Protein Alignment nAChRalpha4 and Chrnb1

DIOPT Version :10

• Sequence 1: NP_001097669.2
• Gene: nAChRalpha4 / 40521
• FlyBaseID: FBgn0266347
• Length: 539
• Species: Drosophila melanogaster
• Sequence 2: NP_033731.3
• Gene: Chrnb1 / 11443
• MGIID: 87890
• Length: 501
• Species: Mus musculus
• Alignment Length: 518
• Identity: 182/518 - (35%)
• Similarity: 278/518 - (53%)
• Gaps: 66/518 - (12%)

  Fly     8 GNPDAKRLYDDLLSNYNKLVRPVVNTTDVLKVCIKLKLSQLIDVNLKNQIMTTNLWVEQSWYDYK 72
            |:....:|...|.|||:..|||.....|.:.|.|.|.|:|||.:|.|::.|:|.::::..|.||:
Mouse    23 GSEAEGQLIKKLFSNYDSSVRPAREVGDRVGVSIGLTLAQLISLNEKDEEMSTKVYLDLEWTDYR 87

  Fly    73 LRWEPKEYGGVHMLHVPSDHIWRPDIVLYNNADGNFEVTLATKATIYSEGLVEWKPPAIYKSSCE 137
            |.|:|.|:.|:..|.:.::.:|.||:||.||.||||:|.|.....:..||.|.|:||.:|:|||.
Mouse    88 LSWDPAEHDGIDSLRITAESVWLPDVVLLNNNDGNFDVALDINVVVSFEGSVRWQPPGLYRSSCS 152

  Fly   138 IDVEYFPFDEQTCVLKFGSWTYDGFKVDLRHMDEQQGSNVVAVGVDLSEFYMSVEWDILEVPA-- 200
            |.|.|||||.|.|.:.|.|::||..:|.|:...:.:|.....|.:....|..:.:|:|:..|:
Mouse   153 IQVTYFPFDWQNCTMVFSSYSYDSSEVSLKTGLDPEGEERQEVYIHEGTFIENGQWEIIHKPSRL 217

  Fly   201 ------VRNEKFYTCCDEPYLDITFNITMRRKTLFYTVNIIIPCMGISFLTVLTFYLPSDSGEKV 259
                  .|..|     :..:.::.|.:.:|||.|||.||:|.||:.|:.|.:..||||.|:|||:
Mouse   218 IQLPGDQRGGK-----EGHHEEVIFYLIIRRKPLFYLVNVIAPCILITLLAIFVFYLPPDAGEKM 277

  Fly   260 TLSISILISLHVFFLLVVEIIPPTSLVVPLLGKYLIFAMILVSISICVTVVVLNVHFRSPQTHKM 324
            .|||..|::|.||.||:.:.:|.|||.||::.|||:|.|:||:.|:.::|||||:|.|||.||:|
Mouse   278 GLSIFALLTLTVFLLLLADKVPETSLAVPIIIKYLMFTMVLVTFSVILSVVVLNLHHRSPHTHQM 342

  Fly   325 APWVKRVFIDFLPAFLFIKRPTYNFETSKLLLKDTHACFYPYYSATNIDRLVSSGYNNSL----- 384
            ..||:::||..||.:|.:|||    :..:..|.:.|....|           .||:....
Mouse   343 PFWVRQIFIHKLPPYLGLKRP----KPERDQLPEPHHSLSP-----------RSGWGRGTDEYFI 392

  Fly   385 --PKEDLSQSITANGPFGGSCQVHGPVPPLTHCSSDEIAAVPDMDIGPLGMKSPILNNPAFSHSK 447
              |..|..                  .|.|.....:  ::.||:        ...::.|  :.:.
Mouse   393 RKPPSDFL------------------FPKLNRFQPE--SSAPDL--------RRFIDGP--TRAV 427

  Fly   448 CLP-KIHKSCFCVRFIAEHTKMQEDSTKVKEDWKYVAMVLDRLFLWIFTLAVVVGTAGIILQA 509
            .|| ::.:....:.::|...:.|||...:||||::||||:||||||.|.:...|||..|.|.|
Mouse   428 GLPQELREVISSISYMARQLQEQEDHDALKEDWQFVAMVVDRLFLWTFIVFTSVGTLVIFLDA 490

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha4	NP_001097669.2	LIC	1..505	CDD:455710	179/512 (35%)
		LGIC_ECD_nAChR_proto_alpha-like	11..229	CDD:349832	79/225 (35%)
		Cys-loop	136..150	CDD:349832	9/13 (69%)
Chrnb1	NP_033731.3	LIC	24..488	CDD:455710	179/513 (35%)
		LGIC_ECD_nAChR_B1	27..245	CDD:349825	78/222 (35%)
		Cys-loop	151..165	CDD:349825	9/13 (69%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	362..382		5/34 (15%)