDD:349853	26/99 (26%)
		TM1 helix	274..298	CDD:349853	6/23 (26%)
		TM2 helix	310..338	CDD:349853	8/27 (30%)
		TM3 helix	352..374	CDD:349853	5/21 (24%)