Protein Alignment nAChRalpha4 and unc-63

DIOPT Version :10

• Sequence 1: NP_001097669.2
• Gene: nAChRalpha4 / 40521
• FlyBaseID: FBgn0266347
• Length: 539
• Species: Drosophila melanogaster
• Sequence 2: NP_491533.2
• Gene: unc-63 / 172150
• WormBaseID: WBGene00006797
• Length: 502
• Species: Caenorhabditis elegans
• Alignment Length: 529
• Identity: 265/529 - (50%)
• Similarity: 341/529 - (64%)
• Gaps: 77/529 - (14%)

  Fly     9 NPDAKRLYDDLLSNYNKLVRPVVNTTDVLKVCIKLKLSQLIDVNLKNQIMTTNLWVEQSWYDYKL 73
            |.||.||::||:::||||||||....:.|.|..|||||||:||:.||||||||:|::.||.||||
 Worm    24 NRDANRLFEDLIADYNKLVRPVSENGETLVVTFKLKLSQLLDVHEKNQIMTTNVWLQHSWMDYKL 88

  Fly    74 RWEPKEYGGVHMLHVPSDHIWRPDIVLYNNADGNFEVTLATKATIYSEGLVEWKPPAIYKSSCEI 138
            ||:|.|||||.:|:||||.||.||:|||||||||::||:.|||.:...|.|||.|||||||.|:|
 Worm    89 RWDPVEYGGVEVLYVPSDTIWLPDVVLYNNADGNYQVTIMTKAKLTYNGTVEWAPPAIYKSMCQI 153

  Fly   139 DVEYFPFDEQTCVLKFGSWTYDGFKVDLRHMDEQ---------QGSN--------VVAVGVDLSE 186
            |||:||||.|.|.:|||||||.|.:|||:|.|:.         :|.:        ||..|:|||:
 Worm   154 DVEFFPFDRQQCEMKFGSWTYGGLEVDLQHRDKHLEKEIEEDVEGVDGPTKEIVWVVDRGIDLSD 218

  Fly   187 FYMSVEWDILEVPAVRNEKFYTCCDEPYLDITFNITMRRKTLFYTVNIIIPCMGISFLTVLTFYL 251
            :|.|||||||.||..|:.|.|.||:.|::|||:.|.:||||||||||:|.|.:||||||.|.|||
 Worm   219 YYPSVEWDILNVPGKRHSKRYPCCESPFIDITYEIHLRRKTLFYTVNLIFPSVGISFLTALVFYL 283

  Fly   252 PSDSGEKVTLSISILISLHVFFLLVVEIIPPTSLVVPLLGKYLIFAMILVSISICVTVVVLNVHF 316
            |||.|||::|.|||||||.|||||:|||||.||||:||:||||:|.|:||::|:.||||.||||:
 Worm   284 PSDGGEKISLCISILISLTVFFLLLVEIIPSTSLVIPLIGKYLLFTMVLVTLSVVVTVVTLNVHY 348

  Fly   317 RSPQTHKMAPWVKRVFIDFLPAFLFIKRPTYNFETSKLLLK-DTHACFYPYYSATNIDRLVSSGY 380
            |||.||.|..|:||:|:||||.:|.:.||......||...| |:.|..:
 Worm   349 RSPTTHTMPKWMKRLFVDFLPKYLLMTRPQPPGHHSKPNRKFDSRASTF---------------- 397

  Fly   381 NNSLPKEDLSQSITANGPFGGSCQVHGPVPPLTHCSSDEIAAVPDMDIGPLGMKSPILNNPAFSH 445
                       ||..|...|.:.:                            :.||.||:.....
 Worm   398 -----------SIGVNHVLGQNSE----------------------------LLSPGLNSNREES 423

  Fly   446 SKCLPK----IHKSCFCVRFIAEHTKMQEDSTKVKEDWKYVAMVLDRLFLWIFTLAVVVGTAGII 506
            |..||:    :..:...|.:||:|.|.:||..:|.|||||:::|:||:||..||.|...||..||
 Worm   424 SFTLPRDNSPVRSAVESVAYIADHLKNEEDDKQVIEDWKYISVVMDRIFLITFTFACAFGTVVII 488

  Fly   507 LQAPTLYDD 515
            .:||::||:
 Worm   489 ARAPSIYDN 497

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha4	NP_001097669.2	LIC	1..505	CDD:455710	259/517 (50%)
		LGIC_ECD_nAChR_proto_alpha-like	11..229	CDD:349832	137/234 (59%)
		Cys-loop	136..150	CDD:349832	10/13 (77%)
unc-63	NP_491533.2	LIC	6..487	CDD:455710	259/517 (50%)
		LGIC_ECD_nAChR_proto_alpha-like	26..261	CDD:349832	137/234 (59%)
		Cys-loop	151..165	CDD:349832	10/13 (77%)