Protein Alignment nAChRalpha4 and CHRNA3

DIOPT Version :10

• Sequence 1: NP_001097669.2
• Gene: nAChRalpha4 / 40521
• FlyBaseID: FBgn0266347
• Length: 539
• Species: Drosophila melanogaster
• Sequence 2: NP_000734.2
• Gene: CHRNA3 / 1136
• HGNCID: 1957
• Length: 505
• Species: Homo sapiens
• Alignment Length: 534
• Identity: 232/534 - (43%)
• Similarity: 323/534 - (60%)
• Gaps: 89/534 - (16%)

  Fly    14 RLYDDLLSNYNKLVRPVVNTTDVLKVCIKLKLSQLIDVNLKNQIMTTNLWVEQSWYDYKLRWEPK 78
            ||::.|..:||:::|||.|.:|.:.:..::.:|||:.|:..||||.||||::|.|.||||:|.|.
Human    37 RLFERLFEDYNEIIRPVANVSDPVIIHFEVSMSQLVKVDEVNQIMETNLWLKQIWNDYKLKWNPS 101

  Fly    79 EYGGVHMLHVPSDHIWRPDIVLYNNADGNFEVTLATKATIYSEGLVEWKPPAIYKSSCEIDVEYF 143
            :|||...:.||:..||:|||||||||.|:|:|...|||.:...|.|.|.||||:||||:|||.||
Human   102 DYGGAEFMRVPAQKIWKPDIVLYNNAVGDFQVDDKTKALLKYTGEVTWIPPAIFKSSCKIDVTYF 166

  Fly   144 PFDEQTCVLKFGSWTYDGFKVDLRHMDEQQGSNVVAVG--VDLSEFYMSVEWDILEVPAVRNEKF 206
            |||.|.|.:|||||:||..|:||           |.:|  ::|.:::.|.||.|::.|..:::..
Human   167 PFDYQNCTMKFGSWSYDKAKIDL-----------VLIGSSMNLKDYWESGEWAIIKAPGYKHDIK 220

  Fly   207 YTCCDEPYLDITFNITMRRKTLFYTVNIIIPCMGISFLTVLTFYLPSDSGEKVTLSISILISLHV 271
            |.||:|.|.|||:::.:||..||||:|:||||:.|||||||.||||||.||||||.||:|:||.|
Human   221 YNCCEEIYPDITYSLYIRRLPLFYTINLIIPCLLISFLTVLVFYLPSDCGEKVTLCISVLLSLTV 285

  Fly   272 FFLLVVEIIPPTSLVVPLLGKYLIFAMILVSISICVTVVVLNVHFRSPQTHKMAPWVKRVFIDFL 336
            |.|::.|.||.||||:||:|:||:|.||.|::||.:||.|||||:|:|.||.|..|||.||::.|
Human   286 FLLVITETIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHYRTPTTHTMPSWVKTVFLNLL 350

  Fly   337 PAFLFIKRPT------------YNFETSKLLLKDTHACFYPYYSATNIDRLVSSGYNNSLPKEDL 389
            |..:|:.|||            |..|.|.|      .||         .|..|.|.....|.:|
Human   351 PRVMFMTRPTSNEGNAQKPRPLYGAELSNL------NCF---------SRAESKGCKEGYPCQD- 399

  Fly   390 SQSITANGPFGGSC--------QVHGPVPPLTHCSSDE-IAAVPDMDIGPLGMKSPILNNPAFSH 445
                       |.|        ::......||..||.| :.||                   .|.
Human   400 -----------GMCGYCHHRRIKISNFSANLTRSSSSESVDAV-------------------LSL 434

  Fly   446 SKCLPKIHKSCFCVRFIAEHTKMQEDSTKVKEDWKYVAMVLDRLFLWIFTLAVVVGTAGIILQAP 510
            |...|:|.::...|::|||:.|.|.::.::::||||||||:||:|||:|||..::||||:.||
Human   435 SALSPEIKEAIQSVKYIAENMKAQNEAKEIQDDWKYVAMVIDRIFLWVFTLVCILGTAGLFLQ-- 497

  Fly   511 TLYDDRIPIIEQKD 524
                   |::.::|
Human   498 -------PLMARED 504

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha4	NP_001097669.2	LIC	1..505	CDD:455710	227/513 (44%)
		LGIC_ECD_nAChR_proto_alpha-like	11..229	CDD:349832	101/216 (47%)
		Cys-loop	136..150	CDD:349832	10/13 (77%)
CHRNA3	NP_000734.2	LIC	30..496	CDD:455710	228/515 (44%)
		LGIC_ECD_nAChR_A3	35..241	CDD:349817	101/214 (47%)
		Cys-loop	159..173	CDD:349817	10/13 (77%)