Protein Alignment nAChRalpha4 and Chrna3

DIOPT Version :9

• Sequence 1: NP_001097669.2
• Gene: nAChRalpha4 / 40521
• FlyBaseID: FBgn0266347
• Length: 539
• Species: Drosophila melanogaster
• Sequence 2: NP_660111.3
• Gene: Chrna3 / 110834
• MGIID: 87887
• Length: 499
• Species: Mus musculus
• Alignment Length: 534
• Identity: 231/534 - (43%)
• Similarity: 327/534 - (61%)
• Gaps: 81/534 - (15%)

  Fly     6 VAGNPDAK-RLYDDLLSNYNKLVRPVVNTTDVLKVCIKLKLSQLIDVNLKNQIMTTNLWVEQSWY 69
            ||...:|: ||:..|..:||:::|||.|.:..:.:..::.:|||:.|:..||||.||||::|.|.
Mouse    22 VASASEAEHRLFQYLFEDYNEIIRPVANVSHPVIIQFEVSMSQLVKVDEVNQIMETNLWLKQIWN 86

  Fly    70 DYKLRWEPKEYGGVHMLHVPSDHIWRPDIVLYNNADGNFEVTLATKATIYSEGLVEWKPPAIYKS 134
            ||||:|:|.:|.||..:.||::.||:|||||||||||:|:|...|||.:...|.|.|.||||:||
Mouse    87 DYKLKWKPSDYQGVEFMRVPAEKIWKPDIVLYNNADGDFQVDDKTKALLKYTGEVTWIPPAIFKS 151

  Fly   135 SCEIDVEYFPFDEQTCVLKFGSWTYDGFKVDLRHMDEQQGSNVVAVG--VDLSEFYMSVEWDILE 197
            ||:|||.|||||.|.|.:|||||:||..|:||           |.:|  ::|.:::.|.||.|::
Mouse   152 SCKIDVTYFPFDYQNCTMKFGSWSYDKAKIDL-----------VLIGSSMNLKDYWESGEWAIIK 205

  Fly   198 VPAVRNEKFYTCCDEPYLDITFNITMRRKTLFYTVNIIIPCMGISFLTVLTFYLPSDSGEKVTLS 262
            .|..::|..|.||:|.|.|||:::.:||..||||:|:||||:.|||||||.||||||.||||||.
Mouse   206 APGYKHEIKYNCCEEIYQDITYSLYIRRLPLFYTINLIIPCLLISFLTVLVFYLPSDCGEKVTLC 270

  Fly   263 ISILISLHVFFLLVVEIIPPTSLVVPLLGKYLIFAMILVSISICVTVVVLNVHFRSPQTHKMAPW 327
            ||:|:||.||.|::.|.||.||||:||:|:||:|.||.|::||.:||.|||||:|:|.||.|..|
Mouse   271 ISVLLSLTVFLLVITETIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHYRTPTTHTMPTW 335

  Fly   328 VKRVFIDFLPAFLFIKRPT------------YNFETSKL---LLKDTHAC--FYPYYSAT----N 371
            ||.||::.||..:|:.|||            |..|.|.|   ...|:.:|  .||....|    :
Mouse   336 VKAVFLNLLPRVMFMTRPTSTEEDAPKTRNFYGAELSNLNCFSRADSKSCKEGYPCQDGTCGYCH 400

  Fly   372 IDRLVSSGYNNSLPKEDLSQSITANGPFGGSCQVHGPVPPLTHCSSDEIAAVPDMDIGPLGMKSP 436
            ..|:..|.::.:|.:...|:|:.|
Mouse   401 HRRVKISNFSANLTRSSSSESVDA----------------------------------------- 424

  Fly   437 ILNNPAFSHSKCLPKIHKSCFCVRFIAEHTKMQEDSTKVKEDWKYVAMVLDRLFLWIFTLAVVVG 501
            :|:..|.|     |:|.::...|::|||:.|.|..:.::::||||||||:||:|||:|.|..::|
Mouse   425 VLSLSALS-----PEIKEAIQSVKYIAENMKAQNVAKEIQDDWKYVAMVIDRIFLWVFILVCILG 484

  Fly   502 TAGIILQAPTLYDD 515
            |||:.||.....||
Mouse   485 TAGLFLQPLMARDD 498

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha4	NP_001097669.2	LIC	1..505	CDD:273305	226/522 (43%)
		Neur_chan_LBD	12..227	CDD:280998	103/217 (47%)
		Neur_chan_memb	234..505	CDD:280999	116/291 (40%)
Chrna3	NP_660111.3	LIC	24..490	CDD:273305	225/522 (43%)
		LGIC_ECD_nAChR_A3	29..235	CDD:349817	102/216 (47%)
		Cys-loop	153..167	CDD:349817	10/13 (77%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	253	1.000	Domainoid score	I2060
eggNOG	1	0.900	-	-		E1_KOG3645
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H591
Inparanoid	1	1.050	476	1.000	Inparanoid score	I1470
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG45250
OrthoDB	1	1.010	-	-		D188416at33208
OrthoFinder	1	1.000	-	-		FOG0000583
OrthoInspector	1	1.000	-	-		mtm8871
orthoMCL	1	0.900	-	-		OOG6_101158
Panther	1	1.100	-	-	O	PTHR18945
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R2381
SonicParanoid	1	1.000	-	-		X294
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	15	14.910