Protein Alignment nAChRalpha4 and lgc-4

DIOPT Version :10

• Sequence 1: NP_001097669.2
• Gene: nAChRalpha4 / 40521
• FlyBaseID: FBgn0266347
• Length: 539
• Species: Drosophila melanogaster
• Sequence 2: NP_509556.4
• Gene: lgc-4 / 181155
• WormBaseID: WBGene00017580
• Length: 635
• Species: Caenorhabditis elegans
• Alignment Length: 552
• Identity: 160/552 - (28%)
• Similarity: 273/552 - (49%)
• Gaps: 68/552 - (12%)

  Fly     2 VHGAVAGNPDAKRLYDDLLSN--YNKLVRPVVNTTDVLKVCIKLKLSQLIDVNLKNQIMTTNLWV 64
            :.|::.|..:|  ||..||.:  |.|.|||.::.:....|.....|:|:::::.:||.:||..|:
 Worm   103 IKGSIDGTEEA--LYRSLLDHTVYEKDVRPCIHHSQPTNVTFGFLLNQIVEMDERNQALTTRSWL 165

  Fly    65 EQSWYDYKLRWEPKEYGGVHMLHVPSDHIWRPDIVLYNNADGNFEVTL-ATKATIYSEGLVEWKP 128
            ..:|.|.:|.|....:..:..:::|...||:|||:|.|||...:..:| :|...:.|:|.|.|..
 Worm   166 NINWMDPRLSWNESLWSEIKAIYIPHARIWKPDIILVNNAIREYYASLVSTDVMVTSDGNVTWLF 230

  Fly   129 PAIYKSSCEIDVEYFPFDEQTCVLKFGSWTYDGFKVDLRHMDEQQGSNVVAVGVDLSEFYMSVEW 193
            .|:::|||.|.|.|:|||:|.|.|||.||::|..:::| .::..:|        |||.:..:.|:
 Worm   231 SALFRSSCPIRVRYYPFDDQQCDLKFASWSHDITEINL-GLNTDKG--------DLSSYMNNSEF 286

  Fly   194 DILEVPAVRNEKFY---TCCDEPYLDITFNITMRRKTLFYTVNIIIPCMGISFLTVLTFYLPSDS 255
            |::::.|||....:   |..|.|.  |...|.|.|:.|||..|.|:||:.||.:.||.|.:|.::
 Worm   287 DLVDMTAVREVVTFPSDTNSDWPI--IVIRIHMHRRPLFYVFNHIVPCVLISSMAVLGFLMPPET 349

  Fly   256 GEKVTLSISILISLHVFFLLVVEIIPPTSLVVPLLGKYLIFAMILVSISICVTVVVLNVHFR--S 318
            |||:.:.|:.|:|:.|:...:.|.|||||..|||:|.|.:.::::|.::.||.|:.||:|..  :
 Worm   350 GEKINMIITTLLSMGVYLQSITESIPPTSEGVPLIGMYYVSSLLMVCLATCVNVITLNMHRNGAA 414

  Fly   319 PQTHKMAPWVKRVFIDFLPAF--LFIKRPTYNFETSKLLLKDTHACFYPYYSATNIDRLVSSGYN 381
            .|...:..|:::..:.:|..|  :.|:.|     .|..|||.:.:      ..:.|.|   |...
 Worm   415 NQGRHVPAWMQKWILGYLATFMRMSIREP-----DSIALLKASQS------KKSTIRR---SSIL 465

  Fly   382 NSLPKEDLSQSITANGPFGGSCQVHGPVPPLTHCSSDEIAAVPDMDIGPLGMKSPILNNPAFSHS 446
            ..|.:.....::.|......:.:....:.||.|..::.|.:....|..|  |....:.....|.|
 Worm   466 RDLKRVKNMSNVRAKSKEQNANRECECMDPLVHIYAESIMSCLAADTKP--MNGSTIREDFASES 528

  Fly   447 KCL---------PKIHKSC------FCVRF----------IAEHTKMQE---DSTKVKEDWKYVA 483
            ..|         |:|..|.      |..||          :.:|...:|   :.::::..|:.:|
 Worm   529 TFLGRVVSDGIMPRISASSNSVLTEFETRFRRILKRVYRSLQQHEIREEILDERSRIQWQWQQLA 593

  Fly   484 MVLDRLFLWIFTLAVVVGTAGIILQAPTLYDD 515
            .|:|||.|.:|..|.:. |...:|..|..|.|
 Worm   594 SVVDRLLLCLFCTATLF-TIICLLIVPVAYRD 624

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha4	NP_001097669.2	LIC	1..505	CDD:455710	156/540 (29%)
		LGIC_ECD_nAChR_proto_alpha-like	11..229	CDD:349832	73/223 (33%)
		Cys-loop	136..150	CDD:349832	8/13 (62%)
lgc-4	NP_509556.4	LGIC_ECD_nAChR	138..320	CDD:349798	62/192 (32%)
		Cys-loop	238..252	CDD:349798	8/13 (62%)
		LGIC_TM_cation	322..>409	CDD:349853	37/86 (43%)
		TM1 helix	322..346	CDD:349853	12/23 (52%)
		TM2 helix	354..375	CDD:349853	5/20 (25%)
		TM3 helix	386..408	CDD:349853	6/21 (29%)