Protein Alignment nAChRalpha4 and CHRNA5

DIOPT Version :10

• Sequence 1: NP_001097669.2
• Gene: nAChRalpha4 / 40521
• FlyBaseID: FBgn0266347
• Length: 539
• Species: Drosophila melanogaster
• Sequence 2: NP_000736.2
• Gene: CHRNA5 / 1138
• HGNCID: 1959
• Length: 468
• Species: Homo sapiens
• Alignment Length: 510
• Identity: 201/510 - (39%)
• Similarity: 286/510 - (56%)
• Gaps: 100/510 - (19%)

  Fly     5 AVAGNPDAKRLYDDLLSNYNKLVRPVVNTTDVLKVCIKLKLSQLIDVNLKNQIMTTNLWVEQSWY 69
            ::|.:.|:  |..||..:|.:.||||.:..|.:|:...|.:|||:||:.|||:||||:|::|.|.
Human    42 SIAKHEDS--LLKDLFQDYERWVRPVEHLNDKIKIKFGLAISQLVDVDEKNQLMTTNVWLKQEWI 104

  Fly    70 DYKLRWEPKEYGGVHMLHVPSDHIWRPDIVLYNNADGNFEVTLATKATIYSEGLVEWKPPAIYKS 134
            |.||||.|.:|||:.::.||||.:|.|||||::||||.||.| :||..|...|.|.|.|||.|||
Human   105 DVKLRWNPDDYGGIKVIRVPSDSVWTPDIVLFDNADGRFEGT-STKTVIRYNGTVTWTPPANYKS 168

  Fly   135 SCEIDVEYFPFDEQTCVLKFGSWTYDGFKVDLRHMDEQQGSNVVAVGVDLSEFYMSVEWDILEVP 199
            ||.|||.:||||.|.|.:|||||||||.:||:...|:.         ||..:|:.:.||:|:...
Human   169 SCTIDVTFFPFDLQNCSMKFGSWTYDGSQVDIILEDQD---------VDKRDFFDNGEWEIVSAT 224

  Fly   200 AVRNEKFYTCCDEPYLDITFNITMRRKTLFYTVNIIIPCMGISFLTVLTFYLPSDSGEKVTLSIS 264
            ..:..:..:||..||  :|::..::|..||||:.:||||:|:||||||.|||||:.|||:.|..|
Human   225 GSKGNRTDSCCWYPY--VTYSFVIKRLPLFYTLFLIIPCIGLSFLTVLVFYLPSNEGEKICLCTS 287

  Fly   265 ILISLHVFFLLVVEIIPPTSLVVPLLGKYLIFAMILVSISICVTVVVLNVHFRSPQTHK-MAPWV 328
            :|:||.||.|::.||||.:|.|:||:|:||:|.||.|::||.|||..:|:|.||..||. |||.|
Human   288 VLVSLTVFLLVIEEIIPSSSKVIPLIGEYLVFTMIFVTLSIMVTVFAINIHHRSSSTHNAMAPLV 352

  Fly   329 KRVFIDFLPAFLFIKRPTYNFETSKLLLKDTHACFYPYYSATNIDRLVSSGYNNSLPKEDLSQSI 393
            :::|:..||               |||...:|           :||..:.       ||:..
Human   353 RKIFLHTLP---------------KLLCMRSH-----------VDRYFTQ-------KEETE--- 381

  Fly   394 TANGPFGGSCQVHGPVPPLTHCSSDEIAAVPDMDIGPLGMKSPILNNPAFSHSKCLPKIHKSCFC 458
            :.:||               ..|.:.:.|..|                                .
Human   382 SGSGP---------------KSSRNTLEAALD--------------------------------S 399

  Fly   459 VRFIAEHTKMQEDSTKVKEDWKYVAMVLDRLFLWIFTLAVVVGTAGIILQAPTLY 513
            :|:|..|...:.|..:|.||||::|.||||:|||.|....:||:.|:.:  |.:|
Human   400 IRYITRHIMKENDVREVVEDWKFIAQVLDRMFLWTFLFVSIVGSLGLFV--PVIY 452

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha4	NP_001097669.2	LIC	1..505	CDD:455710	198/500 (40%)
		LGIC_ECD_nAChR_proto_alpha-like	11..229	CDD:349832	100/217 (46%)
		Cys-loop	136..150	CDD:349832	9/13 (69%)
CHRNA5	NP_000736.2	LGIC_ECD_nAChR_A5	45..251	CDD:349819	100/219 (46%)
		Cys-loop	170..184	CDD:349819	9/13 (69%)
		Neur_chan_memb	257..447	CDD:460753	94/272 (35%)