DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment nAChRalpha4 and Chrnb3

DIOPT Version :9

Sequence 1:	NP_001097669.2	Gene:	nAChRalpha4 / 40521	FlyBaseID:	FBgn0266347	Length:	539	Species:	Drosophila melanogaster
Sequence 2:	NP_598281.1	Gene:	Chrnb3 / 171131	RGDID:	621544	Length:	464	Species:	Rattus norvegicus

Alignment Length:	519	Identity:	226/519 - (43%)
Similarity:	306/519 - (58%)	Gaps:	93/519 - (17%)

- Green bases have known domain annotations that are detailed below.

  Fly     5 AVAGNPDAKRLYDDLLSNYNKLVRPVVNTTDVLKVCIKLKLSQLIDVNLKNQIMTTNLWVEQSWY 69
            :||.:.||  |...|...|.|.||||:|::|::||...||:|||:||:.|||:||||:|::|.|.
  Rat    30 SVAEHEDA--LLRHLFQGYQKWVRPVLNSSDIIKVYFGLKISQLVDVDEKNQLMTTNVWLKQEWT 92

  Fly    70 DYKLRWEPKEYGGVHMLHVPSDHIWRPDIVLYNNADGNFEVTLATKATIYSEGLVEWKPPAIYKS 134
            |.||||.|:||||::.:.|||:.:|.|||||:.||||.||.:|.|||.:.|.|.|.|.|||.|||
  Rat    93 DQKLRWNPEEYGGINSIKVPSESLWLPDIVLFENADGRFEGSLMTKAIVKSSGTVSWTPPASYKS 157

  Fly   135 SCEIDVEYFPFDEQTCVLKFGSWTYDGFKVDLRHMDEQQGSNVVAVGVDLSEFYMSVEWDILEVP 199
            ||.:||.:||||.|.|.:|||||||||..|||..::|         .||..:|:.:.||:||...
  Rat   158 SCTMDVTFFPFDRQNCSMKFGSWTYDGTMVDLILINE---------NVDRKDFFDNGEWEILNAK 213

  Fly   200 AV---RNEKFYTCCDEPYLDITFNITMRRKTLFYTVNIIIPCMGISFLTVLTFYLPSDSGEKVTL 261
            .:   |.|.||:     |..:|::..:||..||||:.:||||:|:||||||.||||||.|||::|
  Rat   214 GMKGNRREGFYS-----YPFVTYSFVLRRLPLFYTLFLIIPCLGLSFLTVLVFYLPSDEGEKLSL 273

  Fly   262 SISILISLHVFFLLVVEIIPPTSLVVPLLGKYLIFAMILVSISICVTVVVLNVHFRSPQT-HKMA 325
            |.|:|:||.||.|::.||||.:|.|:||:|:||:|.||.|::||.|||.|:|||.||..| |.||
  Rat   274 STSVLVSLTVFLLVIEEIIPSSSKVIPLIGEYLLFIMIFVTLSIIVTVFVINVHHRSSSTYHPMA 338

  Fly   326 PWVKRVFIDFLPAFLFIKRPTYNFETSKLLLKDTHACFYPYYSATNIDRLVSSGYNNSLPKEDLS 390
            |||||:|:..||.:|.:|.|                          :||.       |.|  |..
  Rat   339 PWVKRLFLQRLPRWLCMKDP--------------------------MDRF-------SFP--DGK 368

  Fly   391 QSITANGPFGGSCQVHGPVP---PLTHCSSDEIAAVPDMDIGPLGMKSPILNNPAFSHSKCLPKI 452
            :|.||         |.|.|.   ..|..|..|...|                  ||        :
  Rat   369 ESDTA---------VRGKVSGKRKQTPASDGERVLV------------------AF--------L 398

  Fly   453 HKSCFCVRFIAEHTKMQEDSTKVKEDWKYVAMVLDRLFLWIFTLAVVVGTAGIILQAPTLYDDR 516
            .|:...:|:|:.|.|.:...::|.:|||:||.||||:|||:|.:|.|:|:..|.:.|..::..|
  Rat   399 EKASESIRYISRHVKKEHFISQVVQDWKFVAQVLDRIFLWLFLIASVLGSILIFIPALKMWIHR 462

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha4	NP_001097669.2	LIC	1..505	CDD:273305	223/506 (44%)
		Neur_chan_LBD	12..227	CDD:280998	107/217 (49%)
		Neur_chan_memb	234..505	CDD:280999	109/274 (40%)
Chrnb3	NP_598281.1	LIC	29..455	CDD:273305	224/510 (44%)
		Neur_chan_LBD	35..239	CDD:280998	108/219 (49%)
		Neur_chan_memb	246..452	CDD:280999	109/275 (40%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	253	1.000	Domainoid score	I1995
eggNOG	1	0.900	-	-		E1_KOG3645
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG45250
OrthoDB	1	1.010	-	-		D188416at33208
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	1	1.000	-	-		mtm12327
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	7	6.830