Protein Alignment nAChRalpha4 and CHRNB3

DIOPT Version :9

• Sequence 1: NP_001097669.2
• Gene: nAChRalpha4 / 40521
• FlyBaseID: FBgn0266347
• Length: 539
• Species: Drosophila melanogaster
• Sequence 2: NP_000740.1
• Gene: CHRNB3 / 1142
• HGNCID: 1963
• Length: 458
• Species: Homo sapiens
• Alignment Length: 512
• Identity: 217/512 - (42%)
• Similarity: 297/512 - (58%)
• Gaps: 89/512 - (17%)

  Fly     5 AVAGNPDAKRLYDDLLSNYNKLVRPVVNTTDVLKVCIKLKLSQLIDVNLKNQIMTTNLWVEQSWY 69
            ::|.|.||  |...|...|.|.||||:::.|.:||...||:|||:||:.|||:||||:|::|.|.
Human    24 SIAENEDA--LLRHLFQGYQKWVRPVLHSNDTIKVYFGLKISQLVDVDEKNQLMTTNVWLKQEWT 86

  Fly    70 DYKLRWEPKEYGGVHMLHVPSDHIWRPDIVLYNNADGNFEVTLATKATIYSEGLVEWKPPAIYKS 134
            |:||||.|.:|||:|.:.|||:.:|.|||||:.||||.||.:|.||..:.|.|.|.|.|||.|||
Human    87 DHKLRWNPDDYGGIHSIKVPSESLWLPDIVLFENADGRFEGSLMTKVIVKSNGTVVWTPPASYKS 151

  Fly   135 SCEIDVEYFPFDEQTCVLKFGSWTYDGFKVDLRHMDEQQGSNVVAVGVDLSEFYMSVEWDILEVP 199
            ||.:||.:||||.|.|.:|||||||||..|||..::|         .||..:|:.:.||:||...
Human   152 SCTMDVTFFPFDRQNCSMKFGSWTYDGTMVDLILINE---------NVDRKDFFDNGEWEILNAK 207

  Fly   200 AVRNEKFYTCCDEPYLDITFNITMRRKTLFYTVNIIIPCMGISFLTVLTFYLPSDSGEKVTLSIS 264
            .::..:.......|:  ||::..:||..||||:.:||||:|:||||||.||||||.|||::||.|
Human   208 GMKGNRRDGVYSYPF--ITYSFVLRRLPLFYTLFLIIPCLGLSFLTVLVFYLPSDEGEKLSLSTS 270

  Fly   265 ILISLHVFFLLVVEIIPPTSLVVPLLGKYLIFAMILVSISICVTVVVLNVHFRSPQT-HKMAPWV 328
            :|:||.||.|::.||||.:|.|:||:|:||:|.||.|::||.|||.|:|||.||..| |.|||||
Human   271 VLVSLTVFLLVIEEIIPSSSKVIPLIGEYLLFIMIFVTLSIIVTVFVINVHHRSSSTYHPMAPWV 335

  Fly   329 KRVFIDFLPAFLFIKRPTYNFETSKLLLKDTHACFYPYYSATNIDRLVSSGYNNSLPKEDLSQSI 393
            ||:|:..||               |||....|           :||.       |.|:::.||.:
Human   336 KRLFLQKLP---------------KLLCMKDH-----------VDRY-------SSPEKEESQPV 367

  Fly   394 TANGPFGGSCQVHGPV---PPLTHCSSDEIAAVPDMDIGPLGMKSPILNNPAFSHSKCLPKIHKS 455
                       |.|.|   ......|..|...|                  ||        :.|:
Human   368 -----------VKGKVLEKKKQKQLSDGEKVLV------------------AF--------LEKA 395

  Fly   456 CFCVRFIAEHTKMQEDSTKVKEDWKYVAMVLDRLFLWIFTLAVVVGTAGIILQAPTL 512
            ...:|:|:.|.|.:...::|.:|||:||.||||:|||:|.:..|.|:  :::..|.|
Human   396 ADSIRYISRHVKKEHFISQVVQDWKFVAQVLDRIFLWLFLIVSVTGS--VLIFTPAL 450

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha4	NP_001097669.2	LIC	1..505	CDD:273305	215/503 (43%)
		Neur_chan_LBD	12..227	CDD:280998	102/214 (48%)
		Neur_chan_memb	234..505	CDD:280999	106/274 (39%)
CHRNB3	NP_000740.1	Neur_chan_LBD	23..449	CDD:332142	215/509 (42%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	251	1.000	Domainoid score	I2107
eggNOG	1	0.900	-	-		E1_KOG3645
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG45250
OrthoDB	1	1.010	-	-		D188416at33208
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	5	4.830