Protein Alignment nAChRalpha4 and CHRNA4

DIOPT Version :10

• Sequence 1: NP_001097669.2
• Gene: nAChRalpha4 / 40521
• FlyBaseID: FBgn0266347
• Length: 539
• Species: Drosophila melanogaster
• Sequence 2: NP_000735.1
• Gene: CHRNA4 / 1137
• HGNCID: 1958
• Length: 627
• Species: Homo sapiens
• Alignment Length: 591
• Identity: 248/591 - (41%)
• Similarity: 332/591 - (56%)
• Gaps: 105/591 - (17%)

  Fly    13 KRLYDDLLSNYNKLVRPVVNTTDVLKVCIKLKLSQLIDVNLKNQIMTTNLWVEQSWYDYKLRWEP 77
            :||...|.|.|||..|||.|.:||:.|...|.::|||||:.|||:||||:||:|.|:||||||:|
Human    38 ERLLKKLFSGYNKWSRPVANISDVVLVRFGLSIAQLIDVDEKNQMMTTNVWVKQEWHDYKLRWDP 102

  Fly    78 KEYGGVHMLHVPSDHIWRPDIVLYNNADGNFEVTLATKATIYSEGLVEWKPPAIYKSSCEIDVEY 142
            .:|..|..:.:||:.||||||||||||||:|.||..|||.::.:|.|:|.|||||||||.|||.:
Human   103 ADYENVTSIRIPSELIWRPDIVLYNNADGDFAVTHLTKAHLFHDGRVQWTPPAIYKSSCSIDVTF 167

  Fly   143 FPFDEQTCVLKFGSWTYDGFKVDLRHMDEQQGSNVVAVGVDLSEFYMSVEWDILEVPAVRNEKFY 207
            ||||:|.|.:||||||||..|:||.:|..:         ||..:|:.|.||.|::.....|.:.|
Human   168 FPFDQQNCTMKFGSWTYDKAKIDLVNMHSR---------VDQLDFWESGEWVIVDAVGTYNTRKY 223

  Fly   208 TCCDEPYLDITFNITMRRKTLFYTVNIIIPCMGISFLTVLTFYLPSDSGEKVTLSISILISLHVF 272
            .||.|.|.|||:...:||..||||:|:||||:.||.||||.|||||:.|||:||.||:|:||.||
Human   224 ECCAEIYPDITYAFVIRRLPLFYTINLIIPCLLISCLTVLVFYLPSECGEKITLCISVLLSLTVF 288

  Fly   273 FLLVVEIIPPTSLVVPLLGKYLIFAMILVSISICVTVVVLNVHFRSPQTHKMAPWVKRVFIDFLP 337
            .||:.||||.||||:||:|:||:|.||.|::||.:||.|||||.|||:||.|..||:|||:|.:|
Human   289 LLLITEIIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHHRSPRTHTMPTWVRRVFLDIVP 353

  Fly   338 AFLFIKRPTYNFETSKLLLKDTHAC-----FY-------PYYSATN------------IDRLVSS 378
            ..|.:|||:...:..:.|::..|..     |:       |..|.|.            :|.....
Human   354 RLLLMKRPSVVKDNCRRLIESMHKMASAPRFWPEPEGEPPATSGTQSLHPPSPSFCVPLDVPAEP 418

  Fly   379 GYNNSLPKEDL--SQSITAN----GPFGGSCQ-VHGPVPP---------LTHCSSDEIA------ 421
            |.:...|.:.|  .|.:.|.    .|..|.|: .||...|         :.|.||...|
Human   419 GPSCKSPSDQLPPQQPLEAEKASPHPSPGPCRPPHGTQAPGLAKARSLSVQHMSSPGEAVEGGVR 483

  Fly   422 --------------AVPDMD---IGPLG----------------------MKSPILNNPAFS--- 444
                          |.|:.|   .|.|.                      .|.|...:|:.:
Human   484 CRSRSIQYCVPRDDAAPEADGQAAGALASRNTHSAELPPPDQPSPCKCTCKKEPSSVSPSATVKT 548

  Fly   445 --------HSKCLPKIHKSCFCVRFIAEHTKMQEDSTKVKEDWKYVAMVLDRLFLWIFTLAVVVG 501
                    |....|.:.::...|::||:|.|.::....|||||||||||:||:|||:|.:..::|
Human   549 RSTKAPPPHLPLSPALTRAVEGVQYIADHLKAEDTDFSVKEDWKYVAMVIDRIFLWMFIIVCLLG 613

  Fly   502 TAGIIL 507
            |.|:.|
Human   614 TVGLFL 619

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha4	NP_001097669.2	LIC	1..505	CDD:455710	246/587 (42%)
		LGIC_ECD_nAChR_proto_alpha-like	11..229	CDD:349832	113/215 (53%)
		Cys-loop	136..150	CDD:349832	9/13 (69%)
CHRNA4	NP_000735.1	LGIC_ECD	37..243	CDD:475126	113/213 (53%)
		Neur_chan_memb	250..618	CDD:460753	129/367 (35%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	382..481		20/98 (20%)
		PRK14959	<392..496	CDD:184923	19/103 (18%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	496..561		9/64 (14%)