Protein Alignment nAChRalpha4 and Chrna5

DIOPT Version :10

• Sequence 1: NP_001097669.2
• Gene: nAChRalpha4 / 40521
• FlyBaseID: FBgn0266347
• Length: 539
• Species: Drosophila melanogaster
• Sequence 2: NP_001360830.1
• Gene: Chrna5 / 110835
• MGIID: 87889
• Length: 467
• Species: Mus musculus
• Alignment Length: 518
• Identity: 204/518 - (39%)
• Similarity: 289/518 - (55%)
• Gaps: 106/518 - (20%)

  Fly     4 GAVAGNPD----AKR---LYDDLLSNYNKLVRPVVNTTDVLKVCIKLKLSQLIDVNLKNQIMTTN 61
            ||..|.|:    ||.   |:.||..:|.|.||||.:.:|.:|:...|.:|||:||:.|||:||||
Mouse    32 GARGGLPELSSAAKHEDSLFRDLFEDYEKWVRPVEHLSDKIKIKFGLAISQLVDVDEKNQLMTTN 96

  Fly    62 LWVEQSWYDYKLRWEPKEYGGVHMLHVPSDHIWRPDIVLYNNADGNFEVTLATKATIYSEGLVEW 126
            :|::|.|.|.||||.|.:|||:.::.||||.:|.|||||::||||.|| ..:||..:...|.|.|
Mouse    97 VWLKQEWIDVKLRWNPDDYGGIKIIRVPSDSLWIPDIVLFDNADGRFE-GASTKTVVRYNGTVTW 160

  Fly   127 KPPAIYKSSCEIDVEYFPFDEQTCVLKFGSWTYDGFKVDLRHMDEQQGSNVVAVGVDLSEFYMSV 191
            ..||.|||||.|||.:||||.|.|.:|||||||||.:||:...|:.         ||.::|:.:.
Mouse   161 TQPANYKSSCTIDVTFFPFDLQNCSMKFGSWTYDGSQVDIILEDQD---------VDRTDFFDNG 216

  Fly   192 EWDILEVPAVRNEKFYTCCDEPYLDITFNITMRRKTLFYTVNIIIPCMGISFLTVLTFYLPSDSG 256
            ||:|:.....:..:..:||..|.  ||::..::|..||||:.:||||:|:|||||:.|||||:.|
Mouse   217 EWEIMSAMGSKGNRTDSCCWYPC--ITYSFVIKRLPLFYTLFLIIPCIGLSFLTVVVFYLPSNEG 279

  Fly   257 EKVTLSISILISLHVFFLLVVEIIPPTSLVVPLLGKYLIFAMILVSISICVTVVVLNVHFRSPQT 321
            ||::|..|:|:||.||.|::.||||.:|.|:||:|:||:|.||.|::||.|||..:|:|.||..|
Mouse   280 EKISLCTSVLVSLTVFLLVIEEIIPSSSKVIPLIGEYLVFTMIFVTLSIMVTVFAINIHHRSSST 344

  Fly   322 HK-MAPWVKRVFIDFLPAFLFIKRPTYNFETSKLLLKDTHACFYPYYSATNIDRLVSSGYNNSLP 385
            |. |||||:::|:..||               |||...:||           ||..:.
Mouse   345 HNAMAPWVRKIFLHKLP---------------KLLCMRSHA-----------DRYFTQ------- 376

  Fly   386 KEDLSQSITANGPFGGSCQVHGPVPPLTHCSSDEIAAVPDMDIGPLGMKSPILNNPAFSHSKCLP 450
            :|:                                   .:.|.||                |...
Mouse   377 REE-----------------------------------AEKDGGP----------------KSRN 390

  Fly   451 KIHKSCFCVRFIAEHTKMQEDSTKVKEDWKYVAMVLDRLFLWIFTLAVVVGTAGIILQAPTLY 513
            .:..:..|:|:|..|...:.|..:|.||||::|.||||:|||.|.|..::||.|:.:  |.:|
Mouse   391 TLEAALDCIRYITRHVVKENDVREVVEDWKFIAQVLDRMFLWTFLLVSIIGTLGLFV--PVIY 451

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha4	NP_001097669.2	LIC	1..505	CDD:455710	201/508 (40%)
		LGIC_ECD_nAChR_proto_alpha-like	11..229	CDD:349832	99/224 (44%)
		Cys-loop	136..150	CDD:349832	9/13 (69%)
Chrna5	NP_001360830.1	LGIC_ECD	45..251	CDD:475126	98/217 (45%)
		Neur_chan_memb	257..446	CDD:460753	95/272 (35%)