Protein Alignment nAChRalpha4 and Chrna7

DIOPT Version :10

• Sequence 1: NP_001097669.2
• Gene: nAChRalpha4 / 40521
• FlyBaseID: FBgn0266347
• Length: 539
• Species: Drosophila melanogaster
• Sequence 2: NP_031416.3
• Gene: Chrna7 / 11441
• MGIID: 99779
• Length: 502
• Species: Mus musculus
• Alignment Length: 545
• Identity: 208/545 - (38%)
• Similarity: 302/545 - (55%)
• Gaps: 75/545 - (13%)

  Fly     1 MVHGAVAGNPDAKRLYDDLLSNYNKLVRPVVNTTDVLKVCIKLKLSQLIDVNLKNQIMTTNLWVE 65
            ::|.::.|. ..:|||.:|:.|||.|.|||.|.:..|.|...|.|.|::||:.|||::|||:|::
Mouse    16 LLHVSLQGE-FQRRLYKELVKNYNPLERPVANDSQPLTVYFSLSLLQIMDVDEKNQVLTTNIWLQ 79

  Fly    66 QSWYDYKLRWEPKEYGGVHMLHVPSDHIWRPDIVLYNNADGNFEVTLATKATIYSEGLVEWKPPA 130
            .||.|:.|:|...||.||..:..|...||:|||:|||:||..|:.|..|...:.:.|..::.||.
Mouse    80 MSWTDHYLQWNMSEYPGVKNVRFPDGQIWKPDILLYNSADERFDATFHTNVLVNASGHCQYLPPG 144

  Fly   131 IYKSSCEIDVEYFPFDEQTCVLKFGSWTYDGFKVDLRHMDEQQGSNVVAVGVDLSEFYMSVEWDI 195
            |:||||.|||.:||||.|.|.||||||:|.|:.:||: |.|          .|:|.:..:.|||:
Mouse   145 IFKSSCYIDVRWFPFDVQQCKLKFGSWSYGGWSLDLQ-MQE----------ADISSYIPNGEWDL 198

  Fly   196 LEVPAVRNEKFYTCCDEPYLDITFNITMRRKTLFYTVNIIIPCMGISFLTVLTFYLPSDSGEKVT 260
            :.:|..||||||.||.|||.|:|:.:||||:||:|.:|::|||:.||.|.:|.|.||:|||||::
Mouse   199 MGIPGKRNEKFYECCKEPYPDVTYTVTMRRRTLYYGLNLLIPCVLISALALLVFLLPADSGEKIS 263

  Fly   261 LSISILISLHVFFLLVVEIIPPTSLVVPLLGKYLIFAMILVSISICVTVVVLNVHFRSPQTHKMA 325
            |.|::|:||.||.|||.||:|.||..|||:.:|....||:|.:|:.|||:||..|...|...||.
Mouse   264 LGITVLLSLTVFMLLVAEIMPATSDSVPLIAQYFASTMIIVGLSVVVTVIVLRYHHHDPDGGKMP 328

  Fly   326 PWVKRVFIDFLPAFLFIKRPTYNFETSKLLLKDTHACFYPYYSATNIDRLVSSGYNNSLPKEDLS 390
            .|.:.:.:::...||.:|||..:        |...||.:             .....||...:||
Mouse   329 KWTRIILLNWCAWFLRMKRPGED--------KVRPACQH-------------KPRRCSLASVELS 372

  Fly   391 QSI---TANGP--FGGSCQVHGPVPPLTHCSSDEIAAVPDMDI--GPLGMKSPILNNPAFSHSKC 448
            ...   |:||.  :.|...:.|     .||     |..||..:  |.|.. ||       :|.:.
Mouse   373 AGAGPPTSNGNLLYIGFRGLEG-----MHC-----APTPDSGVVCGRLAC-SP-------THDEH 419

  Fly   449 L----------PKIHKSCFCVRFIAEHTKMQEDSTKVKEDWKYVAMVLDRLFLWIFTLAVVVGTA 503
            |          |.:.|....||:||...:.|::|..:..:||:.|.|:|||.|..|::..::.|.
Mouse   420 LMHGTHPSDGDPDLAKILEEVRYIANRFRCQDESEVICSEWKFAACVVDRLCLMAFSVFTIICTI 484

  Fly   504 GIILQAPTLYDDRIPIIEQKDLDFS 528
            ||::.||.       .:|....||:
Mouse   485 GILMSAPN-------FVEAVSKDFA 502

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha4	NP_001097669.2	LIC	1..505	CDD:455710	201/520 (39%)
		LGIC_ECD_nAChR_proto_alpha-like	11..229	CDD:349832	102/217 (47%)
		Cys-loop	136..150	CDD:349832	9/13 (69%)
Chrna7	NP_031416.3	LIC	4..490	CDD:273305	203/524 (39%)
		Essential for TMEM35A/NACHO-mediated proper subunit assembly and trafficking to cell membrane. /evidence=ECO:0000269|PubMed:32783947	260..267		3/6 (50%)