Protein Alignment nAChRalpha4 and CHRNB1

DIOPT Version :10

• Sequence 1: NP_001097669.2
• Gene: nAChRalpha4 / 40521
• FlyBaseID: FBgn0266347
• Length: 539
• Species: Drosophila melanogaster
• Sequence 2: NP_000738.2
• Gene: CHRNB1 / 1140
• HGNCID: 1961
• Length: 501
• Species: Homo sapiens
• Alignment Length: 531
• Identity: 188/531 - (35%)
• Similarity: 276/531 - (51%)
• Gaps: 88/531 - (16%)

  Fly     6 VAGNPDAKRLYDDLLSNYNKLVRPVVNTTDVLKVCIKLKLSQLIDVNLKNQIMTTNLWVEQSWYD 70
            |.|:....||.:.|.|.|:..|||.....|.::|.:.|.|:|||.:|.|::.|:|.::::..|.|
Human    21 VRGSEAEGRLREKLFSGYDSSVRPAREVGDRVRVSVGLILAQLISLNEKDEEMSTKVYLDLEWTD 85

  Fly    71 YKLRWEPKEYGGVHMLHVPSDHIWRPDIVLYNNADGNFEVTLATKATIYSEGLVEWKPPAIYKSS 135
            |:|.|:|.|:.|:..|.:.::.:|.||:||.||.||||:|.|.....:.|:|.|.|:||.||:||
Human    86 YRLSWDPAEHDGIDSLRITAESVWLPDVVLLNNNDGNFDVALDISVVVSSDGSVRWQPPGIYRSS 150

  Fly   136 CEIDVEYFPFDEQTCVLKFGSWTYDGFKVDLRHMDEQQGSNVVAVGVDLSEFYMSVEWDILEVPA 200
            |.|.|.|||||.|.|.:.|.|::||..:|.|:......|.....:.:....|..:.:|:|:..|:
Human   151 CSIQVTYFPFDWQNCTMVFSSYSYDSSEVSLQTGLGPDGQGHQEIHIHEGTFIENGQWEIIHKPS 215

  Fly   201 VRNEKFYTCCDEP-------YLDITFNITMRRKTLFYTVNIIIPCMGISFLTVLTFYLPSDSGEK 258
                :......:|       ..::.|.:.:|||.|||.||:|.||:.|:.|.:..||||.|:|||
Human   216 ----RLIQPPGDPRGGREGQRQEVIFYLIIRRKPLFYLVNVIAPCILITLLAIFVFYLPPDAGEK 276

  Fly   259 VTLSISILISLHVFFLLVVEIIPPTSLVVPLLGKYLIFAMILVSISICVTVVVLNVHFRSPQTHK 323
            :.|||..|::|.||.||:.:.:|.|||.||::.|||:|.|:||:.|:.::|||||:|.|||.||:
Human   277 MGLSIFALLTLTVFLLLLADKVPETSLSVPIIIKYLMFTMVLVTFSVILSVVVLNLHHRSPHTHQ 341

  Fly   324 MAPWVKRVFIDFLPAFLFIKRPTYNFETSKLLLKDTHACFYPYYSATNIDRLVSSGYNNSLPKED 388
            |..||:::||..||.:|.:|||.                                      |:.|
Human   342 MPLWVRQIFIHKLPLYLRLKRPK--------------------------------------PERD 368

  Fly   389 LSQSITANGPFGGSCQVHGPVPPLTHCSS---------DE--IAAVPDMDIGPLGMK-SPILNNP 441
            |.                 |.||  ||||         ||  |...|...:.|...: .|.|:.|
Human   369 LM-----------------PEPP--HCSSPGSGWGRGTDEYFIRKPPSDFLFPKPNRFQPELSAP 414

  Fly   442 AFSH--------SKCLPKIHKSCFCVRFIAEHTKMQEDSTKVKEDWKYVAMVLDRLFLWIFTLAV 498
            ....        ...||::.:....:.:||...:.|||...:||||::||||:||||||.|.:..
Human   415 DLRRFIDGPNRAVALLPELREVVSSISYIARQLQEQEDHDALKEDWQFVAMVVDRLFLWTFIIFT 479

  Fly   499 VVGTAGIILQA 509
            .|||..|.|.|
Human   480 SVGTLVIFLDA 490

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha4	NP_001097669.2	LIC	1..505	CDD:455710	185/525 (35%)
		LGIC_ECD_nAChR_proto_alpha-like	11..229	CDD:349832	77/224 (34%)
		Cys-loop	136..150	CDD:349832	9/13 (69%)
CHRNB1	NP_000738.2	LGIC_ECD_nAChR_B1	27..245	CDD:349825	76/221 (34%)
		Cys-loop	151..165	CDD:349825	9/13 (69%)
		Neur_chan_memb	252..487	CDD:460753	101/291 (35%)