Protein Alignment nAChRalpha4 and Chrna4

DIOPT Version :9

• Sequence 1: NP_001097669.2
• Gene: nAChRalpha4 / 40521
• FlyBaseID: FBgn0266347
• Length: 539
• Species: Drosophila melanogaster
• Sequence 2: NP_056545.3
• Gene: Chrna4 / 11438
• MGIID: 87888
• Length: 629
• Species: Mus musculus
• Alignment Length: 599
• Identity: 249/599 - (41%)
• Similarity: 331/599 - (55%)
• Gaps: 121/599 - (20%)

  Fly    13 KRLYDDLLSNYNKLVRPVVNTTDVLKVCIKLKLSQLIDVNLKNQIMTTNLWVEQSWYDYKLRWEP 77
            :||...|.|.|||..|||.|.:||:.|...|.::|||||:.|||:||||:||:|.|:||||||:|
Mouse    40 ERLLKRLFSGYNKWSRPVANISDVVLVRFGLSIAQLIDVDEKNQMMTTNVWVKQEWHDYKLRWDP 104

  Fly    78 KEYGGVHMLHVPSDHIWRPDIVLYNNADGNFEVTLATKATIYSEGLVEWKPPAIYKSSCEIDVEY 142
            .:|..|..:.:||:.||||||||||||||:|.||..|||.::.:|.|:|.|||||||||.|||.:
Mouse   105 GDYENVTSIRIPSELIWRPDIVLYNNADGDFAVTHLTKAHLFYDGRVQWTPPAIYKSSCSIDVTF 169

  Fly   143 FPFDEQTCVLKFGSWTYDGFKVDLRHMDEQQGSNVVAVGVDLSEFYMSVEWDILEVPAVRNEKFY 207
            ||||:|.|.:||||||||..|:||..|..:         ||..:|:.|.||.|::.....|.:.|
Mouse   170 FPFDQQNCTMKFGSWTYDKAKIDLVSMHSR---------VDQLDFWESGEWVIVDAVGTYNTRKY 225

  Fly   208 TCCDEPYLDITFNITMRRKTLFYTVNIIIPCMGISFLTVLTFYLPSDSGEKVTLSISILISLHVF 272
            .||.|.|.|||:...:||..||||:|:||||:.||.||||.|||||:.||||||.||:|:||.||
Mouse   226 ECCAEIYPDITYAFIIRRLPLFYTINLIIPCLLISCLTVLVFYLPSECGEKVTLCISVLLSLTVF 290

  Fly   273 FLLVVEIIPPTSLVVPLLGKYLIFAMILVSISICVTVVVLNVHFRSPQTHKMAPWVKRVFIDFLP 337
            .||:.||||.||||:||:|:||:|.||.|::||.:||.|||||.|||:||.|..||:|||:|.:|
Mouse   291 LLLITEIIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHHRSPRTHTMPAWVRRVFLDIVP 355

  Fly   338 AFLFIKRPTYNFETSKLLLKDTHAC-----FYPYYSA--------------------TNIDRLVS 377
            ..||:|||:...:..:.|::..|..     |:|...:                    ..:|..|.
Mouse   356 RLLFMKRPSVVKDNCRRLIESMHKMANAPRFWPEPESEPGILGDICNQGLSPAPTFCNRMDTAVE 420

  Fly   378 SGYNNSLPKE---DLSQSITANG---PFGGSCQVHGP------------------VP-------- 410
            :......|..   ||..|.....   |..|||  |.|                  ||
Mouse   421 TQPTCRSPSHKVPDLKTSEVEKASPCPSPGSC--HPPNSSGAPVLIKARSLSVQHVPSSQEAAEG 483

  Fly   411 -------PLTHCSSDEIAA--------------------VPDMD--------------IGPL--- 431
                   .:.:|.|.:.||                    :|..|              :.|:
Mouse   484 SIRCRSRSIQYCVSQDGAASLTESKPTGSPASLKTRPSQLPVSDQTSPCKCTCKEPSPVSPITVL 548

  Fly   432 ---GMKSPILNNPAFSHSKCLPKIHKSCFCVRFIAEHTKMQEDSTKVKEDWKYVAMVLDRLFLWI 493
               |.|:|    |  .|....|.:.::...|::||:|.|.::....|||||||||||:||:|||:
Mouse   549 KAGGTKAP----P--QHLPLSPALTRAVEGVQYIADHLKAEDTDFSVKEDWKYVAMVIDRIFLWM 607

  Fly   494 FTLAVVVGTAGIIL 507
            |.:..::||.|:.|
Mouse   608 FIIVCLLGTVGLFL 621

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha4	NP_001097669.2	LIC	1..505	CDD:273305	247/595 (42%)
		Neur_chan_LBD	12..227	CDD:280998	113/213 (53%)
		Neur_chan_memb	234..505	CDD:280999	129/374 (34%)
Chrna4	NP_056545.3	Neur_chan_LBD	39..245	CDD:280998	113/213 (53%)
		Neur_chan_memb	252..620	CDD:280999	130/375 (35%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	420..459		10/40 (25%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	503..529		2/25 (8%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	253	1.000	Domainoid score	I2060
eggNOG	1	0.900	-	-		E1_KOG3645
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	476	1.000	Inparanoid score	I1470
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	1	1.000	-	-		mtm8871
orthoMCL	1	0.900	-	-		OOG6_101158
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X294
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	9	8.760