Protein Alignment nAChRalpha4 and lgc-5

DIOPT Version :10

• Sequence 1: NP_001097669.2
• Gene: nAChRalpha4 / 40521
• FlyBaseID: FBgn0266347
• Length: 539
• Species: Drosophila melanogaster
• Sequence 2: NP_501411.4
• Gene: lgc-5 / 184620
• WormBaseID: WBGene00017543
• Length: 433
• Species: Caenorhabditis elegans
• Alignment Length: 526
• Identity: 108/526 - (20%)
• Similarity: 196/526 - (37%)
• Gaps: 172/526 - (32%)

  Fly    32 NTTDV---LKVCIKLKLSQLIDVNLK--------------------NQIMTTNLWVEQ---SWYD 70
            |||:|   .:|.::...::..|||:|                    :|...|.|:.::   .|||
 Worm    20 NTTNVESNTEVLLQELFNRTYDVNVKPSDTLTTVTITPNTFLLLSMDQTQETILFSQEFLLKWYD 84

  Fly    71 YKLRWEPK--EYGGVHMLHVPSDHIWRPDIVLYNNADGNFEVTLATK---------ATIYSEGLV 124
            ..|.|...  :|.. ..:.:....:|.||::        |...|:.:         |.:...|.:
 Worm    85 PILHWNRTLFDYNR-EWIKISVSRVWVPDVI--------FTKRLSVESLLDEDMQMADLRYNGEI 140

  Fly   125 EWKPPAIYKSSCEIDVEYFPFDEQTCVLKFGSWTYDGFKVDLRHMDEQQGSNVVAVGVD-----L 184
            ....||:..:||.::::.||.|.|.|.:..|||.:|...:            ||...||     .
 Worm   141 RTSLPAVVSTSCPLNIQDFPHDVQECNISMGSWAFDRTVI------------VVQSSVDEILPLR 193

  Fly   185 SEFYMSVEWDILEVPAVRNEKFYTCCDEPYLDITFNITMRRKTLFYTVNIIIPCMGISFLTVLTF 249
            ..|..:.||::|.:.|.|.:.|.......:.:||:.:|::||.::|.:.|..|...:..:|:...
 Worm   194 GRFEGNSEWELLSIRAFRTDSFEYSLGTSFSEITYTVTLQRKPVYYVLVIQAPTFILCTITIFGL 258

  Fly   250 YLP-SDSGE---KVTLSISILISLHVFFLLVVEIIPPTSLVVPLLGKYLIFAMILVSISICVTVV 310
            :.| |:..|   :|.|.:::..::.:...||.:::|..|. :||||.|:|..:.:|:.:....:|
 Worm   259 FSPNSNENERLSRVELCLNMFAAISMMLQLVSDMMPKASR-LPLLGNYIIAEIFVVTAATIAAIV 322

  Fly   311 VLNVHFRSPQTHKMAP--WVKRVFIDFLPAFLFIKRPTYNFETSKLLLKDTHACFYPYYSATNID 373
            :.:|| ....|:...|  |::
 Worm   323 IQHVH-HHVHTNATCPPKWLR-------------------------------------------- 342

  Fly   374 RLVSSGYNNSLPKEDLSQSITANGPFGGSCQVHGPVPPLTHCSSDEI----AAVPDMDIGPLGMK 434
                                         |.|      |..|..:|.    ::|..||: |..:.
 Worm   343 -----------------------------CLV------LCDCMREEFPSKTSSVSSMDV-PENII 371

  Fly   435 SPILNNPAFSHSKCLPKIHKSCFCVRFIAEHTKMQEDSTKVKED---WKYVAMVLDRLFLWIFTL 496
            :|:        |.....:||:.|.||     ..:||.| ::.|:   |..:....|.:.|.:|.:
 Worm   372 APV--------SLLKSSLHKTAFLVR-----DTLQEMS-RINENQLLWLKILDKTDLMCLILFQV 422

  Fly   497 AVVVGT 502
            |.||.|
 Worm   423 ANVVIT 428

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha4	NP_001097669.2	LIC	1..505	CDD:455710	108/526 (21%)
		LGIC_ECD_nAChR_proto_alpha-like	11..229	CDD:349832	54/238 (23%)
		Cys-loop	136..150	CDD:349832	5/13 (38%)
lgc-5	NP_501411.4	LGIC_ECD_cation	51..235	CDD:349790	43/204 (21%)
		Cys-loop	152..166	CDD:349790	5/13 (38%)
		LGIC_TM	238..>323	CDD:476813	20/85 (24%)
		TM1 helix	241..261	CDD:349853	3/19 (16%)
		TM2 helix	273..294	CDD:349853	3/20 (15%)
		TM3 helix	304..323	CDD:349853	3/18 (17%)