Protein Alignment nAChRalpha4 and chrna8

DIOPT Version :10

• Sequence 1: NP_001097669.2
• Gene: nAChRalpha4 / 40521
• FlyBaseID: FBgn0266347
• Length: 539
• Species: Drosophila melanogaster
• Sequence 2: XP_021329572.1
• Gene: chrna8 / 100537531
• ZFINID: ZDB-GENE-090313-161
• Length: 516
• Species: Danio rerio
• Alignment Length: 554
• Identity: 204/554 - (36%)
• Similarity: 303/554 - (54%)
• Gaps: 87/554 - (15%)

  Fly     2 VHGAVAGNPDAKRLYDDLLSNYNKLVRPVVNTTDVLKVCIKLKLSQLIDVNLKNQIMTTNLWVEQ 66
            :.|::.|... ::||.:|:.|||:|.|||:|.:..:.|.:.|.|.|:|||:.|||::.||.|::.
Zfish    23 IKGSLQGEYQ-RKLYKELMENYNRLERPVLNDSAPIVVELGLTLLQIIDVDEKNQVLITNAWLQL 86

  Fly    67 SWYDYKLRWEPKEYGGVHMLHVPSDHIWRPDIVLYNNADGNFEVTLATKATIYSEGLVEWKPPAI 131
            .|.|..|.|.|..|.||..|..||:.||.|||:|||:||..|:.|..|...:.:.|..::.||.|
Zfish    87 GWTDVYLSWNPDNYPGVQTLRFPSNQIWTPDILLYNSADERFDATFHTNVLVNASGACQYIPPGI 151

  Fly   132 YKSSCEIDVEYFPFDEQTCVLKFGSWTYDGFKVDLRHMDEQQGSNVVAVGVDLSEFYMSVEWDIL 196
            .||:|.|||.:||||.|.|.|||||||::|:.:||:..           .||:|.:..:.|||::
Zfish   152 LKSTCYIDVRWFPFDIQKCDLKFGSWTHNGWLLDLQMQ-----------AVDISTYIPNGEWDLV 205

  Fly   197 EVPAVRNEKFYTCCDEPYLDITFNITMRRKTLFYTVNIIIPCMGISFLTVLTFYLPSDSGEKVTL 261
            .||..|||.:|.||.|||.|:||.:||||:||:|.:|::|||:.||.|.:|.|.||:|||||::|
Zfish   206 GVPGKRNELYYECCKEPYPDVTFTVTMRRRTLYYGLNLLIPCVLISGLALLVFLLPADSGEKISL 270

  Fly   262 SISILISLHVFFLLVVEIIPPTSLVVPLLGKYLIFAMILVSISICVTVVVLNVHFRSPQTHKMAP 326
            .|::|:||.||.|||.||:|.||..|||:.:|....|::|.:|:.|||:||..|...||..||..
Zfish   271 GITVLLSLTVFMLLVAEIMPATSDSVPLIAQYFASTMMIVGLSVVVTVLVLQFHHHDPQGGKMPR 335

  Fly   327 WVKRVFIDFLPAFLFIKRP------TYNFETSKLLLKDTHACFYPYYSATNIDRLVSSGYNNSLP 385
            ||:.:.:::...||.:|:|      .:.::.|..    .||      ||::|...|       :|
Zfish   336 WVRVILLNWCAWFLRMKKPGEERKANFKYDQSPA----HHA------SASSIQMGV-------MP 383

  Fly   386 KEDLSQSITANGP----FG-----GSCQVHGPVPPLT----HCSSDEIAAVPD--------MDIG 429
            .:..|.:.|.||.    ||     .:|     .||.:    .||...:...|:        |..|
Zfish   384 GQPSSTNSTTNGHMNLYFGYHSIDSAC-----CPPSSDSGVSCSGGRVGGSPNEEQREGVRMFAG 443

  Fly   430 PLGMKSPILNNPAFSHSKCLPKIHKSCFCVRFIAEHTKMQEDSTKVKEDWKYVAMVLDRLFLWIF 494
            .                   |:||:....|.:||:..:.|:::..:..:||:.|.|:|||.|..|
Zfish   444 E-------------------PEIHRILAEVMYIAQRFRDQDEAEAICSEWKFAAAVVDRLCLVAF 489

  Fly   495 TLAVVVGTAGIILQAPTLYDDRIPIIEQKDLDFS 528
            :|..::.|..|::.||.       .||....||:
Zfish   490 SLFSIICTFTILMSAPN-------FIEAVSKDFT 516

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha4	NP_001097669.2	LIC	1..505	CDD:455710	197/529 (37%)
		LGIC_ECD_nAChR_proto_alpha-like	11..229	CDD:349832	99/217 (46%)
		Cys-loop	136..150	CDD:349832	9/13 (69%)
chrna8	XP_021329572.1	LIC	12..504	CDD:455710	198/533 (37%)