Protein Alignment nAChRalpha4 and CHRNB2

DIOPT Version :9

• Sequence 1: NP_001097669.2
• Gene: nAChRalpha4 / 40521
• FlyBaseID: FBgn0266347
• Length: 539
• Species: Drosophila melanogaster
• Sequence 2: NP_000739.1
• Gene: CHRNB2 / 1141
• HGNCID: 1962
• Length: 502
• Species: Homo sapiens
• Alignment Length: 505
• Identity: 221/505 - (43%)
• Similarity: 305/505 - (60%)
• Gaps: 49/505 - (9%)

  Fly     6 VAGNPDAKRLYDDLL--SNYNKLVRPVVNTTDVLKVCIKLKLSQLIDVNLKNQIMTTNLWVEQSW 68
            |.|....:||.:.||  |.||||:||..|.::::.|.:.:.|:|||.|:.:.||||||:|:.|.|
Human    23 VWGTDTEERLVEHLLDPSRYNKLIRPATNGSELVTVQLMVSLAQLISVHEREQIMTTNVWLTQEW 87

  Fly    69 YDYKLRWEPKEYGGVHMLHVPSDHIWRPDIVLYNNADGNFEVTLATKATIYSEGLVEWKPPAIYK 133
            .||:|.|:|:|:..:..:.:||.|||.||:||||||||.:||:..:.|.:..:|.:.|.||||||
Human    88 EDYRLTWKPEEFDNMKKVRLPSKHIWLPDVVLYNNADGMYEVSFYSNAVVSYDGSIFWLPPAIYK 152

  Fly   134 SSCEIDVEYFPFDEQTCVLKFGSWTYDGFKVDLRHMDEQQGSNVVAVGVDLSEFYMSVEWDILEV 198
            |:|:|:|::||||:|.|.:||.|||||..::||....|      ||   .|.:|..|.||||:.:
Human   153 SACKIEVKHFPFDQQNCTMKFRSWTYDRTEIDLVLKSE------VA---SLDDFTPSGEWDIVAL 208

  Fly   199 PAVRNEKFYTCCDEPYLDITFNITMRRKTLFYTVNIIIPCMGISFLTVLTFYLPSDSGEKVTLSI 263
            |..|||   ...|..|:|||::..:|||.||||:|:||||:.|:.|.:|.||||||.|||:||.|
Human   209 PGRRNE---NPDDSTYVDITYDFIIRRKPLFYTINLIIPCVLITSLAILVFYLPSDCGEKMTLCI 270

  Fly   264 SILISLHVFFLLVVEIIPPTSLVVPLLGKYLIFAMILVSISICVTVVVLNVHFRSPQTHKMAPWV 328
            |:|::|.||.||:.:|:|||||.|||:||||:|.|:||:.||..:|.|||||.|||.||.|||||
Human   271 SVLLALTVFLLLISKIVPPTSLDVPLVGKYLMFTMVLVTFSIVTSVCVLNVHHRSPTTHTMAPWV 335

  Fly   329 KRVFIDFLPAFLFIKRPTYNFETSKLLLKDTHACFYPYYSATNIDRLVSSGYNNSLPKEDLSQSI 393
            |.||::.|||.||:::|.::....:|.|:                       .....:|......
Human   336 KVVFLEKLPALLFMQQPRHHCARQRLRLR-----------------------RRQREREGAGALF 377

  Fly   394 TANGPFGGSCQVHGPVPPLTHCSSDEIAAVPDMDIGPLGMKSPILNNPAFSHSKCLPKIHKSCFC 458
            ....|...||...     :...|...:|       |..|.:...:..|..|...|...:.::...
Human   378 FREAPGADSCTCF-----VNRASVQGLA-------GAFGAEPAPVAGPGRSGEPCGCGLREAVDG 430

  Fly   459 VRFIAEHTKMQEDSTKVKEDWKYVAMVLDRLFLWIFTLAVVVGTAGIILQ 508
            |||||:|.:.::|...|.|||||||||:||||||||....|.||.|:.||
Human   431 VRFIADHMRSEDDDQSVSEDWKYVAMVIDRLFLWIFVFVCVFGTIGMFLQ 480

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha4	NP_001097669.2	LIC	1..505	CDD:273305	218/500 (44%)
		Neur_chan_LBD	12..227	CDD:280998	99/216 (46%)
		Neur_chan_memb	234..505	CDD:280999	111/270 (41%)
CHRNB2	NP_000739.1	LIC	29..479	CDD:273305	217/496 (44%)
		LGIC_ECD_nAChR_B2	31..234	CDD:349826	99/214 (46%)
		Cys-loop	155..169	CDD:349826	8/13 (62%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG3645
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D188416at33208
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	1	1.000	-	-		mtm8639
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	4	3.820