Protein Alignment nAChRalpha4 and lgc-25

DIOPT Version :9

• Sequence 1: NP_001097669.2
• Gene: nAChRalpha4 / 40521
• FlyBaseID: FBgn0266347
• Length: 539
• Species: Drosophila melanogaster
• Sequence 2: NP_510359.3
• Gene: lgc-25 / 181525
• WormBaseID: WBGene00010994
• Length: 502
• Species: Caenorhabditis elegans
• Alignment Length: 536
• Identity: 111/536 - (20%)
• Similarity: 226/536 - (42%)
• Gaps: 92/536 - (17%)

  Fly     6 VAGNPDAKRLYDDLLSNYNKLVRPVVNTTDVLKVCIKLKLSQL--IDVNLKNQIMTTNLWVEQSW 68
            |.|....::|...|:..||..||||.:...|.:|.:.|.::.:  :|.:.:..::..::|.  :|
 Worm    21 VCGLSKEQQLEKQLMVKYNYKVRPVKSEATVTQVNVFLSIAHVEKVDEHEQTALVHGHIWA--TW 83

  Fly    69 YDYKLRWEPKEYGGVHMLHVPSDHIWRPDIVLYNNADGN-FEVTL-ATKATIYSEGLVEWKPPAI 131
            .|..::|:.|: ..|..|.:.:..:|:|.:.|||:|.|| :.:.: |..||:||.|.| |.....
 Worm    84 IDEYMQWDAKK-ENVTKLTISAHSLWQPALALYNSARGNSWHLYMGAMPATVYSTGKV-WSTGTF 146

  Fly   132 -YKSSCEIDVEYFPFDEQTCVLKFGSWTYDGFKVDLR--HMDEQQGSNVVAVG---VDLSEFYMS 190
             :..:|:.|...:|||:|:|.:....|.|:..:|:|.  ::.::.|...:.:.   ::.|:....
 Worm   147 SFYVTCQFDFTNWPFDQQSCPIVIADWVYNLNQVNLSDPYILKEYGKPSIRLSYDPIEKSDKRHV 211

  Fly   191 VEWDIL----------------EVPAVRNEKFYTCCDEPYLDITFNITMRRKTLFYTVNIIIPCM 239
            ..|:::                |.|....|.:::.       :.|.|.:||...:::::|:.|..
 Worm   212 AGWEVMDAWKKHCYWGPKGCKEEQPEGEAEWYWSL-------LEFGIILRRHLPYFSLSILFPMA 269

  Fly   240 GISFLTVLTFYLPSDSGEKVTLSISILISLHVFFLLVVEIIPPTSLVVPLLGKYLIFAMILVSIS 304
            |.|.:.:|.|::.:.|....|:.::|::..:....|:.: :||.|...|.:.......::|.::.
 Worm   270 GTSLILLLCFWIETHSICFFTIMLNIILQSYYGSSLLAK-MPPGSGRTPKIVHLYNMNLLLSAVQ 333

  Fly   305 ICVTVVVLNVHFRSPQTHKMAPWVKRVFIDFLPAFLFIKRPTYNFETSKLLLK-----DTHACFY 364
            ..:.|....:..|.|         |:..:|.  .|..|..| ..|...||..:     |....|.
 Worm   334 FVLIVFSEFLRLRLP---------KKFELDL--GFDIIGVP-QRFGVDKLFTQKGLSFDPQELFS 386

  Fly   365 PYYSATNIDRLVSSGYNNSLPKEDLSQSITANGPFGGSCQVHGPVPPLTHCSSDEIAAVPDMDIG 429
            ..||...::.::..|..:|      |.|:....|      :|..:......|...:.    :||
 Worm   387 NVYSTRTLEEILEQGGGSS------STSVGIGNP------LHEDITSFQKSSDSSVL----IDI- 434

  Fly   430 PLGMKSPILNNP---AFSHSKCLPKIHKSCFCVRFIAEHTKMQEDSTKVKEDWKYVAMVLDRLFL 491
                  |:.:.|   :.|.|:.|.....|       .|.|::.|:    .|:.:.:.:.|..:..
 Worm   435 ------PMTSEPQSFSASTSELLSSEPSS-------NEETQISEE----PEEERILDVQLRHIKR 482

  Fly   492 WIFTLAVVVGTAGIIL 507
            ::|.|.:.:.....:|
 Worm   483 YVFCLVIFIHIIAFLL 498

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha4	NP_001097669.2	LIC	1..505	CDD:273305	110/532 (21%)
		Neur_chan_LBD	12..227	CDD:280998	56/240 (23%)
		Neur_chan_memb	234..505	CDD:280999	52/278 (19%)
lgc-25	NP_510359.3	LGIC_ECD_cation	52..256	CDD:349790	46/214 (21%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG3645
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	1	1.100	-	-	O	PTHR18945
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	3	2.910