Protein Alignment nAChRalpha4 and Htr3a

DIOPT Version :10

• Sequence 1: NP_001097669.2
• Gene: nAChRalpha4 / 40521
• FlyBaseID: FBgn0266347
• Length: 539
• Species: Drosophila melanogaster
• Sequence 2: NP_038589.2
• Gene: Htr3a / 15561
• MGIID: 96282
• Length: 489
• Species: Mus musculus
• Alignment Length: 510
• Identity: 142/510 - (27%)
• Similarity: 230/510 - (45%)
• Gaps: 93/510 - (18%)

  Fly    10 PDAKRLYDDLLSNYNKLVRPVVNTTDVLKVCIKLKLSQLIDVNLKNQIMTTNLWVEQSWYDYKLR 74
            |...||.|.||:||.|.||||.:......|.|.:.:..:::|:.|||::||.:|..|.|.|..|:
Mouse    37 PALLRLSDHLLANYKKGVRPVRDWRKPTTVSIDVIMYAILNVDEKNQVLTTYIWYRQYWTDEFLQ 101

  Fly    75 WEPKEYGGVHMLHVPSDHIWRPDIVLYNNADGNFEVTLATKATIYSEGLVEWKPPAIYKSSCEID 139
            |.|:::..|..|.:|:|.||.|||::....|......: ....::..|.|:...|....::|.:|
Mouse   102 WTPEDFDNVTKLSIPTDSIWVPDILINEFVDVGKSPNI-PYVYVHHRGEVQNYKPLQLVTACSLD 165

  Fly   140 VEYFPFDEQTCVLKFGSW--TYDGFKVDLRHMDEQQGSNVVAVGVDLSEFYMSVEWDILEV-PAV 201
            :..||||.|.|.|.|.||  |.....:.|....|:       |..|.|.|....||::||| |..
Mouse   166 IYNFPFDVQNCSLTFTSWLHTIQDINITLWRSPEE-------VRSDKSIFINQGEWELLEVFPQF 223

  Fly   202 RNEKFYTCCDEPYLDITFNITMRRKTLFYTVNIIIPCMGISFLTVLTFYLPSDSGEKVTLSISIL 266
            :  :|.......|.::.|.:.:||:.|||.|::::|.:.:..:.::.|.||.||||:|:..|::|
Mouse   224 K--EFSIDISNSYAEMKFYVIIRRRPLFYAVSLLLPSIFLMVVDIVGFCLPPDSGERVSFKITLL 286

  Fly   267 ISLHVFFLLVVEIIPPTSLVVPLLGKYLIFAMILVSISICVTVVVLNVHFRSPQTHKMAPWVKRV 331
            :...||.::|.:.:|.|::..||:|.|.:..|.|:.||:..|:.::.:..:......:..|::.:
Mouse   287 LGYSVFLIIVSDTLPATAIGTPLIGVYFVVCMALLVISLAETIFIVRLVHKQDLQRPVPDWLRHL 351

  Fly   332 FIDFLPAFLFI------KRPTYNFETSKLLLKDTHACFYPYYSATNIDRLVSSGYNNSLPKEDLS 390
            .:|.:...|.:      .||...|:.:|     |..|               ||          |
Mouse   352 VLDRIAWILCLGEQPMAHRPPATFQANK-----TDDC---------------SG----------S 386

  Fly   391 QSITANGPFGGSCQVHGPVPPLTHCSSDEIAAVPDMDIGPLGMKSPILNNPAFSHSKCLPKIHKS 455
            ..:.|.|               .|||  .:....|::..|.|..||            ||...::
Mouse   387 DLLPAMG---------------NHCS--HVGGPQDLEKTPRGRGSP------------LPPPREA 422

  Fly   456 CFCVR-----------FIAEHTKMQEDSTKVKEDWKYVAMVLDRLFLWIFTLAVV 499
            ...||           |:.:..:|:|    |..||..|..|||||...|:.|||:
Mouse   423 SLAVRGLLQELSSIRHFLEKRDEMRE----VARDWLRVGYVLDRLLFRIYLLAVL 473

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha4	NP_001097669.2	LIC	1..505	CDD:455710	142/510 (28%)
		LGIC_ECD_nAChR_proto_alpha-like	11..229	CDD:349832	70/220 (32%)
		Cys-loop	136..150	CDD:349832	7/13 (54%)
Htr3a	NP_038589.2	LIC	5..483	CDD:273305	142/510 (28%)
		HA-stretch, determines single-channel conductance in 5-HT3 receptors. /evidence=ECO:0000250|UniProtKB:P46098	425..461		10/39 (26%)