Protein Alignment nAChRalpha4 and Chrne

DIOPT Version :9

• Sequence 1: NP_001097669.2
• Gene: nAChRalpha4 / 40521
• FlyBaseID: FBgn0266347
• Length: 539
• Species: Drosophila melanogaster
• Sequence 2: XP_006532069.1
• Gene: Chrne / 11448
• MGIID: 87894
• Length: 494
• Species: Mus musculus
• Alignment Length: 516
• Identity: 165/516 - (31%)
• Similarity: 262/516 - (50%)
• Gaps: 52/516 - (10%)

  Fly     4 GAVAGNPDAKRLYDDLLSNYNKLVRPVVNTTDVLKVCIKLKLSQLIDVNLKNQIMTTNLWVEQSW 68
            |...|..:...||..|..||:...|||....|.:.:.:|:.|:.||.:|.|.:.:||::|:...|
Mouse    16 GRSQGKNEELSLYHHLFDNYDPECRPVRRPEDTVTITLKVTLTNLISLNEKEETLTTSVWIGIDW 80

  Fly    69 YDYKLRWEPKEYGGVHMLHVPSDHIWRPDIVLYNNADGNFEVTLATKATIYSEGLVEWKPPAIYK 133
            :||:|.:...::.||.:|.|||:|:|.|:|||.||.||.|.|...:...:|..|.|.|.|||||:
Mouse    81 HDYRLNYSKDDFAGVGILRVPSEHVWLPEIVLENNIDGQFGVAYDSNVLVYEGGYVSWLPPAIYR 145

  Fly   134 SSCEIDVEYFPFDEQTCVLKFGSWTYDGFKVDLRHMDEQQGSNVVAVGVDLSEFYMSVEWDILEV 198
            |:|.::|.|||||.|.|.|.|.|.||:..:|:.....:..|:.:..:.:|.:.|..:.||.|...
Mouse   146 STCAVEVTYFPFDWQNCSLIFRSQTYNAEEVEFIFAVDDDGNTINKIDIDTAAFTENGEWAIDYC 210

  Fly   199 PA-VRNEKFYTCCDEPYLDITFNITMRRKTLFYTVNIIIPCMGISFLTVLTFYLPSDS-GEKVTL 261
            |. :|..:..:.......|:.:.:.:|||.|||.:|||:||:.||.|.:|.::||:.: |:|.|:
Mouse   211 PGMIRRYEGGSTEGPGETDVIYTLIIRRKPLFYVINIIVPCVLISGLVLLAYFLPAQAGGQKCTV 275

  Fly   262 SISILISLHVFFLLVVEIIPPTSLVVPLLGKYLIFAMILVSISICVTVVVLNVHFRSPQTHKMAP 326
            ||::|::..||..|:.:.||.|||.|||||:||||.|::.::.:...|:||||..|:|.||..:|
Mouse   276 SINVLLAQTVFLFLIAQKIPETSLSVPLLGRYLIFVMVVATLIVMNCVIVLNVSLRTPTTHATSP 340

  Fly   327 WVKRVFIDFLPAFLFIKRPTYNFETSKLLLKDTHACFYPYYSATNIDRLVSSGYNNSLPKEDLSQ 391
            .::::.::.||..|....|..:..|:.           |...|:::..|:.:  ...:.|:..|:
Mouse   341 RLRQILLELLPRLLGSSPPPEDPRTAS-----------PARRASSVGILLRA--EELILKKPRSE 392

  Fly   392 SITANGPFGGSCQVHGPVPPLTHCSSDEIAAVPDMDIGPLGMKSPILNNPAFSHSKCLPKIHKSC 456
            .:     |.|....||.......|.:...||                           |:|....
Mouse   393 LV-----FEGQRHRHGTWTAAALCQNLGAAA---------------------------PEIRCCV 425

  Fly   457 FCVRFIAEHTKMQEDSTKVKEDWKYVAMVLDRLFLWIFTLAVVVGTAGIIL-----QAPTL 512
            ..|.|:||.|:.||.:.:...||..:...||.:..|...:...||:..|.|     |.|.|
Mouse   426 DAVNFVAESTRDQEATGEELSDWVRMGKALDNVCFWAALVLFSVGSTLIFLGGYFNQVPDL 486

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha4	NP_001097669.2	LIC	1..505	CDD:273305	160/502 (32%)
		Neur_chan_LBD	12..227	CDD:280998	76/215 (35%)
		Neur_chan_memb	234..505	CDD:280999	77/271 (28%)
Chrne	XP_006532069.1	LGIC_ECD_nAChR_E	49..239	CDD:349831	66/189 (35%)
		Neur_chan_memb	247..474	CDD:367256	77/271 (28%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG3645
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	2	1.810