Protein Alignment nAChRalpha4 and unc-63

DIOPT Version :9

• Sequence 1: NP_001097669.2
• Gene: nAChRalpha4 / 40521
• FlyBaseID: FBgn0266347
• Length: 539
• Species: Drosophila melanogaster
• Sequence 2: NP_491533.2
• Gene: unc-63 / 172150
• WormBaseID: WBGene00006797
• Length: 502
• Species: Caenorhabditis elegans
• Alignment Length: 529
• Identity: 265/529 - (50%)
• Similarity: 341/529 - (64%)
• Gaps: 77/529 - (14%)

  Fly     9 NPDAKRLYDDLLSNYNKLVRPVVNTTDVLKVCIKLKLSQLIDVNLKNQIMTTNLWVEQSWYDYKL 73
            |.||.||::||:::||||||||....:.|.|..|||||||:||:.||||||||:|::.||.||||
 Worm    24 NRDANRLFEDLIADYNKLVRPVSENGETLVVTFKLKLSQLLDVHEKNQIMTTNVWLQHSWMDYKL 88

  Fly    74 RWEPKEYGGVHMLHVPSDHIWRPDIVLYNNADGNFEVTLATKATIYSEGLVEWKPPAIYKSSCEI 138
            ||:|.|||||.:|:||||.||.||:|||||||||::||:.|||.:...|.|||.|||||||.|:|
 Worm    89 RWDPVEYGGVEVLYVPSDTIWLPDVVLYNNADGNYQVTIMTKAKLTYNGTVEWAPPAIYKSMCQI 153

  Fly   139 DVEYFPFDEQTCVLKFGSWTYDGFKVDLRHMDEQ---------QGSN--------VVAVGVDLSE 186
            |||:||||.|.|.:|||||||.|.:|||:|.|:.         :|.:        ||..|:|||:
 Worm   154 DVEFFPFDRQQCEMKFGSWTYGGLEVDLQHRDKHLEKEIEEDVEGVDGPTKEIVWVVDRGIDLSD 218

  Fly   187 FYMSVEWDILEVPAVRNEKFYTCCDEPYLDITFNITMRRKTLFYTVNIIIPCMGISFLTVLTFYL 251
            :|.|||||||.||..|:.|.|.||:.|::|||:.|.:||||||||||:|.|.:||||||.|.|||
 Worm   219 YYPSVEWDILNVPGKRHSKRYPCCESPFIDITYEIHLRRKTLFYTVNLIFPSVGISFLTALVFYL 283

  Fly   252 PSDSGEKVTLSISILISLHVFFLLVVEIIPPTSLVVPLLGKYLIFAMILVSISICVTVVVLNVHF 316
            |||.|||::|.|||||||.|||||:|||||.||||:||:||||:|.|:||::|:.||||.||||:
 Worm   284 PSDGGEKISLCISILISLTVFFLLLVEIIPSTSLVIPLIGKYLLFTMVLVTLSVVVTVVTLNVHY 348

  Fly   317 RSPQTHKMAPWVKRVFIDFLPAFLFIKRPTYNFETSKLLLK-DTHACFYPYYSATNIDRLVSSGY 380
            |||.||.|..|:||:|:||||.:|.:.||......||...| |:.|..:
 Worm   349 RSPTTHTMPKWMKRLFVDFLPKYLLMTRPQPPGHHSKPNRKFDSRASTF---------------- 397

  Fly   381 NNSLPKEDLSQSITANGPFGGSCQVHGPVPPLTHCSSDEIAAVPDMDIGPLGMKSPILNNPAFSH 445
                       ||..|...|.:.:                            :.||.||:.....
 Worm   398 -----------SIGVNHVLGQNSE----------------------------LLSPGLNSNREES 423

  Fly   446 SKCLPK----IHKSCFCVRFIAEHTKMQEDSTKVKEDWKYVAMVLDRLFLWIFTLAVVVGTAGII 506
            |..||:    :..:...|.:||:|.|.:||..:|.|||||:::|:||:||..||.|...||..||
 Worm   424 SFTLPRDNSPVRSAVESVAYIADHLKNEEDDKQVIEDWKYISVVMDRIFLITFTFACAFGTVVII 488

  Fly   507 LQAPTLYDD 515
            .:||::||:
 Worm   489 ARAPSIYDN 497

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha4	NP_001097669.2	LIC	1..505	CDD:273305	259/517 (50%)
		Neur_chan_LBD	12..227	CDD:280998	134/231 (58%)
		Neur_chan_memb	234..505	CDD:280999	115/275 (42%)
unc-63	NP_491533.2	LIC	6..487	CDD:273305	259/517 (50%)
		Neur_chan_LBD	29..259	CDD:280998	133/229 (58%)
		Neur_chan_memb	266..487	CDD:280999	115/275 (42%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	297	1.000	Domainoid score	I784
eggNOG	1	0.900	-	-		E1_KOG3645
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	544	1.000	Inparanoid score	I631
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG45250
OrthoDB	1	1.010	-	-		D188416at33208
OrthoFinder	1	1.000	-	-		FOG0000583
OrthoInspector	1	1.000	-	-		otm14483
orthoMCL	1	0.900	-	-		OOG6_101158
Panther	1	1.100	-	-	O	PTHR18945
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R2381
SonicParanoid	1	1.000	-	-		X294
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
	14	13.870