DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment nAChRalpha4 and CHRNA4

DIOPT Version :9

Sequence 1:	NP_001097669.2	Gene:	nAChRalpha4 / 40521	FlyBaseID:	FBgn0266347	Length:	539	Species:	Drosophila melanogaster
Sequence 2:	NP_000735.1	Gene:	CHRNA4 / 1137	HGNCID:	1958	Length:	627	Species:	Homo sapiens

Alignment Length:	591	Identity:	248/591 - (41%)
Similarity:	332/591 - (56%)	Gaps:	105/591 - (17%)

- Green bases have known domain annotations that are detailed below.

  Fly    13 KRLYDDLLSNYNKLVRPVVNTTDVLKVCIKLKLSQLIDVNLKNQIMTTNLWVEQSWYDYKLRWEP 77
            :||...|.|.|||..|||.|.:||:.|...|.::|||||:.|||:||||:||:|.|:||||||:|
Human    38 ERLLKKLFSGYNKWSRPVANISDVVLVRFGLSIAQLIDVDEKNQMMTTNVWVKQEWHDYKLRWDP 102

  Fly    78 KEYGGVHMLHVPSDHIWRPDIVLYNNADGNFEVTLATKATIYSEGLVEWKPPAIYKSSCEIDVEY 142
            .:|..|..:.:||:.||||||||||||||:|.||..|||.::.:|.|:|.|||||||||.|||.:
Human   103 ADYENVTSIRIPSELIWRPDIVLYNNADGDFAVTHLTKAHLFHDGRVQWTPPAIYKSSCSIDVTF 167

  Fly   143 FPFDEQTCVLKFGSWTYDGFKVDLRHMDEQQGSNVVAVGVDLSEFYMSVEWDILEVPAVRNEKFY 207
            ||||:|.|.:||||||||..|:||.:|..:         ||..:|:.|.||.|::.....|.:.|
Human   168 FPFDQQNCTMKFGSWTYDKAKIDLVNMHSR---------VDQLDFWESGEWVIVDAVGTYNTRKY 223

  Fly   208 TCCDEPYLDITFNITMRRKTLFYTVNIIIPCMGISFLTVLTFYLPSDSGEKVTLSISILISLHVF 272
            .||.|.|.|||:...:||..||||:|:||||:.||.||||.|||||:.|||:||.||:|:||.||
Human   224 ECCAEIYPDITYAFVIRRLPLFYTINLIIPCLLISCLTVLVFYLPSECGEKITLCISVLLSLTVF 288

  Fly   273 FLLVVEIIPPTSLVVPLLGKYLIFAMILVSISICVTVVVLNVHFRSPQTHKMAPWVKRVFIDFLP 337
            .||:.||||.||||:||:|:||:|.||.|::||.:||.|||||.|||:||.|..||:|||:|.:|
Human   289 LLLITEIIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHHRSPRTHTMPTWVRRVFLDIVP 353

  Fly   338 AFLFIKRPTYNFETSKLLLKDTHAC-----FY-------PYYSATN------------IDRLVSS 378
            ..|.:|||:...:..:.|::..|..     |:       |..|.|.            :|.....
Human   354 RLLLMKRPSVVKDNCRRLIESMHKMASAPRFWPEPEGEPPATSGTQSLHPPSPSFCVPLDVPAEP 418

  Fly   379 GYNNSLPKEDL--SQSITAN----GPFGGSCQ-VHGPVPP---------LTHCSSDEIA------ 421
            |.:...|.:.|  .|.:.|.    .|..|.|: .||...|         :.|.||...|
Human   419 GPSCKSPSDQLPPQQPLEAEKASPHPSPGPCRPPHGTQAPGLAKARSLSVQHMSSPGEAVEGGVR 483

  Fly   422 --------------AVPDMD---IGPLG----------------------MKSPILNNPAFS--- 444
                          |.|:.|   .|.|.                      .|.|...:|:.:
Human   484 CRSRSIQYCVPRDDAAPEADGQAAGALASRNTHSAELPPPDQPSPCKCTCKKEPSSVSPSATVKT 548

  Fly   445 --------HSKCLPKIHKSCFCVRFIAEHTKMQEDSTKVKEDWKYVAMVLDRLFLWIFTLAVVVG 501
                    |....|.:.::...|::||:|.|.::....|||||||||||:||:|||:|.:..::|
Human   549 RSTKAPPPHLPLSPALTRAVEGVQYIADHLKAEDTDFSVKEDWKYVAMVIDRIFLWMFIIVCLLG 613

  Fly   502 TAGIIL 507
            |.|:.|
Human   614 TVGLFL 619

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha4	NP_001097669.2	LIC	1..505	CDD:273305	246/587 (42%)
		Neur_chan_LBD	12..227	CDD:280998	113/213 (53%)
		Neur_chan_memb	234..505	CDD:280999	128/366 (35%)
CHRNA4	NP_000735.1	Neur_chan_LBD	37..243	CDD:280998	113/213 (53%)
		Neur_chan_memb	250..618	CDD:280999	129/367 (35%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	382..481		20/98 (20%)
		Ten_N	<423..>572	CDD:284015	24/148 (16%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	496..561		9/64 (14%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	251	1.000	Domainoid score	I2107
eggNOG	1	0.900	-	-		E1_KOG3645
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	485	1.000	Inparanoid score	I1448
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D188416at33208
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	1	1.000	-	-		mtm8639
orthoMCL	1	0.900	-	-		OOG6_101158
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X294
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	9	8.770