Protein Alignment nAChRalpha4 and Chrnb4

DIOPT Version :9

• Sequence 1: NP_001097669.2
• Gene: nAChRalpha4 / 40521
• FlyBaseID: FBgn0266347
• Length: 539
• Species: Drosophila melanogaster
• Sequence 2: NP_683746.1
• Gene: Chrnb4 / 108015
• MGIID: 87892
• Length: 495
• Species: Mus musculus
• Alignment Length: 529
• Identity: 215/529 - (40%)
• Similarity: 312/529 - (58%)
• Gaps: 87/529 - (16%)

  Fly    13 KRLYDDLL--SNYNKLVRPVVNTTDVLKVCIKLKLSQLIDVNLKNQIMTTNLWVEQSWYDYKLRW 75
            ::|.||||  :.||.|:||..:::.::.:.::|.|||||.||.:.|||||::|::|.|.||:|.|
Mouse    27 EKLMDDLLNKTRYNNLIRPATSSSQLISIRLELSLSQLISVNEREQIMTTSIWLKQEWTDYRLAW 91

  Fly    76 EPKEYGGVHMLHVPSDHIWRPDIVLYNNADGNFEVTLATKATIYSEGLVEWKPPAIYKSSCEIDV 140
            ....|.||::|.:|:..:|.|||||||||||.:||::.|...:.|.|.::|.|||||||:|:|:|
Mouse    92 NSSCYEGVNILRIPAKRVWLPDIVLYNNADGTYEVSVYTNVIVRSNGSIQWLPPAIYKSACKIEV 156

  Fly   141 EYFPFDEQTCVLKFGSWTYDGFKVDLRHMDEQQGSNVVAVGVDLSEFYMSVEWDILEVPAVR--N 203
            ::||||:|.|.|||.|||||..::|:      ...:..|:   :.:|..|.||||:.:|..|  |
Mouse   157 KHFPFDQQNCTLKFRSWTYDHTEIDM------VLKSPTAI---MDDFTPSGEWDIVALPGRRTVN 212

  Fly   204 EKFYTCCDEPYLDITFNITMRRKTLFYTVNIIIPCMGISFLTVLTFYLPSDSGEKVTLSISILIS 268
            .:     |..|:|:|::..::||.||||:|:||||:.|:.|.:|.||||||.|||:||.||:|::
Mouse   213 PQ-----DPSYVDVTYDFIIKRKPLFYTINLIIPCVLITSLAILVFYLPSDCGEKMTLCISVLLA 272

  Fly   269 LHVFFLLVVEIIPPTSLVVPLLGKYLIFAMILVSISICVTVVVLNVHFRSPQTHKMAPWVKRVFI 333
            |..|.||:.:|:|||||.:||:||||:|.|:||:.||..||.|||||.|||.||.||.|||..|:
Mouse   273 LTFFLLLISKIVPPTSLDIPLIGKYLLFTMVLVTFSIVTTVCVLNVHHRSPSTHTMASWVKECFL 337

  Fly   334 DFLPAFLFIKRPTYNFETSKLLLKDTHACFYPYYSATNIDRLVSSGYNNSLPKEDLSQSITANGP 398
            ..||.|||:|||......:::          |:.|..::....::             |.:|.||
Mouse   338 HKLPTFLFMKRPGLEVSPARV----------PHSSQLHLTTAEAT-------------STSALGP 379

  Fly   399 ------FGGSCQVHGPVPPLTHCSSDEIAAVPDMDIGPLGMKSPILNNPAFSHSKCLPK------ 451
                  :|.|.....|||           |.|         ||.:     .||:..||:
Mouse   380 SSPSNLYGNSMYFVNPVP-----------ATP---------KSAV-----SSHTAGLPRDARLRS 419

  Fly   452 -------IHKSCFCVRFIAEHTKMQEDSTKVKEDWKYVAMVLDRLFLWIFTLAVVVGTAGIILQA 509
                   :.::...|.|||:|.:..:....|.||||:||||:||||||:|.:..::||.|:.|
Mouse   420 SGRFRQDLQEALEGVSFIAQHLESDDRDQSVIEDWKFVAMVVDRLFLWVFVIVCILGTMGLFL-- 482

  Fly   510 PTLYDDRIP 518
            |.|:....|
Mouse   483 PPLFQIHAP 491

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha4	NP_001097669.2	LIC	1..505	CDD:273305	210/514 (41%)
		Neur_chan_LBD	12..227	CDD:280998	93/217 (43%)
		Neur_chan_memb	234..505	CDD:280999	111/289 (38%)
Chrnb4	NP_683746.1	LIC	21..483	CDD:273305	212/519 (41%)
		Neur_chan_LBD	26..231	CDD:280998	93/217 (43%)
		Neur_chan_memb	238..481	CDD:280999	112/290 (39%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG3645
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D188416at33208
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	1	1.000	-	-		mtm11113
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	5	4.820